Recombinant Human mGluR3 Protein, CF
Recombinant Human mGluR3 Protein, CF Summary
Asp25-Ser507, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). The Group II receptors comprise mGluR2 and mGluR3, which share high sequence homology. mGluR3 is a presynaptic receptor expressed on both neurons and glia, and its activation regulates cAMP accumulation (1). Mature human mGluR3 is 857 amino acids (aa) in length and aa 2-507 represents the N-terminal ECD (4), with the apparent molecular mass approximately 66 kDa in SDS-PAGE under reducing conditions. Over aa 2-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3.
- Conn PJ, et al. (1997) Annu Rev Pharmacol Toxicol 37:205.
- Pin JP, et al. (1995) Neuropharmacology. 34:1.
- Niswender CM, et al. (2010) Annu Rev Pharmacol Toxicol 50:295.
- Monn, J.A. et al. (2015) J Med Chem 58:7526.
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