Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10156-VN-050
Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF Binding Activity
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Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human TGF‑ beta 1 (Catalog # 240-B) is used at 2 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT‑like 2 (Catalog # 10156-VN) binds with an ED50 of 0.6-3.6 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Vasorin/SLIT-like 2 protein
Cys24-Asn573, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys24
Predicted Molecular Mass
60 kDa
SDS-PAGE
83-95 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10156-VN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human TGF- beta 1  (Catalog # 240-B) is usedat 2 µg/mL (100 µL/well), Recombinant Human  Vasorin/SLIT‑like 2 (Catalog # 10156-VN) bindswith an ED50 of 0.6‑3.6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Vasorin/SLIT-like 2 His-tag(Catalog # 10156-VN) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 83-95 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vasorin/SLIT-like 2

Vasorin, also known as SLIT-like 2, is an approximately 110-kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95-kDa soluble form of Vasorin can be shed from the cell surface by ADAM17-mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta-induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature human Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type III domain, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (1). Within the ECD, human Vasorin shares 85% and 86% aa sequence identity with mouse and rat Vasorin, respectively.

References
  1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
  2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
  3. Malapeira, J. et al. (2011) Oncogene 30:1912.
  4. Li, S. et al. (2015) Oncotarget 6:10045.
Entrez Gene IDs
114990 (Human); 246154 (Mouse)
Alternate Names
Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; Vasorin

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