Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF Summary
Cys24-Asn573, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human TGF- beta 1 (Catalog # 240-B) is used at 2 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT-like 2 (Catalog # 10156-VN) binds with an ED50of 0.6-3.6 µg/mL.
2 μg/lane of Recombinant Human Vasorin/SLIT-like 2 His-tag (Catalog # 10156-VN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-95 kDa.
Background: Vasorin/SLIT-like 2
Vasorin, also known as SLIT-like 2, is an approximately 110-kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95-kDa soluble form of Vasorin can be shed from the cell surface by ADAM17-mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta-induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature human Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type III domain, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (1). Within the ECD, human Vasorin shares 85% and 86% aa sequence identity with mouse and rat Vasorin, respectively.
- Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
- Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
- Malapeira, J. et al. (2011) Oncogene 30:1912.
- Li, S. et al. (2015) Oncotarget 6:10045.
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