Recombinant Human His6-UFM1 Activating Enzyme (UBA5), CF
Recombinant Human His6-UFM1 Activating Enzyme (UBA5), CF Summary
Product Specifications
Contains an N-terminal 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-319
| Formulation | 0.24 mg/ml (5 μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UFM1 Activating Enzyme/UBA5
Ubiquitin-fold Modifier 1 (UFM1) Activating Enzyme, also known as Ubiquitin-like Modifier Activating Enzyme 5 (UBA5) and Ubiquitin-activating Enzyme E1 Domain-containing Protein 1 (UBE1DC1), is a 404 amino acid (aa) member of the Ubiquitin-activating (E1) enzyme family with a predicted molecular weight of 45 kDa (1). The mouse and rat UFM1 Activating Enzyme/UBA5 orthologs share 87% and 88% aa sequence identity with the human protein, respectively. UFM1 Activating Enzyme/UBA5 is highly conserved in plants and metazoans but not in yeast (2). In humans, it is expressed both as the full length protein and as a truncated isoform that lacks aa 1-56. It is widely expressed in human cells where it is localized to the cytoplasm and nucleus (1,3). UFM1 Activating Enzyme/UBA5 has a conserved ATP-binding motif in close proximity to an active site cysteine residue, Cys250 in humans, and a metal binding motif, both of which are common to most E1 enzymes (2). It is the activating enzyme for both UFM1 and SUMO2 (3). UFM1 Activating Enzyme/UBA5 is required for erythroid differentiation in mice (4). This protein has an N-terminal His6 tag.
- Dou, T. et al. (2005) Mol. Biol. Rep. 32:265.
- Komatsu, M. et al. (2004) EMBO J. 23:1977.
- Zheng, M. et al. (2008) J. Cell. Biochem. 104:2324.
- Tatsumi, K. et al. (2011) Nat. Commun. 2:181.
Citation for Recombinant Human His6-UFM1 Activating Enzyme (UBA5), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination
Authors: J Liu, D Guan, M Dong, J Yang, H Wei, Q Liang, L Song, L Xu, J Bai, C Liu, J Mao, Q Zhang, J Zhou, X Wu, M Wang, YS Cong
Nat. Cell Biol., 2020-08-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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