Recombinant Human His6-USP28 Protein, CF

Catalog # Availability Size / Price Qty
E-570-100
Product Details
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Recombinant Human His6-USP28 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human USP28 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP28 concentration of 10-500 nM.  
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP28 protein
Contains a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
123 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-570

Formulation

X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP28

USP28 is a deubiquitinating enzyme of the C19 peptidase family that regulates MYC stability and DNA damage response checkpoints. USP28 regulates DNA repair by deubiquitinating and thereby stabilizing CLSPN - a protein that is ubiquitinated by the APC/C E3 Ubiquitin ligase in a cell-cycle dependent manner. USP28 interacts with isoform 1 of FBXW7 in the nucleoplasm, and regulates MYC degradation by counteracting its ubiquitination. Recently, a potential role in HIF-1 alpha -dependent processes such as angiogenesis and metastasis has been described for this deubiquitinase. USP28 has been demonstrated to cleave K6, K11, K48, and K63-linked poly-Ubiquitin chains. This recombinant protein contains a C-terminal 6-His tag.

 

 

References
  1. Basserman F., et al. (2008) Cell 134: 256-267.
  2. Flügel D., et al. (2012) Blood 119:1292-301.
  3. Popov N., et al. (2007) Cell Cycle 6: 2327-2331.
  4. Zhang D., et al. (2006) Cell 126: 529-542.
Long Name
Ubiquitin Specific Protease 28
Entrez Gene IDs
57646 (Human); 235323 (Mouse); 315639 (Rat)
Alternate Names
Deubiquitinating enzyme 28; EC 3.1.2.15; EC 3.4.19.12; KIAA1515ubiquitin carboxyl-terminal hydrolase 28; ubiquitin specific peptidase 28; ubiquitin specific protease 28; ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; USP28

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