Recombinant Human IL-18 BPa Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
119-BP-100
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human IL-18 BPa Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.02‑0.12 µg/mL in the presence of 40 ng/mL of recombinant human IL-18.
Source
Mouse myeloma cell line, NS0-derived human IL-18 BPa protein
Human IL-18 Bpa
(Thr29-Gly192)
Accession # AAD17190
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44.2 kDa (monomer)
SDS-PAGE
60-80 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

119-BP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

References
  1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
  2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
  3. Calderara, S. et al. (2001) Virology 279:22.
Long Name
Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs
10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names
IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa

Citations for Recombinant Human IL-18 BPa Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Interleukin-18 diagnostically distinguishes and pathogenically promotes human and murine macrophage activation syndrome
    Authors: ES Weiss, C Girard-Guy, D Holzinger, AA de Jesus, Z Tariq, J Picarsic, EJ Schiffrin, D Foell, AA Grom, S Ammann, S Ehl, T Hoshino, R Goldbach-M, C Gabay, SW Canna
    Blood, 2018;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: Multiplex Standard
  2. IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
    Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
    Respir. Res., 2017;18(1):159.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Characterization of the Inflammasome in Human Kupffer Cells in Response to Synthetic Agonists and Pathogens
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Pro-inflammatory interleukin-18 increases Alzheimer's disease-associated amyloid-beta production in human neuron-like cells.
    J Neuroinflammation, 2012;9(0):199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.
    Authors: Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2009;297(2):H874-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Yaba monkey tumor virus encodes a functional inhibitor of interleukin-18.
    Authors: Nazarian SH, Rahman MM, Werden SJ, Villeneuve D, Meng X, Brunetti C, Valeriano C, Wong C, Singh R, Barrett JW, Xiang Y, McFadden G
    J. Virol., 2007;82(1):522-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  7. Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
    Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
    Ann. Neurol., 2005;57(1):50-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
    Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
    J. Immunol., 2003;170(11):5571-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay

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Recombinant Human IL-18 BPa Fc Chimera Protein, CF
By Anonymous on 11/09/2017
Application: Immunoassay Standard

MAB1192 was used as the capture and AF119 was used as the detection antibody for the sandwich ELISA for IL-18PB. The immunoassay standard was 119-BP. Assay had sensitivity of ~10pg/ml.