Recombinant Human His6-USP28 Protein, CF

R&D Systems | Catalog # E-570

R&D Systems
Discontinued Product
E-570 has been discontinued. View all USP28 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q96RU2

Applications

Bioactivity
View all USP28 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP28 protein
Contains a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

123 kDa

Activity

Recombinant Human USP28 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP28 concentration of 10-500 nM.  

Formulation, Preparation, and Storage

E-570
Formulation

X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP28

USP28 is a deubiquitinating enzyme of the C19 peptidase family that regulates MYC stability and DNA damage response checkpoints. USP28 regulates DNA repair by deubiquitinating and thereby stabilizing CLSPN - a protein that is ubiquitinated by the APC/C E3 Ubiquitin ligase in a cell-cycle dependent manner. USP28 interacts with isoform 1 of FBXW7 in the nucleoplasm, and regulates MYC degradation by counteracting its ubiquitination. Recently, a potential role in HIF-1 alpha -dependent processes such as angiogenesis and metastasis has been described for this deubiquitinase. USP28 has been demonstrated to cleave K6, K11, K48, and K63-linked poly-Ubiquitin chains. This recombinant protein contains a C-terminal 6-His tag.

 

 

References

  1. Basserman F., et al. (2008) Cell 134: 256-267.
  2. Flügel D., et al. (2012) Blood 119:1292-301.
  3. Popov N., et al. (2007) Cell Cycle 6: 2327-2331.
  4. Zhang D., et al. (2006) Cell 126: 529-542.

Long Name

Ubiquitin Specific Protease 28

Alternate Names

Deubiquitinating enzyme 28, EC 3.1.2.15, EC 3.4.19.12, KIAA1515ubiquitin carboxyl-terminal hydrolase 28, ubiquitin specific peptidase 28, ubiquitin specific protease 28, ubiquitin thioesterase 28, Ubiquitin thiolesterase 28, Ubiquitin-specific-processing protease 28

Entrez Gene IDs

57646 (Human); 235323 (Mouse); 315639 (Rat)

Gene Symbol

USP28

UniProt

Q96RU2

Additional USP28 Products

Product Documents for Recombinant Human His6-USP28 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP28 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP28 Protein, CF

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