RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.
Recombinant Human RNF4 Protein, CF
R&D Systems | Catalog # E3-210
Discontinued Product
E3-210 has been discontinued.
View all RNF4 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human RNF4 protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
22 kDa
Activity
Recombinant Human RNF4 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human RNF4 concentration of 100-500 nM.
Formulation, Preparation, and Storage
E3-210
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 5 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: RNF4
References
-
Geoffroy, M-C et al. (2010) Mol. Bio. Cell 21: 4227
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Tathum, M.H. et al. (2008) Nat. Cell Bio. 10: 538
Long Name
Ring Finger Protein 4
Alternate Names
RES4-26, SLX5, SNURF
Entrez Gene IDs
6047 (Human)
Gene Symbol
RNF4
UniProt
Additional RNF4 Products
Product Documents for Recombinant Human RNF4 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human RNF4 Protein, CF
For research use only
Citations for Recombinant Human RNF4 Protein, CF
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Associated Pathways
