Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate

R&D Systems | Catalog # ATM1968

Fc Chimera
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate (ATM1968)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

CAA42006.1

Structure / Form

Disulfide-linked homodimer, labeled with Atto 647N via amines
Excitation Wavelength: 647 nm
Emission Wavelenght: 667 nm

Conjugate

ATTO 647N (Excitation= 647 nm, Emission= 667 nm)

Applications

Bioactivity
View all Siglec-2/CD22 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Siglec-2/CD22 protein
Human Siglec-2/CD22
(Asp20-Arg687)
Accession # CAA42006.1		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

101.9 kDa (monomer)

SDS-PAGE

114-126 kDa, under reducing conditions

Activity

Measured by flow cytometry for its ability to bind anti-Human Siglec-2/CD22 Monoclonal Antibody conjugated fluorescent beads.

Scientific Data Images for Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate

Detection of Human Siglec-2/CD22 Antibody with Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate by Flow Cytometry.

Fluorescent beads conjugated to anti-Human Siglec 2/CD22 Monoclonal Antibody (MAB1968) were stained with (A) Recombinant Human Siglec 2/CD22 Protein, Atto 647N Conjugate (Catalog # ATM1968) or (B) unstained.

Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate SDS-PAGE.

2 μg/lane of Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate (Catalog # ATM1968) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-126 kDa and 228-252 kDa, respectively.

Formulation, Preparation, and Storage

ATM1968
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Siglec-2/CD22

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the identified Siglecs 5 to 11 (1 ‑ 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Human Siglec-2, also known as B‑cell antigen CD22 or B‑lymphocyte cell adhesion molecule (BL-CAM), is a B‑cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. The predominant Siglec-2/CD22 beta encodes an 847 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with 4 immunoreceptor tyrosine-based inhibition motifs (ITIMs) (4). The variant Siglec-2/CD22 alpha encodes a 647 aa polypeptide missing two Ig-like C2-type domains and has a truncated (23 aa) cytoplasmic tail (5). Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis (9). Besides its role as an adhesion molecule, Siglec-2/CD22 is a coreceptor that physically interacts with B‑cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation. It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2/CD22 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling (2, 7, 8).

References

  1. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  4. Wilson, G.L et al. (1991) J. Exp. Med. 173:137.
  5. Stamenkovic, I. and B. Seed (1990) Nature 345:74.
  6. Kelm, S. et al. (1994) Current Bio. 4:965.
  7. Ravetch, J.V. and L.L. Lanier (2000) Science 290:84.
  8. Wienands, Y.J. et al. (1999) J. Biol. Chem. 274:18769.
  9. Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Entrez Gene IDs

933 (Human); 12483 (Mouse); 308501 (Rat); 102130968 (Cynomolgus Monkey)

Gene Symbol

CD22

UniProt

CAA42006.1

Additional Siglec-2/CD22 Products

Product Documents for Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate

Certificate of Analysis

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Product Specific Notices for Recombinant Human Siglec-2/CD22 Protein, Atto 647N Conjugate

For research use only

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