Recombinant Human MINDY3 Protein, CF

R&D Systems | Catalog # E-621

R&D Systems
Discontinued Product
E-621 has been discontinued. View all MINDY3 products.

Key Product Details

Source

E. coli

Applications

Bioactivity
View all MINDY3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MINDY3 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

50 kDa

Activity

Recombinant Human MINDY-3 is a Ubiquitin-specific deconjugating enzyme that cleaves K48-linked polyubiquitin chains.

Reviewed Applications

Read 1 review rated 5 using E-621 in the following applications:

Formulation, Preparation, and Storage

E-621
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: MINDY3

MINDY-3/FAM188A is a member of a newly characterized cysteine protease family of deubiquitinases ("DUBs").  In humans, the MINDY (MIU containing novel DUB family) family has at least four members, with an additional three being MINDY-1/FAM63A, MINDY-2/FAM63B and MINDY-4/FAM188B.  The catalytic domain of MINDY DUBs adopts a conformation that is distinct from other known DUB classes, and these enzymes are highly selective at cleaving K48-linked polyubiquitin chains.  With a predicted molecular weight of 50 kDa, human MINDY-3 is 95% identical to both its murine and rat orthologs.  This recombinant protein is untagged.

References

  1. Abdul-Rehman S.A. et al. (2016)  Mol. Cell 63: 146
  2. Kristariyanto Y.A. et al. (2017) EMBO Reports  DOI 10.15252/embr.201643205

Long Name

Ubiquitin Carboxyl-Terminal Hydrolase MINDY-3

Alternate Names

C10orf97, FAM188A, MSTP126, My042

Entrez Gene IDs

80013 (Human)

Gene Symbol

MINDY3

Additional MINDY3 Products

Product Documents for Recombinant Human MINDY3 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human MINDY3 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human MINDY3 Protein, CF

Customer Reviews for Recombinant Human MINDY3 Protein, CF (1)

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/21/2021

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