Recombinant Human NKG2D Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1299-NK-050
Product Details
Citations (24)
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Recombinant Human NKG2D Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind its ligand, rhMICA/Fc Chimera in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived human NKG2D/CD314 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human NKG2D
(Phe78-Val216)
Accession # P26718
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1299-NK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

References
  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.rndsystems.com
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Citations for Recombinant Human NKG2D Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses
    Authors: J Prévost, J Richard, H Medjahed, A Alexander, J Jones, JC Kappes, C Ochsenbaue, A Finzi
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells
    Authors: Y Han, W Xie, DG Song, DJ Powell
    J Hematol Oncol, 2018;11(1):92.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  3. Phenotypic characterization and anticancer capacity of CD8+ cytokine-induced killer cells after antigen-induced expansion
    Authors: J Liu, L Wang, Y Wang, W Zhang, Y Cao
    PLoS ONE, 2017;12(4):e0175704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D.
    Authors: Eggink L, Spyroulias G, Jones N, Hanson C, Hoober J
    PLoS ONE, 2015;10(6):e0130532.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Isothermal Microcalorimetry
  5. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
    Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
    PLoS ONE, 2015;10(5):e0125544.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor activity against colon carcinoma.
    Authors: Rothe A, Jachimowicz R, Borchmann S, Madlener M, Kessler J, Reiners K, Sauer M, Hansen H, Ullrich R, Chatterjee S, Borchmann P, Yazaki P, Koslowsky T, Engert A, Heukamp L, Hallek M, von Strandmann E
    Int J Cancer, 2014;134(12):2829-40.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  7. Metalloprotease-mediated tumor cell shedding of B7-H6, the ligand of the natural killer cell-activating receptor NKp30.
    Authors: Schlecker E, Fiegler N, Arnold A, Altevogt P, Rose-John S, Moldenhauer G, Sucker A, Paschen A, von Strandmann E, Textor S, Cerwenka A
    Cancer Res, 2014;74(13):3429-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
    Authors: Mellergaard M, Skovbakke S, Schneider C, Lauridsen F, Andresen L, Jensen H, Skov S
    J Biol Chem, 2014;289(29):20078-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
    Authors: Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming
    Sci Rep, 2014;4(0):6138.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity.
    Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N
    Leukemia, 2014;28(6):1316-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer.
    Authors: Cho, Hanbyoul, Chung, Joon-Yon, Kim, Sunghoon, Braunschweig, Till, Kang, Tae Heun, Kim, Jennie, Chung, Eun Joo, Hewitt, Stephen, Kim, Jae-Hoon
    BMC Cancer, 2014;14(0):957.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
    Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
    J Immunol, 2014;193(4):1654-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
    Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
    J Exp Med, 2013;210(12):2675-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients.
    Authors: Reiners K, Kessler J, Sauer M, Rothe A, Hansen H, Reusch U, Hucke C, Kohl U, Durkop H, Engert A, von Strandmann E
    Mol Ther, 2013;21(4):895-903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Human cytomegalovirus infection elicits new decidual natural killer cell effector functions.
    Authors: Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N
    PLoS Pathog, 2013;9(4):e1003257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells.
    Authors: Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J
    J. Clin. Invest., 2012;122(7):2369-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008;384(5):1143-56.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  19. Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells.
    Authors: Banerjee P, Feuer G, Barker E
    J. Virol., 2007;81(18):9707-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. The requirement for DNAM-1, NKG2D, and NKp46 in the natural killer cell-mediated killing of myeloma cells.
    Authors: El-Sherbiny YM, Meade JL, Holmes TD, McGonagle D, Mackie SL, Morgan AW, Cook G, Feyler S, Richards SJ, Davies FE, Morgan GJ, Cook GP
    Cancer Res., 2007;67(18):8444-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
    Authors: Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, Barker E
    Blood, 2007;110(4):1207-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo.
    Authors: von Strandmann EP, Hansen HP, Reiners KS, Schnell R, Borchmann P, Merkert S, Simhadri VR, Draube A, Reiser M, Purr I, Hallek M, Engert A
    Blood, 2006;107(5):1955-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
    Authors: Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
    Cancer Res., 2005;65(23):11136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
    Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
    Cancer Res., 2005;65(14):6409-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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