Recombinant Human HSP60 Protein, CF

Catalog # Availability Size / Price Qty
AP-140-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human HSP60 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

HSP60/HSPD1 is a molecular chaperone that assists in the folding of nascent polypeptides and the refolding of denatured proteins. Reaction conditions will need to be optimized for each specific application.  We recommend an initial HSP60/HSPD1 concentration of 2-3 μM for in vitro use. IMPORTANT: HSP10/HSPE1 (Catalog # AP-150) is required for HSP60/HSPD1 activity and should be used at a concentration that is at least equimolar to HSP60/HSPD1.

Source
E. coli-derived human HSP60 protein
Accession # P10809
Accession #
Predicted Molecular Mass

61 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-140

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP60

HSP60 (also known as Chaperonin 60) is the eukaryotic homologue of prokaryotic GroEL chaperone. This protein is found mainly in mitochondria, but can also be detected in cytosol and extracellular fluids including peripheral blood. HSP60 and HSP10 (also known as Chaperonin 10) form a complex that plays an essential role in the translocation and refolding of proteins from the cytosol into the mitochondrial matrix. Under physiological conditions, HSP60 creates two stacked heptameric rings that form a pair of central hydrophobic cavities. After an unfolded substrate protein enters one of the cavities it is capped by a heptameric HSP10 complex, thereby trapping the unfolded protein. Structural rearrangement of the substrate-containing cavity is effected via HSP60-mediated ATP hydrolysis; this changes the lining of the cavity from hydrophobic to hydrophilic and helps promote refolding of the substrate protein. Binding of ATP to HSP60 subunits on the distal ring of the complex then causes the dissociation of the HSP10 cap complex and concomitant release of the substrate protein from the proximal cavity. If the protein is not completely folded, it can be further processed by the HSP60/HSP10 complex, or can interact with other chaperoning systems.

References
  1. Cappello F, et al. (2008) Cancer Biol. Therapy 7: 801-809
  2. Hartl F.U. & Hayer-Hartl M. (2009) Nat. Struc. Mol. Biol. 16: 574-581
Long Name
Heat Shock Protein 60
Entrez Gene IDs
3329 (Human); 110907 (Mouse); 63868 (Rat)
Alternate Names
60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13

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Recombinant Human HSP60 Protein, CF
By Anonymous on 08/17/2018
Application: In vitro bioactivity in cell culture