Recombinant Human p23/PTGES3 Protein, CF
Recombinant Human p23/PTGES3 Protein, CF Summary
p23/PTGES3 can serve as a molecular co-chaperone for HSP90. Reaction conditions will need to be optimized for each specific application. For in vitro reactions containing both HSP90 alpha /HSP90AA1 (Catalog # AP-160) and p23/PTGES3, we recommend an initial HSP90 concentration of 2-3 μM and an equimolar concentration of p23.
Accession # Q15185
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Prostaglandin E Synthase 3 (also known as Cytosolic prostaglandin E2 synthase, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, or p23) is a glutathione-dependent enzyme found in the cyclooxygenase-1-mediated PGE2 biosynthetic pathway. In addition to its catalytic activity in the prostaglandin biosynthesis pathway, P23 serves as a co-chaperone to HSP90 in various biological functions. The p23/HSP90 complex is required for efficient telomerase assembly in vitro and in vivo. It has also been demonstrated that p23 and HSP90 localize to genomic response elements in a hormone-dependent manner, and may promote disassembly of transcriptional regulatory complexes in response to changes in cellular signaling pathways. This recombinant protein may be used in conjunction with HSP90 alpha (AP-160) in various in vitro protein refolding assays.
- Freeman B.C. & Yamamoto K.R. (2002) Science 296: 2232-2235.
- Forsythe H.L., et al. (2001) J. Biol. Chem. 276: 15571-15574.
- Patwardhan C.A., et al. (2013) J. Biol. Chem. 288: 7313-7325.
Citations for Recombinant Human p23/PTGES3 Protein, CF
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The STING ligand cGAMP potentiates the efficacy of vaccine-induced CD8+ T cells
Authors: A Gutjahr, L Papagno, F Nicoli, T Kanuma, N Kuse, MP Cabral-Pic, N Rochereau, E Gostick, T Lioux, E Perouzel, DA Price, M Takiguchi, B Verrier, T Yamamoto, S Paul, V Appay
JCI Insight, 2019;4(7):.
Sample Types: Whole Cells
Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta
Authors: S Haider, G Meinhardt, L Saleh, V Kunihs, M Gamperl, U Kaindl, A Ellinger, TR Burkard, C Fiala, J Pollheimer, S Mendjan, PA Latos, M Knöfler
Stem Cell Reports, 2018;0(0):.
Sample Types: Whole Cells
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