Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF

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Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. Immobilized recombinant human (rh) PD-L2 at 1 µg/mL (100 µL/well) can bind  rhPD-1 with a linear range of 7.5-500 ng/mL.
Mouse myeloma cell line, NS0-derived human PD-L2/B7-DC protein
Human PD-L2
Accession # Q9BQ51.2
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49 kDa (monomer)
70-80 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. Alternative splicing generates additional isoforms that lack the second Ig-like domain and may be substituted and truncated following the first Ig-like domain (4). PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3, 5, 6). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (7-11). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 12, 13). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (9, 11).

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
  4. He, X.-H. et al. (2004) Acta Biochim. Biophys. Sinica 36:284.
  5. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  6. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  7. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  8. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  9. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  10. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  11. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  12. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  13. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  14. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
Long Name
Programmed Death-1 Ligand-2
Entrez Gene IDs
80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
Alternate Names
B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2

Citations for Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Inherited PD-1 deficiency underlies tuberculosis and autoimmunity in a child
    Authors: M Ogishi, R Yang, C Aytekin, D Langlais, M Bourgey, T Khan, FA Ali, M Rahman, OM Delmonte, M Chrabieh, P Zhang, C Gruber, SJ Pelham, AN Spaan, J Rosain, WT Lei, S Drutman, MD Hellmann, MK Callahan, M Adamow, P Wong, JD Wolchok, G Rao, CS Ma, Y Nakajima, T Yaguchi, K Chamoto, SC Williams, JF Emile, F Rozenberg, MS Glickman, F Rapaport, G Kerner, G Allington, I Tezcan, D Cagdas, FO Hosnut, F Dogu, A Ikinciogul, VK Rao, L Kainulaine, V Béziat, J Bustamante, S Vilarinho, RP Lifton, B Boisson, L Abel, D Bogunovic, N Marr, LD Notarangel, SG Tangye, T Honjo, P Gros, S Boisson-Du, JL Casanova
    Nature Medicine, 2021;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Characterization of the anti-PD-1 antibody REGN2810 and its antitumor activity in human PD-1 knock-in mice
    Authors: E Burova, A Hermann, J Waite, T Potocky, V Lai, S Hong, M Liu, O Allbritton, A Woodruff, Q Wu, A D'Orvillie, E Garnova, A Rafique, W Poueymirou, J Martin, T Huang, D Skokos, J Kantrowitz, J Popke, M Mohrs, D MacDonald, E Ioffe, W Olson, I Lowy, A Murphy, G Thurston
    Mol. Cancer Ther, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  3. The coreceptor programmed death 1 inhibits T-cell adhesion by regulating Rap1.
    Authors: Azoulay-Alfaguter, Inbar, Strazza, Marianne, Pedoeem, Ariel, Mor, Adam
    J Allergy Clin Immunol, 2015;135(2):564-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers.
    Authors: Steidl C, Shah SP, Woolcock BW
    Nature, 2011;471(7338):377-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF
By Anonymous on 11/22/2019
Application: Binding assay/Protein-protein interaction

Cells expressing a negative control (left) or hPD-1 mCherry (right) were treated with 0.25 ug of hPD-L2 hIgG1. Cells were analyzed by flow cytometry with binding detected using an anti-human 488 secondary antibody.

Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF
By Casey Keyes on 11/30/2017
Application: Immunoassay Standard

Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF
By Jenna Nguyen on 11/03/2017
Application: Binding assay/Protein-protein interaction