Prostaglandin E Synthase 3 (also known as Cytosolic prostaglandin E2 synthase, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, or p23) is a glutathione-dependent enzyme found in the cyclooxygenase-1-mediated PGE2 biosynthetic pathway. In addition to its catalytic activity in the prostaglandin biosynthesis pathway, P23 serves as a co-chaperone to HSP90 in various biological functions. The p23/HSP90 complex is required for efficient telomerase assembly in vitro and in vivo. It has also been demonstrated that p23 and HSP90 localize to genomic response elements in a hormone-dependent manner, and may promote disassembly of transcriptional regulatory complexes in response to changes in cellular signaling pathways. This recombinant protein may be used in conjunction with HSP90 alpha (AP-160) in various in vitro protein refolding assays.
Recombinant Human p23/PTGES3 Protein, CF
R&D Systems | Catalog # AP-170
Key Product Details
Source
Accession Number
Applications
Product Specifications
Source
Accession # Q15185
Purity
Predicted Molecular Mass
Activity
p23/PTGES3 can serve as a molecular co-chaperone for HSP90. Reaction conditions will need to be optimized for each specific application. For in vitro reactions containing both HSP90 alpha /HSP90AA1 (Catalog # AP-160) and p23/PTGES3, we recommend an initial HSP90 concentration of 2-3 μM and an equimolar concentration of p23.
Formulation, Preparation, and Storage
AP-170
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: p23/PTGES3
References
- Freeman B.C. & Yamamoto K.R. (2002) Science 296: 2232-2235.
- Forsythe H.L., et al. (2001) J. Biol. Chem. 276: 15571-15574.
- Patwardhan C.A., et al. (2013) J. Biol. Chem. 288: 7313-7325.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional p23/PTGES3 Products
Product Documents for Recombinant Human p23/PTGES3 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human p23/PTGES3 Protein, CF
For research use only
Citations for Recombinant Human p23/PTGES3 Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Human p23/PTGES3 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human p23/PTGES3 Protein, CF and earn rewards!
Have you used Recombinant Human p23/PTGES3 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review