Recombinant Human IL-17B (CHO) Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
8129-IL-025/CF
Recombinant Human IL-17B (CHO) Protein, CF Bioactivity
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Recombinant Human IL-17B (CHO) Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. Tang, Y. et al. (2011) Clin. Exp. Immunol. 166:281. The ED50 for this effect is 0.4-2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-17B protein
Gln21-Phe180
Accession #
N-terminal Sequence
Analysis
Gln21, Ser51 & Gly102
Gln21 predicted, sequencing might be blocked
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
18 kDa (monomer)
SDS-PAGE
20-25 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8129-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8129-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human IL-17B (CHO) Protein, CF Bioactivity View Larger

Recombinant Human IL-17B induces IL-8 secretion by HepG2 human hepatocellular carcinoma cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17B

Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17B shares 90% and 91% amino acid sequence identity with mouse and rat IL-17B, respectively (3, 4). IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine (3-5). In the skeletal system, IL-17B is expressed in bone collars surrounding primary ossification centers as well as in epiphyseal plate chondrocytes during development and after bone fracture (6, 7). IL-17B is up-regulated in arthritic cartilage where it exacerbates the severity of disease (8). It is also up-regulated in aggressive breast cancer cell lines, and its exaggerated signaling through IL-17 RB promotes tumorigenicity (9). It inhibits the adhesion of vascular endothelial cells to Matrigel and enhances their migration (10).

References
  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  4. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
  5. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
  6. Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89.
  7. You, Z. et al. (2005) Biochem. Biophys. Res. Commun. 326:624.
  8. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
  9. Huang, C.-K. et al. (2013) Oncogene July 15 Epub.
  10. Sanders, A.J. et al. (2010) J. Oncol. 2010:817375.
Long Name
Interleukin 17B
Entrez Gene IDs
27190 (Human); 56069 (Mouse)
Alternate Names
Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20

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