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Recombinant Human IL-18 BPa Fc Chimera Protein, CF

R&D Systems | Catalog # 119-BP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human IL-18 BPa Fc Chimera Protein (119-BP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAD17190

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-18 BPa Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-18 BPa protein
Human IL-18 Bpa
(Thr29-Gly192)
Accession # AAD17190		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr29

Predicted Molecular Mass

44.2 kDa (monomer)

SDS-PAGE

60-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells.
The ED50 for this effect is 0.02‑0.12 µg/mL in the presence of 40 ng/mL of recombinant human IL-18.

Reviewed Applications

Read 1 review rated 4 using 119-BP in the following applications:

Formulation, Preparation, and Storage

119-BP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

References

  1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
  2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
  3. Calderara, S. et al. (2001) Virology 279:22.

Long Name

Interleukin 18 Binding Protein Isoform a

Alternate Names

IL18 BPa, IL18BP

Entrez Gene IDs

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Gene Symbol

IL18BP

UniProt

AAD17190

Additional IL-18 BPa Products

Product Documents for Recombinant Human IL-18 BPa Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IL-18 BPa Fc Chimera Protein, CF

For research use only

Related Research Areas

IL-1 Family

Citations for Recombinant Human IL-18 BPa Fc Chimera Protein, CF

Customer Reviews for Recombinant Human IL-18 BPa Fc Chimera Protein, CF (1)

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  • Recombinant Human IL-18 BPa Fc Chimera Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/09/2017
    MAB1192 was used as the capture and AF119 was used as the detection antibody for the sandwich ELISA for IL-18PB. The immunoassay standard was 119-BP. Assay had sensitivity of ~10pg/ml.
    Recombinant Human IL-18 BPa Fc Chimera Protein, CF 119-BP

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