Recombinant Human TGF-beta 1 Protein

Name: Recombinant Human TGF-beta 1 Protein
Brand: R&D Systems
Rating: 4.6 (13 reviews)

A New TGF-beta 1 is Available! The new protein is Hek293 expressed!
New Product 7754-BH

(361 citations)

(13 Reviews)

Bioactivity Recombinant Human TGF-beta 1 Protein Bioactivity

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SDS-PAGE Recombinant Human TGF-beta 1 Protein SDS-PAGE

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Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED₅₀ for this effect is 0.04-0.2 ng/mL.
The specific activity of Recombinant Human TGF-beta 1 is approximately 2.5 x 10⁴ U/μg, which is calibrated against human TGF‑ beta 1 Standard (NIBSC code: 89/514).
Source
Chinese Hamster Ovary cell line, CHO-derived Ala279-Ser390
Accession #

P01137
N-terminal Sequence
Analysis
Ala279
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.8 kDa (monomer)
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

240-B		240-B/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.		Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.		Reconstitution Reconstitute 2 µg vials at 20 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.		Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.		Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity click image to view larger	Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL.
SDS-PAGE click image to view larger	1 ug/lane of Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Bioactivity click image to view larger	Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh)TGF-beta 1 (Catalog # 240-B). Control cells were cultured without rhTGF-beta 1. EMT induction was confirmed at 48 h by flow cytometric staining for E-Cadherin (filled; Catalog # FAB18381P), an epithelial cell marker, or an isotype control(Catalog # 240-B). TGF-beta 1 decreased the expression of E-Cadherin.

Background: TGF-beta 1

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References:
1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
5. Lin, J.S. et al. (2006) Reproduction 132:179.
6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
8. Derynck, R. et al. (1985) Nature 316:701.
9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name:
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names:
CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

361 Citations: Showing 1 - 10
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Species	Applications	Sample Type
A ZIP6-ZIP10 heteromer controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition Authors: D Brethour, M Mehrabian, D Williams, X Wang, F Ghodrati, S Ehsani, EA Rubie, JR Woodgett, J Sevalle, Z Xi, E Rogaeva, G Schmitt-Ul Sci Rep, 2017;7(0):40313. Species: Human Sample Type: Whole Cells Application: Bioassay Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling Authors: D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó Kidney Blood Press. Res, 2017;42(1):16-32. Species: Human Sample Type: Whole Cells Application: Bioassay Armc5 deletion causes developmental defects and compromises T-cell immune responses Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu Nat Commun, 2017;8(0):13834. Species: Mouse Sample Type: Whole Cells Application: Bioassay Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis Authors: MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E524-E533. Species: Mouse Sample Type: Whole Cells Application: Bioassay Fibronectin-guided migration of carcinoma collectives Authors: S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg Nat Commun, 2017;8(0):14105. Species: Human Sample Type: Whole Cells Application: Bioassay Prion Protein Deficiency Causes Diverse Proteome Shifts in Cell Models That Escape Detection in Brain Tissue Authors: Mohadeseh Mehrabian PLoS ONE, 2016;11(6):e0156779. Species: Mouse Sample Type: Whole Cells Application: Bioassay CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT Nat Commun, 2016;7(0):13362. Species: Human Sample Type: Whole Cells Application: Bioassay Integrative functional genomics identifies regulatory mechanisms at coronary artery disease loci Nat Commun, 2016;7(0):12092. Ablation of the N-type calcium channel ameliorates diabetic nephropathy with improved glycemic control and reduced blood pressure Authors: Shoko Ohno Sci Rep, 2016;6(0):27192. Species: Human Sample Type: Whole Cells Application: Bioassay Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors Clin Cancer Res, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Bioassay Hepatitis C virus depends on E-cadherin as an entry factor and regulates its expression in epithelial-to-mesenchymal transition Authors: Qisheng Li Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Bioassay CD1c-Expression by Monocytes - Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits Authors: Martine Schrøder PLoS ONE, 2016;11(6):e0157387. Species: Human Sample Type: Whole Cells Application: Bioassay MAP3K19 Is a Novel Regulator of TGF-? Signaling That Impacts Bleomycin-Induced Lung Injury and Pulmonary Fibrosis PLoS ONE, 2016;11(5):e0154874. Species: Human Sample Type: Whole Cells Application: Bioassay 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats PLoS ONE, 2016;11(8):e0161782. Species: Rat Sample Type: Whole Cells Application: Bioassay Context-Specific Function of S6K2 in Th Cell Differentiation J Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Bioassay Trichostatin A Inhibits Epithelial Mesenchymal Transition Induced by TGF-?1 in Airway Epithelium PLoS ONE, 2016;11(8):e0162058. Species: Human Sample Type: Whole Cells Application: Bioassay Tissue factor induced by epithelial-mesenchymal transition triggers a pro-coagulant state that drives metastasis of circulating tumor cells Cancer Res, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Bioassay Interleukin-22 ameliorates liver fibrosis through miR-200a/beta-catenin Sci Rep, 2016;6(0):36436. Species: Rat Sample Type: Whole Cells Application: Bioassay Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching Sci Rep, 2016;6(0):37215. Species: Mouse Sample Type: Whole Cells Application: Bioassay Cell-cell contact and matrix adhesion promote ?SMA expression during TGF?1-induced epithelial-myofibroblast transition via Notch and MRTF-A Sci Rep, 2016;6(0):26226. Species: Mouse Sample Type: Whole Cells Application: Bioassay Developmental Reprogramming in Mesenchymal Stromal Cells of Human Subjects with Idiopathic Pulmonary Fibrosis Sci Rep, 2016;6(0):37445. Species: Human Sample Type: Whole Cells Application: Bioassay IL-10 production in murine IgM(+) CD138(hi) cells is driven by Blimp1 and downregulated in class-switched cells Eur. J. Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Bioassay Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases Cell Death Dis, 2016;7(12):e2565. Species: Human Sample Type: Whole Cells Application: Bioassay p(70S6K1) in the TORC1 pathway is essential for the differentiation of Th17 Cells, but not Th1, Th2, or Treg cells in mice. Authors: Sasaki C, Chen G, Munk R, Eitan E, Martindale J, Longo D, Ghosh P Eur J Immunol, 2016;46(1):212-22. Species: Mouse Sample Type: Whole Cells Application: Bioassay Patient-Specific Screening Using High-Grade Glioma Explants to Determine Potential Radiosensitization by a TGF-? Small Molecule Inhibitor Neoplasia, 2016;18(12):795-805. Species: Human Sample Type: Whole Cells Application: Bioassay Transient RUNX1 Expression during Early Mesendodermal Differentiation ofï¿½hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling Stem Cell Reports, 2016;7(5):884-896. Species: Human Sample Type: Whole Cells Application: Bioassay EZH2 enhances the differentiation of fibroblasts into myofibroblasts in idiopathic pulmonary fibrosis Physiol Rep, 2016;4(17):. Species: Human Sample Type: Whole Cells Application: Bioassay Loss of TRPV4 Function Suppresses Inflammatory Fibrosis Induced by Alkali-Burning Mouse Corneas PLoS ONE, 2016;11(12):e0167200. Species: Mouse Sample Type: Whole Cells Application: Bioassay Fscn1 is required for the trafficking of TGF-? family type I receptors during endoderm formation Nat Commun, 2016;7(0):12603. Species: Mouse Sample Type: Whole Cells Application: Bioassay Autonomous and Non-autonomous Defects Underlie Hypertrophic Cardiomyopathy in BRAF-Mutant hiPSC-Derived Cardiomyocytes Stem Cell Reports, 2016;0(0):. Species: Human Sample Type: protein Application: ELISA Standard Differentiation defect in neural crest-derived smooth muscle cells in patients with aortopathy associated with bicuspid aortic valves EBioMedicine, 2016;10(0):282-90. Species: Human Sample Type: Whole Cells Application: Bioassay Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity. Authors: Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J Nat Med, 2015;21(5):530-6. Species: Human Sample Type: Whole Cells Application: Bioassay Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells. Authors: Woltje K, Jabs M, Fischer A PLoS ONE, 2015;10(3):e0120547. Species: Human Sample Type: Whole Cells Application: Bioassay BMP9 Crosstalk with the Hippo Pathway Regulates Endothelial Cell Matricellular and Chemokine Responses. Authors: Young K, Tweedie E, Conley B, Ames J, FitzSimons M, Brooks P, Liaw L, Vary C PLoS ONE, 2015;10(4):e0122892. Species: Human Sample Type: Whole Cells Application: Bioassay Suppressor of cytokine signaling 1 is a positive regulator of TGF-beta-induced prostaglandin production in human follicular dendritic cell-like cells. Authors: Cho W, Kim Y, Kim J, Park S, Park D, Kim B, Jeoung D, Kim Y, Choe J J Immunol, 2015;194(9):4287-97. Species: Human Sample Type: Whole Cells Application: Bioassay The anti-fibrotic effects of epigallocatechin-3-gallate in bile duct-ligated cholestatic rats and human hepatic stellate LX-2 cells are mediated by the PI3K/Akt/Smad pathway. Authors: Yu D, Zhang C, Zhao S, Zhang S, Zhang H, Cai S, Shao R, He H Acta Pharmacol Sin, 2015;36(4):473-82. Species: Human Sample Type: Whole Cells Application: Bioassay Mitochondrial SOD2 regulates epithelial-mesenchymal transition and cell populations defined by differential CD44 expression. Authors: Kinugasa H, Whelan K, Tanaka K, Natsuizaka M, Long A, Guo A, Chang S, Kagawa S, Srinivasan S, Guha M, Yamamoto K, St Clair D, Avadhani N, Diehl J, Nakagawa H Oncogene, 2015;34(41):5229-39. Species: Human Sample Type: Whole Cells Application: Bioassay TGFbeta-induced phosphorylation of Par6 promotes migration and invasion in prostate cancer cells. Authors: Mu Y, Zang G, Engstrom U, Busch C, Landstrom M Br J Cancer, 2015;112(7):1223-31. Species: Human Sample Type: Whole Cells Application: Bioassay MiR-133 modulates TGF-ÃŽÂ²1-induced bladder smooth muscle cell hypertrophic and fibrotic response: implication for a role of microRNA in bladder wall remodeling caused by bladder outlet obstruction. Cell Signal, 2015;27(2):215-27. Species: Human Sample Type: Whole Cells Application: Bioassay Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9. Authors: Rojas-Fernandez A, Herhaus L, Macartney T, Lachaud C, Hay R, Sapkota G Sci Rep, 2015;5(0):9811. Species: Human Sample Type: Whole Cells Application: Bioassay Role and species-specific expression of colon T cell homing receptor GPR15 in colitis. Authors: Nguyen L, Pan J, Dinh T, Hadeiba H, O'Hara E, Ebtikar A, Hertweck A, Gokmen M, Lord G, Jenner R, Butcher E, Habtezion A Nat Immunol, 2015;16(2):207-13. Species: Mouse Sample Type: Whole Cells Application: Bioassay Dietary n-3 PUFA Protects Mice from Con A Induced Liver Injury by Modulating Regulatory T Cells and PPAR-gamma Expression. Authors: Lian M, Luo W, Sui Y, Li Z, Hua J PLoS ONE, 2015;10(7):e0132741. Species: Human Sample Type: Whole Cells Application: Bioassay Identification and Characterization of CXCR4-Positive Gastric Cancer Stem Cells. Authors: Fujita T, Chiwaki F, Takahashi R, Aoyagi K, Yanagihara K, Nishimura T, Tamaoki M, Komatsu M, Komatsuzaki R, Matsusaki K, Ichikawa H, Sakamoto H, Yamada Y, Fukagawa T, Katai H, Konno H, Ochiya T, Yoshida T, Sasaki H PLoS ONE, 2015;10(6):e0130808. Species: Human Sample Type: Whole Cells Application: Bioassay Mesenchymal stem cells from patients with rheumatoid arthritis display impaired function in inhibiting Th17 cells. Authors: Sun, Yue, Deng, Wei, Geng, Linyu, Zhang, Lu, Liu, Rui, Chen, Weiwei, Yao, Genhong, Zhang, Huayong, Feng, Xuebing, Gao, Xiang, Sun, Lingyun J Immunol Res, 2015;2015(0):284215. Species: Human Sample Type: Whole Cells Application: Bioassay Role of a Novel Human Leukocyte Antigen-DQA101:02;DRB115:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease. Authors: Kaushansky N, Eisenstein M, Boura-Halfon S, Hansen B, Nielsen C, Milo R, Zeilig G, Lassmann H, Altmann D, Ben-Nun A J Biol Chem, 2015;290(24):15260-78. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Standard Salinomycin and other polyether ionophores are a new class of antiscarring agent. Authors: Woeller C, O'Loughlin C, Roztocil E, Feldon S, Phipps R J Biol Chem, 2015;290(6):3563-75. Species: Human Sample Type: Whole Cells Application: Bioassay JNK-dependent gene regulatory circuitry governs mesenchymal fate. Authors: Sahu S, Garding A, Tiwari N, Thakurela S, Toedling J, Gebhard S, Ortega F, Schmarowski N, Berninger B, Nitsch R, Schmidt M, Tiwari V EMBO J, 2015;34(16):2162-81. Species: Mouse Sample Type: Whole Cells Application: Bioassay The DNA-binding inhibitor Id3 regulates IL-9 production in CD4(+) T cells. Authors: Nakatsukasa H, Zhang D, Maruyama T, Chen H, Cui K, Ishikawa M, Deng L, Zanvit P, Tu E, Jin W, Abbatiello B, Goldberg N, Chen Q, Sun L, Zhao K, Chen W Nat Immunol, 2015;16(10):1077-84. Species: Mouse Sample Type: Whole Cells Application: Bioassay Lipoxin A4 Attenuates Constitutive and TGF-beta1-Dependent Profibrotic Activity in Human Lung Myofibroblasts. Authors: Roach K, Feghali-Bostwick C, Amrani Y, Bradding P J Immunol, 2015;195(6):2852-60. Species: Human Sample Type: Whole Cells Application: Bioassay TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J Oncogene, 2015;35(6):748-60. Species: Mouse Sample Type: Whole Cells Application: Bioassay Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination. Authors: Masani S, Han L, Meek K, Yu K Proc Natl Acad Sci U S A, 2015;113(5):1261-6. Species: Mouse Sample Type: Whole Cells Application: Bioassay Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Authors: Lu G, Duan J, Shu S, Wang X, Gao L, Guo J, Zhang Y Proc Natl Acad Sci U S A, 2015;113(5):1256-60. Species: Mouse Sample Type: Whole Cells Application: Bioassay Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne Muscular Dystrophy. Authors: Pessina P, Cabrera D, Morales M, Riquelme C, Gutierrez J, Serrano A, Brandan E, Munoz-Canoves P Skelet Muscle, 2015;4(0):7. Species: Mouse Sample Type: In Vivo Application: In Vivo The Prion Protein Controls Polysialylation of Neural Cell Adhesion Molecule 1 during Cellular Morphogenesis. Authors: Mehrabian M, Brethour D, Wang H, Xi Z, Rogaeva E, Schmitt-Ulms G PLoS ONE, 2015;10(8):e0133741. Species: Mouse Sample Type: Whole Cells Application: Bioassay Radiation-induced alterations of osteogenic and chondrogenic differentiation of human mesenchymal stem cells. Authors: Cruet-Hennequart S, Drougard C, Shaw G, Legendre F, Demoor M, Barry F, Lefaix J, Galera P PLoS ONE, 2015;10(3):e0119334. Species: Human Sample Type: Whole Cells Application: Bioassay Pyrroloquinoline-quinone suppresses liver fibrogenesis in mice. Authors: Jia D, Duan F, Peng P, Sun L, Ruan Y, Gu J PLoS ONE, 2015;10(3):e0121939. Species: Mouse Sample Type: Whole Cells Application: Bioassay High mobility group protein-mediated transcription requires DNA damage marker gamma-H2AX. Authors: Singh I, Ozturk N, Cordero J, Mehta A, Hasan D, Cosentino C, Sebastian C, Kruger M, Looso M, Carraro G, Bellusci S, Seeger W, Braun T, Mostoslavsky R, Barreto G Cell Res, 2015;25(7):837-50. Species: Mouse Sample Type: Whole Cells Application: Bioassay The PAX3-FOXO1 fusion protein present in rhabdomyosarcoma interferes with normal FOXO activity and the TGF-beta pathway. Authors: Schmitt-Ney M, Camussi G PLoS ONE, 2015;10(3):e0121474. Species: Human Sample Type: Whole Cells Application: Bioassay Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes. Authors: Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, Anselmi F, Rapelli S, Grillo M, Borghi S, Oliviero S Stem Cells, 2015;33(3):742-50. Species: Mouse Sample Type: Whole Cells Application: Bioassay Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Authors: Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, Lesch J, Lu R, Newton K, Huang O, Cochran A, Vasser M, Fauber B, DeVoss J, Webster J, Diehl L, Modrusan Z, Kirkpatrick D, Lill J, Ouyang W, Dixit V Nature, 2015;518(7539):417-21. Species: Mouse Sample Type: Whole Cells Application: Bioassay Human natural killer cell microRNA: differential expression of MIR181A1B1 and MIR181A2B2 genes encoding identical mature microRNAs. Authors: Presnell S, Al-Attar A, Cichocki F, Miller J, Lutz C Genes Immun, 2015;16(1):89-98. Species: Human Sample Type: Whole Cells Application: Bioassay Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. Authors: Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, Guerrouahen B, Rafii S, Rafii A J Transl Med, 2015;13(0):27. Species: Human Sample Type: Whole Cells Application: Bioassay Novel mechanism of macrophage-mediated metastasis revealed in a zebrafish model of tumor development. Authors: Wang J, Cao Z, Zhang X, Nakamura M, Sun M, Hartman J, Harris R, Sun Y, Cao Y Cancer Res, 2015;75(2):306-15. Species: Human Sample Type: Whole Cells Application: Bioassay Cutting edge: epigenetic regulation of Foxp3 defines a stable population of CD4+ regulatory T cells in tumors from mice and humans. Authors: Waight J, Takai S, Marelli B, Qin G, Hance K, Zhang D, Tighe R, Lan Y, Lo K, Sabzevari H, Hofmeister R, Wilson N J Immunol, 2015;194(3):878-82. Species: Mouse Sample Type: Whole Cells Application: Bioassay Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation. Authors: Narcisi R, Cleary M, Brama P, Hoogduijn M, Tuysuz N, ten Berge D, van Osch G Stem Cell Reports, 2015;4(3):459-72. Species: Human Sample Type: Whole Cells Application: Bioassay Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M Invest Ophthalmol Vis Sci, 2015;56(2):1051-62. Species: Human Sample Type: Whole Cells Application: Bioassay Drak2 Does Not Regulate TGF-beta Signaling in T Cells. Authors: Harris T, McGargill M PLoS ONE, 2015;10(5):e0123650. Species: Mouse Sample Type: Whole Cells Application: Bioassay Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. Authors: Giraldo C, Alvarez M, Carmona J BMC Vet Res, 2015;11(0):60. Species: Equine Sample Type: Plasma Lysate 4-1BB ligand signaling to T cells limits T cell activation. Authors: Eun S, Lee S, Xu Y, Croft M J Immunol, 2015;194(1):134-41. Species: Mouse Sample Type: Whole Cells Application: Bioassay 5Z-7-Oxozeanol Inhibits the Effects of TGFbeta1 on Human Gingival Fibroblasts. Authors: Kuk H, Hutchenreuther J, Murphy-Marshman H, Carter D, Leask A PLoS ONE, 2015;10(4):e0123689. Species: Human Sample Type: Whole Cells Application: Bioassay CXCL13 antibody for the treatment of autoimmune disorders. Authors: Klimatcheva E, Pandina T, Reilly C, Torno S, Bussler H, Scrivens M, Jonason A, Mallow C, Doherty M, Paris M, Smith E, Zauderer M BMC Immunol, 2015;16(0):6. Species: Mouse Sample Type: Whole Cells Application: Bioassay Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer. Authors: Togashi, Yosuke, Sakamoto, Hiroki, Hayashi, Hidetosh, Terashima, Masato, de Velasco, Marco A, Fujita, Yoshihik, Kodera, Yasuo, Sakai, Kazuko, Tomida, Shuta, Kitano, Masayuki, Ito, Akihiko, Kudo, Masatosh, Nishio, Kazuto Mol Cancer, 2014;13(0):126. Species: Human Sample Type: Whole Cells Application: Bioassay Induction of galectin-1 by TGF-beta1 accelerates fibrosis through enhancing nuclear retention of Smad2. Authors: Lim M, Ahn J, Yi J, Kim M, Son A, Lee S, Lim D, Kim S, Kang M, Han Y, Song J Exp Cell Res, 2014;326(1):125-35. Species: Human Sample Type: Whole Cells Application: Bioassay The fate of the primary cilium during myofibroblast transition. Authors: Rozycki M, Lodyga M, Lam J, Miranda M, Fatyol K, Speight P, Kapus A Mol Biol Cell, 2014;25(5):643-57. Species: Human Sample Type: Whole Cells Application: Bioassay Src-mediated post-translational regulation of endoglin stability and function is critical for angiogenesis. Authors: Pan C, Kumar S, Shah N, Hoyt D, Hawinkels L, Mythreye K, Lee N J Biol Chem, 2014;289(37):25486-96. Species: Mouse Sample Type: Whole Cells Application: Bioassay Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro. Authors: Italiani, Paola, Mazza, Emilia M, Lucchesi, Davide, Cifola, Ingrid, Gemelli, Claudia, Grande, Alexis, Battaglia, Cristina, Bicciato, Silvio, Boraschi, Diana PLoS ONE, 2014;9(2):e87680. Species: Human Sample Type: Whole Cells Application: Bioassay TGFbeta-mediated suppression of CD248 in non-cancer cells via canonical Smad-dependent signaling pathways is uncoupled in cancer cells. Authors: Suresh Babu S, Valdez Y, Xu A, O'Byrne A, Calvo F, Lei V, Conway E BMC Cancer, 2014;14(0):113. Species: Mouse Sample Type: Whole Cells Application: Bioassay The AMPK agonist AICAR inhibits TGF-beta1 induced activation of kidney myofibroblasts. Authors: Chen K, Hsu H, Lee C, Yen T, Ko Y, Yang C, Hung C PLoS ONE, 2014;9(9):e106554. Species: Rat Sample Type: Whole Cells Application: Bioassay Role of leukocyte cell-derived chemotaxin 2 as a biomarker in hepatocellular carcinoma. Authors: Okabe H, Delgado E, Lee J, Yang J, Kinoshita H, Hayashi H, Tsung A, Behari J, Beppu T, Baba H, Monga S PLoS ONE, 2014;9(6):e98817. Species: Human Sample Type: Whole Cells Application: Bioassay Secreted fibroblast-derived miR-34a induces tubular cell apoptosis in fibrotic kidney. Authors: Zhou Y, Xiong M, Niu J, Sun Q, Su W, Zen K, Dai C, Yang J J Cell Sci, 2014;127(0):4494-506. Species: Rat Sample Type: Whole Cells Application: Bioassay Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis. Authors: Komatsu N, Okamoto K, Sawa S, Nakashima T, Oh-hora M, Kodama T, Tanaka S, Bluestone J, Takayanagi H Nat Med, 2014;20(1):62-8. Species: Mouse Sample Type: Whole Cells Application: Bioassay The E3 deubiquitinase USP17 is a positive regulator of retinoic acid-related orphan nuclear receptor gammat (RORgammat) in Th17 cells. Authors: Han L, Yang J, Wang X, Wu Q, Yin S, Li Z, Zhang J, Xing Y, Chen Z, Tsun A, Li D, Piccioni M, Zhang Y, Guo Q, Jiang L, Bao L, Lv L, Li B J Biol Chem, 2014;289(37):25546-55. Species: Human Sample Type: Whole Cells Application: Bioassay Phospholipase D1 mediates lymphocyte adhesion and migration in experimental autoimmune encephalomyelitis. Authors: Gobel K, Schuhmann M, Pankratz S, Stegner D, Herrmann A, Braun A, Breuer J, Bittner S, Ruck T, Wiendl H, Kleinschnitz C, Nieswandt B, Meuth S Eur J Immunol, 2014;44(8):2295-305. Species: Mouse Sample Type: Whole Cells Application: Bioassay Influenza promotes collagen deposition via alphavbeta6 integrin-mediated transforming growth factor beta activation. Authors: Jolly L, Stavrou A, Vanderstoken G, Meliopoulos V, Habgood A, Tatler A, Porte J, Knox A, Weinreb P, Violette S, Hussell T, Kolb M, Stampfli M, Schultz-Cherry S, Jenkins G J Biol Chem, 2014;289(51):35246-63. Species: Human Sample Type: Whole Cells Application: Bioassay Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. Authors: Olderoy M, Lilledahl M, Beckwith M, Melvik J, Reinholt F, Sikorski P, Brinchmann J PLoS ONE, 2014;9(3):e91662. Species: Human Sample Type: Whole Cells Application: Bioassay Analysis of the effects of five factors relevant to in vitro chondrogenesis of human mesenchymal stem cells using factorial design and high throughput mRNA-profiling. Authors: Jakobsen R, Ostrup E, Zhang X, Mikkelsen T, Brinchmann J PLoS ONE, 2014;9(5):e96615. Species: Human Sample Type: Whole Cells Application: Bioassay Role of Blimp-1 in programing Th effector cells into IL-10 producers. Authors: Neumann C, Heinrich F, Neumann K, Junghans V, Mashreghi M, Ahlers J, Janke M, Rudolph C, Mockel-Tenbrinck N, Kuhl A, Heimesaat M, Esser C, Im S, Radbruch A, Rutz S, Scheffold A J Exp Med, 2014;211(9):1807-19. Species: Mouse Sample Type: Whole Cells Application: Bioassay Characteristics of TCR/CD3 complex CD3{varepsilon} chains of regulatory CD4+ T (Treg) lymphocytes: role in Treg differentiation in vitro and impact on Treg in vivo. Authors: Rojo J, Ojeda G, Acosta Y, Montes-Casado M, Criado G, Portoles P J Leukoc Biol, 2014;95(3):441-50. Species: Mouse Sample Type: Whole Cells Application: Bioassay Glucocorticoids recruit Tgfbr3 and Smad1 to shift transforming growth factor-beta signaling from the Tgfbr1/Smad2/3 axis to the Acvrl1/Smad1 axis in lung fibroblasts. Authors: Schwartze J, Becker S, Sakkas E, Wujak L, Niess G, Usemann J, Reichenberger F, Herold S, Vadasz I, Mayer K, Seeger W, Morty R J Biol Chem, 2014;289(6):3262-75. Species: Human Sample Type: Whole Cells Application: Bioassay Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing. Authors: Bohnsack R, Warejcka D, Wang L, Gillespie S, Bernstein A, Twining S, Dahms N Invest Ophthalmol Vis Sci, 2014;55(12):7697-708. Species: Human Sample Type: Whole Cells Application: Bioassay Trim28 contributes to EMT via regulation of E-cadherin and N-cadherin in lung cancer cell lines. Authors: Chen L, Munoz-Antonia T, Cress W PLoS ONE, 2014;9(7):e101040. Species: Human Sample Type: Whole Cells Application: Bioassay Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling. Authors: Santhekadur P, Akiel M, Emdad L, Gredler R, Srivastava J, Rajasekaran D, Robertson C, Mukhopadhyay N, Fisher P, Sarkar D FEBS Open Bio, 2014;4(0):353-61. Species: Human Sample Type: Whole Cells Application: Bioassay Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression. Authors: Chen C, Akiyama K, Yamaza T, You Y, Xu X, Li B, Zhao Y, Shi S EMBO Mol Med, 2014;6(3):322-34. Species: Human Sample Type: Whole Cells Application: Bioassay Differentiation of IL-17-producing effector and regulatory human T cells from lineage-committed naive precursors. Authors: Mercer F, Khaitan A, Kozhaya L, Aberg J, Unutmaz D J Immunol, 2014;193(3):1047-54. Species: Human Sample Type: Whole Cells Application: Bioassay TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin. Authors: Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R PLoS ONE, 2014;9(4):e94801. Species: Human Sample Type: Whole Cells Application: Bioassay Follicular dendritic cell secreted protein FDC-SP controls IgA production. Authors: Hou S, Landego I, Jayachandran N, Miller A, Gibson I, Ambrose C, Marshall A Mucosal Immunol, 2014;7(4):948-57. Species: Mouse Sample Type: Whole Cells Application: Bioassay B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets. Authors: Kuang D, Xiao X, Zhao Q, Chen M, Li X, Liu R, Wei Y, Ouyang F, Chen D, Wu Y, Lao X, Deng H, Zheng L J Clin Invest, 2014;124(10):4657-67. Species: Human Sample Type: Whole Cells Application: Bioassay The broad-spectrum metalloproteinase inhibitor BB-94 inhibits growth, HER3 and Erk activation in fulvestrant-resistant breast cancer cell lines. Authors: Kirkegaard T, Yde C, Kveiborg M, Lykkesfeldt A Int J Oncol, 2014;45(1):393-400. Species: Human Sample Type: Whole Cells Application: Bioassay Human chondrocytes respond discordantly to the protein encoded by the osteoarthritis susceptibility gene GDF5. Authors: Ratnayake M, Ploger F, Santibanez-Koref M, Loughlin J PLoS ONE, 2014;9(1):e86590. Species: Human Sample Type: Whole Cells Application: Bioassay Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy. Authors: Xiao, Guan-Yu, Liu, I-Hsuan, Cheng, Chun-Chu, Chang, Chia-Chu, Lee, Yen-Hua, Cheng, Winston, Wu, Shinn-Ch PLoS ONE, 2014;9(9):e106538. Species: Mouse Sample Type: Whole Cells Application: Bioassay Gene expression profiles of human adipose tissue-derived mesenchymal stem cells are modified by cell culture density. Authors: Kim D, Lee M, Yoo K, Lee T, Kim H, Jang I, Chun Y, Kim H, Park S, Lee S, Son M, Jung H, Sung K, Koo H PLoS ONE, 2014;9(1):e83363. Species: Human Sample Type: Whole Cells Application: cell culture Protein tyrosine phosphatase alpha mediates profibrotic signaling in lung fibroblasts through TGF-beta responsiveness. Authors: Aschner Y, Khalifah A, Briones N, Yamashita C, Dolgonos L, Young S, Campbell M, Riches D, Redente E, Janssen W, Henson P, Sap J, Vacaresse N, Kapus A, McCulloch C, Zemans R, Downey G Am J Pathol, 2014;184(5):1489-502. Species: Mouse Sample Type: Whole Cells Application: Bioassay COL11A1 promotes tumor progression and predicts poor clinical outcome in ovarian cancer. Authors: Wu Y, Chang T, Huang Y, Huang H, Chou C Oncogene, 2014;33(26):3432-40. Species: Human Sample Type: Whole Cells Application: Bioassay Chromatinized protein kinase C-theta directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells. Authors: Zafar A, Wu F, Hardy K, Li J, Tu W, McCuaig R, Harris J, Khanna K, Attema J, Gregory P, Goodall G, Harrington K, Dahlstrom J, Boulding T, Madden R, Tan A, Milburn P, Rao S Mol Cell Biol, 2014;34(16):2961-80. Species: Human Sample Type: Whole Cells Application: Bioassay Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis. Authors: Karki S, Surolia R, Hock T, Guroji P, Zolak J, Duggal R, Ye T, Thannickal V, Antony V FASEB J, 2014;28(3):1122-31. Species: Human Sample Type: Whole Cells Application: Bioassay Dual role of acetaminophen in promoting hepatoma cell apoptosis and kidney fibroblast proliferation. Authors: Yu Y, Yiang G, Chou P, Tseng H, Wu T, Hung Y, Lin P, Lin S, Liu H, Chang W, Wei C Mol Med Rep, 2014;9(6):2077-84. Species: Rat Sample Type: Whole Cells Application: Bioassay A signal transduction pathway from TGF-beta1 to SKP2 via Akt1 and c-Myc and its correlation with progression in human melanoma. Authors: Qu X, Shen L, Zheng Y, Cui Y, Feng Z, Liu F, Liu J J Invest Dermatol, 2014;134(1):159-67. Species: Human Sample Type: Whole Cells Application: Bioassay Caffeine and rolipram affect Smad signalling and TGF-beta1 stimulated CTGF and transgelin expression in lung epithelial cells. Authors: Fehrholz M, Speer C, Kunzmann S PLoS ONE, 2014;9(5):e97357. Species: Human Sample Type: Whole Cells Application: Bioassay TGF-beta/Smad3 stimulates stem cell/developmental gene expression and vascular smooth muscle cell de-differentiation. Authors: Shi X, DiRenzo D, Guo L, Franco S, Wang B, Seedial S, Kent K , 2014;9(0):. Species: Rat Sample Type: Whole Cells Application: Bioassay Transcriptional regulation of seprase in invasive melanoma cells by transforming growth factor-beta signaling. Authors: Tulley S, Chen W J Biol Chem, 2014;289(22):15280-96. Species: Human Sample Type: Whole Cells Application: Bioassay Inhibitory effects of PPARgamma ligands on TGF-beta1-induced corneal myofibroblast transformation. Authors: Jeon K, Kulkarni A, Woeller C, Phipps R, Sime P, Hindman H, Huxlin K Am J Pathol, 2014;184(5):1429-45. Species: Feline Sample Type: Whole Cells Application: Bioassay MicroRNA-7 inhibition rescues age-associated loss of epidermal growth factor receptor and hyaluronan-dependent differentiation in fibroblasts. Authors: Midgley A, Bowen T, Phillips A, Steadman R Aging Cell, 2014;13(2):235-44. Species: Human Sample Type: Whole Cells Application: Bioassay Independent adipogenic and contractile properties of fibroblasts in Graves' orbitopathy: an in vitro model for the evaluation of treatments. Authors: Li H, Fitchett C, Kozdon K, Jayaram H, Rose G, Bailly M, Ezra D PLoS ONE, 2014;9(4):e95586. Species: Human Sample Type: Whole Cells Application: Bioassay Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosing. Authors: Wei D, Chiang V, Fyne E, Balakrishnan M, Barnes T, Graupe M, Hesselgesser J, Irrinki A, Murry J, Stepan G, Stray K, Tsai A, Yu H, Spindler J, Kearney M, Spina C, McMahon D, Lalezari J, Sloan D, Mellors J, Geleziunas R, Cihlar T PLoS Pathog, 2014;10(4):e1004071. Species: Human Sample Type: Whole Cells Application: Bioassay Musashi-2 controls cell fate, lineage bias, and TGF-beta signaling in HSCs. Authors: Park S, Deering R, Lu Y, Tivnan P, Lianoglou S, Al-Shahrour F, Ebert B, Hacohen N, Leslie C, Daley G, Lengner C, Kharas M J Exp Med, 2014;211(1):71-87. Species: Human Sample Type: Whole Cells Application: Bioassay Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils. Authors: Baldwin A, Cain S, Lennon R, Godwin A, Merry C, Kielty C J Cell Sci, 2014;127(0):158-71. Species: Human Sample Type: Whole Cells Application: Bioassay Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis. Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H J Clin Invest, 2014;124(1):448-60. Species: Human Sample Type: Whole Cells Application: Bioassay JAK/STAT3 signaling is required for TGF-beta-induced epithelial-mesenchymal transition in lung cancer cells. Authors: Liu R, Zeng Y, Lei Z, Wang L, Yang H, Liu Z, Zhao J, Zhang H Int J Oncol, 2014;44(5):1643-51. Species: Human Sample Type: Whole Cells Application: Bioassay Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells. Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C PLoS ONE, 2014;9(5):e96695. Species: Human Sample Type: Whole Cells Application: Bioassay CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism. Authors: Miller M, Petty C, Tompkins M, Fogle J Virol J, 2014;11(0):7. Species: Feline Sample Type: Whole Cells Application: Bioassay Cystatin B is a progression marker of human epithelial ovarian tumors mediated by the TGF-beta signaling pathway. Authors: Wang X, Gui L, Zhang Y, Zhang J, Shi J, Xu G Int J Oncol, 2014;44(4):1099-106. Species: Human Sample Type: Whole Cells Application: Bioassay Transforming growth factor-beta-induced miR143 expression in regulation of non-small cell lung cancer cell viability and invasion capacity in vitro and in vivo. Authors: Cheng T, Hu C, Yang H, Cao L, An J Int J Oncol, 2014;45(5):1977-88. Species: Human Sample Type: Whole Cells Application: Bioassay Transforming growth factor beta signaling overcomes dasatinib resistance in lung cancer. Authors: Gordian E, Li J, Pevzner Y, Mediavilla-Varela M, Luddy K, Ohaegbulam K, Daniel K, Haura E, Munoz-Antonia T PLoS ONE, 2014;9(12):e114131. Species: Human Sample Type: Whole Cells Application: Bioassay Effective chemoimmunotherapy with anti-TGFbeta antibody and cyclophosphamide in a mouse model of breast cancer. Authors: Chen X, Yang Y, Zhou Q, Weiss J, Howard O, McPherson J, Wakefield L, Oppenheim J PLoS ONE, 2014;9(1):e85398. Species: Mouse Sample Type: Whole Cells Application: Bioassay The roles of the epithelial-mesenchymal transition marker PRRX1 and miR-146b-5p in papillary thyroid carcinoma progression. Authors: Hardin H, Guo Z, Shan W, Montemayor-Garcia C, Asioli S, Yu X, Harrison A, Chen H, Lloyd R Am J Pathol, 2014;184(8):2342-54. Species: Human Sample Type: Whole Cells Application: Bioassay Curcumin up-regulates phosphatase and tensin homologue deleted on chromosome 10 through microRNA-mediated control of DNA methylation--a novel mechanism suppressing liver fibrosis. Authors: Zheng J, Wu C, Lin Z, Guo Y, Shi L, Dong P, Lu Z, Gao S, Liao Y, Chen B, Yu F FEBS J, 2014;281(1):88-103. Species: Rat Sample Type: Whole Cells Application: Bioassay Coronary heart disease-associated variation in TCF21 disrupts a miR-224 binding site and miRNA-mediated regulation. Authors: Miller C, Haas U, Diaz R, Leeper N, Kundu R, Patlolla B, Assimes T, Kaiser F, Perisic L, Hedin U, Maegdefessel L, Schunkert H, Erdmann J, Quertermous T, Sczakiel G PLoS Genet, 2014;10(3):e1004263. Species: Human Sample Type: Whole Cells Application: Bioassay Negative regulation of NADPH oxidase 4 by hydrogen peroxide-inducible clone 5 (Hic-5) protein. Authors: Desai L, Zhou Y, Estrada A, Ding Q, Cheng G, Collawn J, Thannickal V J Biol Chem, 2014;289(26):18270-8. Species: Human Sample Type: Whole Cells Application: Bioassay Loss of Dab2 expression in breast cancer cells impairs their ability to deplete TGF-beta and induce Tregs development via TGF-beta. Authors: Xu S, Zhu J, Wu Z PLoS ONE, 2014;9(3):e91709. Species: Human Sample Type: Whole Cells Application: Bioassay Detection of atomic scale changes in the free volume void size of three-dimensional colorectal cancer cell culture using positron annihilation lifetime spectroscopy. Authors: Axpe E, Lopez-Euba T, Castellanos-Rubio A, Merida D, Garcia J, Plaza-Izurieta L, Fernandez-Jimenez N, Plazaola F, Bilbao J PLoS ONE, 2014;9(1):e83838. Species: Human Sample Type: Whole Cells Application: Bioassay Induction of immunoregulatory CD271+ cells by metastatic tumor cells that express human endogenous retrovirus H. Authors: Kudo-Saito C, Yura M, Yamamoto R, Kawakami Y Cancer Res, 2014;74(5):1361-70. Species: Human Sample Type: Whole Cells Application: Bioassay Combined targeting of TGF-beta1 and integrin beta3 impairs lymph node metastasis in a mouse model of non-small-cell lung cancer. Authors: Salvo E, Garasa S, Dotor J, Morales X, Pelaez R, Altevogt P, Rouzaut A Mol Cancer, 2014;13(0):112. Species: Human Sample Type: Whole Cells Application: Bioassay Midkine-deficiency delays chondrogenesis during the early phase of fracture healing in mice. Authors: Haffner-Luntzer, Melanie, Heilmann, Aline, Rapp, Anna Eli, Beie, Simon, Schinke, Thorsten, Amling, Michael, Ignatius, Anita, Liedert, Astrid PLoS ONE, 2014;9(12):e116282. Species: Mouse Sample Type: Whole Cells Application: Bioassay Inhibition of periostin expression protects against the development of renal inflammation and fibrosis. Authors: Mael-Ainin M, Abed A, Conway S, Dussaule J, Chatziantoniou C J Am Soc Nephrol, 2014;25(8):1724-36. Species: Human Sample Type: Whole Cells Application: Bioassay Epithelial splicing regulatory protein 1 is a favorable prognostic factor in pancreatic cancer that attenuates pancreatic metastases. Authors: Ueda J, Matsuda Y, Yamahatsu K, Uchida E, Naito Z, Korc M, Ishiwata T Oncogene, 2014;33(36):4485-95. Species: Human Sample Type: Whole Cells Application: Bioassay The anti-proliferative function of the TGF-beta1 signaling pathway involves the repression of the oncogenic TBX2 by its homologue TBX3. Authors: Li J, Ballim D, Rodriguez M, Cui R, Goding C, Teng H, Prince S J Biol Chem, 2014;289(51):35633-43. Species: Human Sample Type: Whole Cells Application: Bioassay Binding of AID to DNA does not correlate with mutator activity. Authors: Matthews A, Husain S, Chaudhuri J J Immunol, 2014;193(1):252-7. Species: Mouse Sample Type: Whole Cells Application: Bioassay VDR activity is differentially affected by Hic-5 in prostate cancer and stromal cells. Authors: Solomon J, Heitzer M, Liu T, Beumer J, Parise R, Normolle D, Leach D, Buchanan G, DeFranco D Mol Cancer Res, 2014;12(8):1166-80. Species: Human Sample Type: Whole Cells Application: Bioassay JNK inhibitor SP600125 enhances TGF-beta-induced apoptosis of RBE human cholangiocarcinoma cells in a Smad-dependent manner. Authors: Lin Y, Zhang B, Liang H, Lu Y, Ai X, Zhang B, Chen X Mol Med Rep, 2013;8(6):1623-9. Species: Human Sample Type: Whole Cells Application: Bioassay Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium. Authors: Hojo, Hironori, Ohba, Shinsuke, Taniguchi, Kiyomi, Shirai, Masataka, Yano, Fumiko, Saito, Taku, Ikeda, Toshiyuk, Nakajima, Keiji, Komiyama, Yuske, Nakagata, Naomi, Suzuki, Kentaro, Mishina, Yuji, Yamada, Masahisa, Konno, Tomohiro, Takato, Tsuyoshi, Kawaguchi, Hiroshi, Kambara, Hideki, Chung, Ung-il J Biol Chem, 2013;288(14):9924-32. Species: Mouse Sample Type: Whole Tissue Application: Bioassay Neuropilin-2 Is upregulated in lung cancer cells during TGF-beta1-induced epithelial-mesenchymal transition. Authors: Nasarre P, Gemmill R, Potiron V, Roche J, Lu X, Baron A, Korch C, Garrett-Mayer E, Lagana A, Howe P, Drabkin H Cancer Res, 2013;73(23):7111-21. Species: Human Sample Type: Whole Cells Application: Bioassay TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells. Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F Cancer Res, 2013;73(7):2333-44. Species: Human Sample Type: Whole Cells Application: Bioassay FOG2 protein down-regulation by transforming growth factor-beta1-induced microRNA-200b/c leads to Akt kinase activation and glomerular mesangial hypertrophy related to diabetic nephropathy. Authors: Park J, Kato M, Yuan H, Castro N, Lanting L, Wang M, Natarajan R J Biol Chem, 2013;288(31):22469-80. Species: Mouse Sample Type: Whole Cells Application: Bioassay Transforming growth factor ÃŽÂ²-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis. Am J Pathol, 2013;182(1):118-31. Species: Mouse Sample Type: Whole Cells Application: Bioassay MicroRNA-22 is a master regulator of bone morphogenetic protein-7/6 homeostasis in the kidney. Authors: Long, Jianyin, Badal, Shawn S, Wang, Yin, Chang, Benny H, Rodriguez, Antony, Danesh, Farhad R J Biol Chem, 2013;288(51):36202-14. Species: Mouse Sample Type: Whole Cells Application: Bioassay MiR-1 and miR-200 inhibit EMT via Slug-dependent and tumorigenesis via Slug-independent mechanisms. Authors: Liu Y, Yin J, Abou-Kheir W, Hynes P, Casey O, Fang L, Yi M, Stephens R, Seng V, Sheppard-Tillman H, Martin P, Kelly K Oncogene, 2013;32(3):296-306. Species: Mouse Sample Type: Whole Cells Application: Bioassay Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement. Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C Arthritis Rheum, 2013;65(5):1347-56. Species: Human Sample Type: Whole Cells Application: Bioassay Canonical transforming growth factor-beta signaling regulates disintegrin metalloprotease expression in experimental renal fibrosis via miR-29. Authors: Ramdas V, McBride M, Denby L, Baker A Am J Pathol, 2013;183(6):1885-96. Species: Rat Sample Type: Whole Cells Application: Bioassay Butyrophilin Btn2a2 inhibits TCR activation and phosphatidylinositol 3-kinase/Akt pathway signaling and induces Foxp3 expression in T lymphocytes. Authors: Ammann J, Cooke A, Trowsdale J J Immunol, 2013;190(10):5030-6. Species: Mouse Sample Type: Whole Cells Application: Bioassay Role of IGF-1 pathway in lung fibroblast activation. Authors: Hung C, Rohani M, Lee S, Chen P, Schnapp L Respir Res, 2013;14(0):102. Species: Mouse Sample Type: Whole Cells Application: cell culture Src-mediated morphology transition of lung cancer cells in three-dimensional organotypic culture. Authors: Nguyen H, Zhuang Y, Sun L, Kantrow S, Kolls J, You Z, Zhuo Y, Shan B Cancer Cell Int, 2013;13(1):16. Species: Human Sample Type: Whole Cells Application: Bioassay TGF-Beta suppresses VEGFA-mediated angiogenesis in colon cancer metastasis. Authors: Geng L, Chaudhuri A, Talmon G, Wisecarver J, Wang J PLoS ONE, 2013;8(3):e59918. Species: Human Sample Type: Whole Cells Application: Bioassay Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells. Authors: Wang C, Yi T, Qin L, Maldonado R, von Andrian U, Kulkarni S, Tellides G, Pober J J Clin Invest, 2013;123(4):1677-93. Species: Human Sample Type: Whole Cells Application: Bioassay Kynurenine 3-monooxygenase mediates inhibition of Th17 differentiation via catabolism of endogenous aryl hydrocarbon receptor ligands. Authors: Stephens G, Wang Q, Swerdlow B, Bhat G, Kolbeck R, Fung M Eur J Immunol, 2013;43(7):1727-34. Species: Mouse Sample Type: Whole Cells Application: Bioassay TGF-beta1 regulation of estrogen production in mature rat Leydig cells. Authors: Liu M, Wang H, Wang Z, Zhang Y, Chen Y, Zhu F, Zhang Y, Ma J, Li Z PLoS ONE, 2013;8(3):e60197. Species: Rat Sample Type: Whole Cells Application: Bioassay Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells. Authors: Mae, Shin-Ich, Shono, Akemi, Shiota, Fumihiko, Yasuno, Tetsuhik, Kajiwara, Masatosh, Gotoda-Nishimura, Nanaka, Arai, Sayaka, Sato-Otubo, Aiko, Toyoda, Taro, Takahashi, Kazutosh, Nakayama, Naoki, Cowan, Chad A, Aoi, Takashi, Ogawa, Seishi, McMahon, Andrew P, Yamanaka, Shinya, Osafune, Kenji Nat Commun, 2013;4(0):1367. Species: Human Sample Type: Whole Cells Application: Bioassay Loss of canonical Smad4 signaling promotes KRAS driven malignant transformation of human pancreatic duct epithelial cells and metastasis. Authors: Leung L, Radulovich N, Zhu C, Wang D, To C, Ibrahimov E, Tsao M PLoS ONE, 2013;8(12):e84366. Species: Human Sample Type: Whole Cells Application: Bioassay Foxm1 transcription factor is required for lung fibrosis and epithelial-to-mesenchymal transition. Authors: Balli D, Ustiyan V, Zhang Y, Wang I, Masino A, Ren X, Whitsett J, Kalinichenko V, Kalin T EMBO J, 2013;32(2):231-44. Species: Human Sample Type: Whole Cells Application: Bioassay Identification of bone morphogenetic protein 7 (BMP7) as an instructive factor for human epidermal Langerhans cell differentiation. Authors: Yasmin N, Bauer T, Modak M, Wagner K, Schuster C, Koffel R, Seyerl M, Stockl J, Elbe-Burger A, Graf D, Strobl H J Exp Med, 2013;210(12):2597-610. Species: Human Sample Type: Whole Cells Application: Bioassay TNF-like ligand 1A (TL1A) gene knockout leads to ameliorated collagen-induced arthritis in mice: implication of TL1A in humoral immune responses. Authors: Wang X, Hu Y, Charpentier T, Lamarre A, Qi S, Wu J, Luo H J Immunol, 2013;191(11):5420-9. Species: Mouse Sample Type: Whole Cells Application: Bioassay Cell adhesion and shape regulate TGF-beta1-induced epithelial-myofibroblast transition via MRTF-A signaling. Authors: O'Connor J, Gomez E PLoS ONE, 2013;8(12):e83188. Species: Mouse Sample Type: Whole Cells Application: Bioassay Human hair follicle stem cell differentiation into contractile smooth muscle cells is induced by transforming growth factor-beta1 and platelet-derived growth factor BB. Authors: Xu Z, Zhang Q, Li H Mol Med Rep, 2013;8(6):1715-21. Species: Human Sample Type: Whole Cells Application: Bioassay Blockade of Smad signaling by 3'-deoxyadenosine: a mechanism for its anti-fibrotic potential. Authors: Gu L, Johno H, Nakajima S, Kato H, Takahashi S, Katoh R, Kitamura M Lab Invest, 2013;93(4):450-61. Species: Rat Sample Type: Whole Cells Application: Bioassay The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of human tegumentary leishmaniasis. Authors: Oliveira W, Ribeiro L, Schrieffer A, Machado P, Carvalho E, Bacellar O Cytokine, 2013;66(2):127-32. Species: Human Sample Type: Whole Cells Application: Bioassay Dendritic cell podosome dynamics does not depend on the F-actin regulator SWAP-70. Authors: Gotz A, Jessberger R PLoS ONE, 2013;8(3):e60642. Species: Mouse Sample Type: Tissue Homogenates Application: Bioassay Low PIP4K2B expression in human breast tumors correlates with reduced patient survival: A role for PIP4K2B in the regulation of E-cadherin expression. Authors: Keune W, Sims A, Jones D, Bultsma Y, Lynch J, Jirstrom K, Landberg G, Divecha N Cancer Res, 2013;73(23):6913-25. Species: Human Sample Type: Whole Cells Application: Bioassay TGF-beta1 promotes lymphangiogenesis during peritoneal fibrosis. Authors: Kinashi H, Ito Y, Mizuno M, Suzuki Y, Terabayashi T, Nagura F, Hattori R, Matsukawa Y, Mizuno T, Noda Y, Nishimura H, Nishio R, Maruyama S, Imai E, Matsuo S, Takei Y J Am Soc Nephrol, 2013;24(10):1627-42. Species: Human Sample Type: Whole Cells Application: Bioassay Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin. Authors: Tannetta D, Dragovic R, Gardiner C, Redman C, Sargent I PLoS ONE, 2013;8(2):e56754. Species: Human Sample Type: Whole Cells Application: Bioassay H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs. Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing Nat Genet, 2013;45(1):34-42. Species: Mouse Sample Type: Whole Cells Application: Bioassay Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination. Authors: Masani, Shahnaz, Han, Li, Yu, Kefei Mol Cell Biol, 2013;33(7):1468-73. Species: Mouse Sample Type: Whole Cells Application: Bioassay Downregulation of Dickkopf-3 disrupts prostate acinar morphogenesis through TGF-beta/Smad signalling. Authors: Romero D, Kawano Y, Bengoa N, Walker M, Maltry N, Niehrs C, Waxman J, Kypta R J Cell Sci, 2013;126(0):1858-67. Species: Human Sample Type: Whole Cells Application: Bioassay Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma. Authors: Huse K, Bakkebo M, Walchli S, Oksvold M, Hilden V, Forfang L, Bredahl M, Liestol K, Alizadeh A, Smeland E, Myklebust J PLoS ONE, 2012;7(10):e46117. Species: Human Sample Type: Whole Cells Application: Bioassay Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Nature, 2012;487(7408):505-9. Species: Human Sample Type: Whole Cells Application: Bioassay Tumor Microenvironment Macrophage Inhibitory Factor Directs the Accumulation of Interleukin-17-producing Tumor-infiltrating Lymphocytes and Predicts Favorable Survival in Nasopharyngeal Carcinoma Patients. Authors: Li J, Mo H, Xiong G, Zhang L, He J, Huang Z, Liu Z, Chen Q, Du Z, Zheng L, Qian C, Zeng Y J Biol Chem, 2012;287(42):35484-95. Species: Human Sample Type: Whole Cells Application: Bioassay Hic-5 promotes invadopodia formation and invasion during TGF-beta-induced epithelial-mesenchymal transition. Authors: Pignatelli J, Tumbarello DA, Schmidt RP, Turner CE J. Cell Biol., 2012;197(3):421-37. Species: Human Sample Type: Whole Cells Application: Bioassay Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway. Authors: Bruce D, Macartney T, Yong W, Shou W, Sapkota G Cell Signal, 2012;24(11):1999-2006. Species: Human Sample Type: Whole Cells Application: Bioassay Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. Species: Human Sample Type: Whole Cells Application: Bioassay Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis. Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M Lab Invest, 2012;92(12):1712-25. Species: Human Sample Type: Whole Cells Application: Bioassay A Local Paracrine and Endocrine Network Involving TGF beta, Cox-2, ROS, and Estrogen Receptor beta Influences Reactive Stromal Cell Regulation of Prostate Cancer Cell Motility. Authors: Grubisha MJ, Cifuentes ME, Hammes SR, Defranco DB Mol. Endocrinol., 2012;26(6):940-54. Species: Human Sample Type: Whole Cells Application: Bioassay Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S PLoS Pathog., 2012;8(1):e1002486. Species: Human Sample Type: Whole Cells Application: Bioassay Matrix metalloproteinase-13 (MMP-13) directly and indirectly promotes tumor angiogenesis. Authors: Kudo, Yasusei, Iizuka, Shinji, Yoshida, Maki, Tsunematsu, Takaaki, Kondo, Tomoyuki, Subarnbhesaj, Ajiravud, Deraz, Elsayed, Siriwardena, Samadara, Tahara, Hidetosh, Ishimaru, Naozumi, Ogawa, Ikuko, Takata, Takashi J Biol Chem, 2012;287(46):38716-28. Species: Human Sample Type: Whole Cells Application: Bioassay Non-Canonical Smads Phosphorylation Induced by the Glutamate Release Inhibitor, Riluzole, through GSK3 Activation in Melanoma. Authors: Abushahba W, Olabisi O, Jeong B, Boregowda R, Wen Y, Liu F, Goydos J, Lasfar A, Cohen-Solal K PLoS ONE, 2012;7(10):e47312. Species: Human Sample Type: Whole Cells Application: Bioassay TGF beta1-Induced Baf60c Regulates both Smooth Muscle Cell Commitment and Quiescence. Authors: Sohni A, Mulas F, Ferrazzi F, Luttun A, Bellazzi R, Huylebroeck D, Ekker S, Verfaillie C PLoS ONE, 2012;7(10):e47629. Species: Rat Sample Type: Whole Cells Application: Bioassay Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15. Authors: Mottershead D, Ritter L, Gilchrist R Mol Hum Reprod, 2012;18(3):121-8. Species: Mouse Sample Type: Whole Cells Application: Bioassay Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS. Authors: Flanagan K, Fitzgerald K, Baker J PLoS ONE, 2012;7(7):e40443. Species: Human Sample Type: Whole Cells Application: Bioassay T-Lymphocytes Enable Osteoblast Maturation via IL-17F during the Early Phase of Fracture Repair. Authors: Nam D, Mau E, Wang Y, Wright D, Silkstone D, Whetstone H, Whyne C, Alman B PLoS ONE, 2012;7(6):e40044. Species: Mouse Sample Type: Whole Cells Application: Bioassay IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P PLoS Biol, 2012;10(9):e1001395. Species: Human Sample Type: Whole Cells Application: Bioassay Progenitor-like traits contribute to patient survival and prognosis in oligodendroglial tumors. Clin. Cancer Res., 2012;18(15):4122-35. Species: Human Sample Type: Whole Cells Application: Bioassay CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential. Authors: Radulovic K, Manta C, Rossini V, Holzmann K, Kestler HA, Wegenka UM, Nakayama T, Niess JH J. Immunol., 2012;188(4):2001-13. Species: Mouse Sample Type: Whole Cells Application: Bioassay Targeting Axl with an high-affinity inhibitory aptamer. Authors: Cerchia, Laura, Esposito, Carla L, Camorani, Simona, Rienzo, Anna, Stasio, Loredana, Insabato, Luigi, Affuso, Andrea, de Franciscis, Vittorio Mol Ther, 2012;20(12):2291-303. Species: Human Sample Type: Whole Cells Application: Bioassay Host-derived MCP-1 and MIP-1alpha regulate protective anti-tumor immunity to localized and metastatic B16 melanoma. Authors: Nakasone Y, Fujimoto M, Matsushita T, Hamaguchi Y, Huu DL, Yanaba M, Sato S, Takehara K, Hasegawa M Am. J. Pathol., 2012;180(1):365-74. Species: Mouse Sample Type: In Vivo Application: In Vivo Robust tumor immunity to melanoma mediated by interleukin-9-producing T cells. Nat. Med., 2012;1248(0):18. Species: Mouse Sample Type: Whole Cells Application: Bioassay Antigen-independent differentiation and maintenance of effector-like resident memory T cells in tissues. Authors: Casey KA, Fraser KA, Schenkel JM, Moran A, Abt MC, Beura LK, Lucas PJ, Artis D, Wherry EJ, Hogquist K, Vezys V, Masopust D J. Immunol., 2012;188(10):4866-75. Species: Mouse Sample Type: Whole Cells Application: Bioassay Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA FASEB J., 2011;25(1):409-19. Species: Mouse Sample Type: Whole Cells Application: Bioassay The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function. Authors: Jin W, Qi S, Luo H BMC Immunol., 2011;12(0):68. Species: Mouse Sample Type: Whole Cells Application: Bioassay Aryl hydrocarbon receptor deficiency in T cells suppresses the development of collagen-induced arthritis. Authors: Nakahama T, Kimura A, Nguyen NT, Chinen I, Hanieh H, Nohara K, Fujii-Kuriyama Y, Kishimoto T Proc. Natl. Acad. Sci. U.S.A., 2011;108(34):14222-7. Species: Mouse Sample Type: Whole Cells Application: Bioassay Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha. Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM J. Biol. Chem., 2011;286(2):961-75. Species: Human Sample Type: Whole Cells Application: Bioassay Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4. Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A J. Biol. Chem., 2011;286(32):28097-110. Species: Human Sample Type: Whole Cells Application: Bioassay Colorectal cancer cells activate adjacent fibroblasts resulting in FGF1/FGFR3 signaling and increased invasion. Authors: Henriksson ML, Edin S, Dahlin AM, Oldenborg PA, Oberg Ã…, Van Guelpen B, Rutegard J, Stenling R, Palmqvist R Am. J. Pathol., 2011;178(3):1387-94. Species: Human Sample Type: Whole Cells Application: Bioassay Transcription factor Dlx2 protects from TGFbeta-induced cell-cycle arrest and apoptosis. Authors: Yilmaz M, Maass D, Tiwari N, Waldmeier L, Schmidt P, Lehembre F, Christofori G EMBO J., 2011;30(21):4489-99. Species: Mouse Sample Type: Whole Cells Application: Bioassay C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY Diabetologia, 2011;54(10):2713-23. Species: N/A Sample Type: N/A Application: ELISA Standard Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner. Authors: Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S Immunology, 2011;133(3):278-87. Species: Human Sample Type: Whole Cells Application: Bioassay Development and characterisation of an assay for furin activity. Authors: Bourne GL, Grainger DJ J. Immunol. Methods, 2011;364(1):101-8. Species: Human Sample Type: Whole Cells Application: Bioassay The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF. Authors: El-Behi M, Ciric B, Dai H Nat. Immunol., 2011;12(6):568-75. Species: Mouse Sample Type: Whole Cells Application: Bioassay Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction. Authors: Kato N, Kosugi T, Sato W, Ishimoto T, Kojima H, Sato Y, Sakamoto K, Maruyama S, Yuzawa Y, Matsuo S, Kadomatsu K Am. J. Pathol., 2011;178(2):572-9. Species: Mouse Sample Type: Whole Cells Application: Bioassay Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens. Authors: DePaolo RW, Abadie V, Tang F Nature, 2011;471(7337):220-4. Species: Mouse Sample Type: Whole Cells Application: Bioassay Human tumors instigate granulin-expressing hematopoietic cells that promote malignancy by activating stromal fibroblasts in mice. Authors: Elkabets M, Gifford AM, Scheel C, Nilsson B, Reinhardt F, Bray MA, Carpenter AE, Jirstrom K, Magnusson K, Ebert BL, Ponten F, Weinberg RA, McAllister SS J. Clin. Invest., 2011;121(2):784-99. Species: Human Sample Type: Whole Cells Application: Bioassay HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies. Authors: Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, Delaporte M, Xu K, Aubertin AM, Moog C J. Virol., 2011;85(2):1077-85. Species: Human Sample Type: Whole Cells Application: Bioassay Lung-derived mesenchymal stromal cell post-transplantation survival, persistence, paracrine expression, and repair of elastase-injured lung. Authors: Hoffman AM, Paxson JA, Mazan MR, Davis AM, Tyagi S, Murthy S, Ingenito EP Stem Cells Dev., 2011;20(10):1779-92. Species: Mouse Sample Type: Whole Cells Application: Bioassay Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells. Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H J. Invest. Dermatol., 2011;131(10):2049-57. Species: Human Sample Type: Whole Cells Application: Bioassay Glycotranscriptome study reveals an enzymatic switch modulating glycosaminoglycan synthesis during B-cell development and activation. Authors: Duchez S, Pascal V, Cogne N, Jayat-Vignoles C, Julien R, Cogne M Eur. J. Immunol., 2011;41(12):3632-44. Species: Mouse Sample Type: Whole Cells Application: Bioassay An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ Mol. Biol. Cell, 2011;22(10):1686-98. Species: Canine Sample Type: Whole Cells Application: Bioassay Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy. Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L J. Exp. Med., 2011;208(3):491-503. Species: Mouse Sample Type: Whole Cells Application: Bioassay NFIL3/E4BP4 controls type 2 T helper cell cytokine expression. Authors: Kashiwada M, Cassel SL, Colgan JD EMBO J., 2011;30(10):2071-82. Species: Mouse Sample Type: Whole Cells Application: Bioassay Effects of high mobility group box protein-1, interleukin-1beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures. Authors: Ley C, Svala E, Nilton A, Lindahl A, Eloranta ML, Ekman S, Skioldebrand E Connect. Tissue Res., 2011;52(4):290-300. Species: Equine Sample Type: Whole Cells Application: Bioassay Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Authors: Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H Cell, 2011;147(5):1146-58. Species: Mouse Sample Type: In Vivo Application: In Vivo Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers. Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL Blood, 2011;117(19):5092-101. Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer. Authors: Zheng L, Foley K, Huang L, Leubner A, Mo G, Olino K, Edil BH, Mizuma M, Sharma R, Le DT, Anders RA, Illei PB, Van Eyk JE, Maitra A, Laheru D, Jaffee EM PLoS ONE, 2011;6(4):e19390. Species: Human Sample Type: Whole Cells Application: Bioassay Antigen-specific Th9 cells exhibit uniqueness in their kinetics of cytokine production and short retention at the inflammatory site. Authors: Tan C, Aziz MK, Lovaas JD, Vistica BP, Shi G, Wawrousek EF, Gery I J. Immunol., 2010;185(11):6795-801. Species: Human Sample Type: Whole Cells Application: Bioassay Expression of the HGF receptor c-met by macrophages in experimental autoimmune encephalomyelitis. Authors: Moransard M, Sawitzky M, Fontana A, Suter T Glia, 2010;58(5):559-71. Species: Mouse Sample Type: Whole Cells Application: Bioassay Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model. Authors: Yoshizaki A, Yanaba K, Iwata Y J. Immunol., 2010;185(4):2502-15. Species: Mouse Sample Type: Whole Cells Application: Bioassay The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation. Authors: Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH Nat. Immunol., 2010;11(6):527-34. Species: Mouse Sample Type: Whole Cells Application: Bioassay Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells. Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA J. Immunol., 2010;184(4):2130-9. Species: Mouse Sample Type: Whole Cells Application: Bioassay Regulation of the IL-21 gene by the NF-kappaB transcription factor c-Rel. Authors: Chen G, Hardy K, Bunting K J. Immunol., 2010;185(4):2350-9. Species: Mouse Sample Type: Whole Cells Application: Bioassay Recombinant expression, purification, and functional characterisation of connective tissue growth factor and nephroblastoma-overexpressed protein. Authors: Bohr W, Kupper M, Hoffmann K, Weiskirchen R PLoS ONE, 2010;5(12):e16000. Species: Human Sample Type: Whole Cells Application: Bioassay Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Authors: Jindra C Eur J Immunol, 2010;40(2):321-9. Species: Human Sample Type: Whole Cells Application: Bioassay Identification of retinoic acid in a high content screen for agents that overcome the anti-myogenic effect of TGF-beta-1. Authors: Krueger C, Hoffmann FM PLoS ONE, 2010;5(11):e15511. Species: Mouse Sample Type: Whole Cells Application: Bioassay The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Authors: Suzuki K, Maruya M, Kawamoto S Immunity, 2010;33(1):71-83. Species: Mouse Sample Type: Whole Cells Application: Bioassay Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis. Authors: Rai D, Kim SW, McKeller MR Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6. Species: Human Sample Type: Whole Cells Application: Bioassay TIM-3: a novel regulatory molecule of alloimmune activation. Authors: Boenisch O, D'Addio F, Watanabe T, Elyaman W, Magee CN, Yeung MY, Padera RF, Rodig SJ, Murayama T, Tanaka K, Yuan X, Ueno T, Jurisch A, Mfarrej B, Akiba H, Yagita H, Najafian N J. Immunol., 2010;185(10):5806-19. Species: Mouse Sample Type: Whole Cells Application: Bioassay Nuclear function of Smad7 promotes myogenesis. Authors: Miyake T, Alli NS, McDermott JC Mol. Cell. Biol., 2010;30(3):722-35. Species: Mouse Sample Type: Whole Cells Application: Bioassay Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems. Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB PLoS ONE, 2010;5(9):e13002. Species: Human Sample Type: Whole Cells Application: Bioassay Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid. Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL J. Invest. Dermatol., 2010;130(5):1297-306. Species: Human Sample Type: Whole Cells Application: Bioassay Transforming growth factor beta1 inhibits cystic fibrosis transmembrane conductance regulator-dependent cAMP-stimulated alveolar epithelial fluid transport via a phosphatidylinositol 3-kinase-dependent mechanism. Authors: Roux J, Carles M, Koh H, Goolaerts A, Ganter MT, Chesebro BB, Howard M, Houseman BT, Finkbeiner W, Shokat KM, Paquet AC, Matthay MA, Pittet JF J. Biol. Chem., 2010;285(7):4278-90. Species: Rat Sample Type: Whole Cells Application: Bioassay Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ Nat. Med., 2010;16(2):191-7. Species: Mouse Sample Type: Whole Cells Application: Bioassay Regulating human Th17 cells via differential expression of IL-1 receptor. Authors: Lee WW, Kang SW, Choi J Blood, 2010;115(3):530-40. Species: Human Sample Type: Whole Cells Application: Bioassay Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus. Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V J. Exp. Med., 2009;206(4):793-805. Species: Human Sample Type: Whole Cells Application: Bioassay Role of insulin-like growth factor binding protein-3 in allergic airway remodeling. Authors: Veraldi KL, Gibson BT, Yasuoka H, Myerburg MM, Kelly EA, Balzar S, Jarjour NN, Pilewski JM, Wenzel SE, Feghali-Bostwick CA Am. J. Respir. Crit. Care Med., 2009;180(7):611-7. Species: Human Sample Type: Whole Cells Application: Bioassay Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg. Authors: Stockis J, Colau D, Coulie PG, Lucas S Eur. J. Immunol., 2009;39(12):3315-22. Species: Human Sample Type: Whole Cells Application: Bioassay Glial cell type-specific subcellular localization of 14-3-3 zeta: an implication for JCV tropism. Authors: Lamba S, Ravichandran V, Major EO Glia, 2009;57(9):971-7. Species: Human Sample Type: Whole Cells Application: Bioassay CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1. Authors: Han Y, Guo Q, Zhang M, Chen Z, Cao X J. Immunol., 2009;182(1):111-20. Species: Mouse Sample Type: Whole Cells Application: Bioassay Activation of Toll-like receptor 3 augments myofibroblast differentiation. Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M Am. J. Respir. Cell Mol. Biol., 2009;40(6):654-62. Species: Human Sample Type: Whole Cells Application: Bioassay Growth factor regulation of growth factors in articular chondrocytes. Authors: Shi S, Mercer S, Eckert GJ, Trippel SB J. Biol. Chem., 2009;284(11):6697-704. Species: Human Sample Type: Whole Cells Application: Bioassay Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Nat. Immunol., 2009;10(8):857-63. Species: Human Sample Type: Whole Cells Application: Bioassay Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Circulation, 2009;119(10):1424-32. Species: Human Sample Type: Whole Cells Application: Bioassay Mast cell-dependent contraction of human airway smooth muscle cell-containing collagen gels: influence of cytokines, matrix metalloproteases, and serine proteases. Authors: Margulis A, Nocka KH, Brennan AM, Deng B, Fleming M, Goldman SJ, Kasaian MT J. Immunol., 2009;183(3):1739-50. Species: Human Sample Type: Whole Cells Application: Bioassay TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Th17 polarization of naive T cells but do stimulate Th1 and Th17 memory responses. Authors: Duraisingham SS, Hornig J, Gotch F, Patterson S J. Immunol., 2009;183(4):2242-51. Species: Human Sample Type: Whole Cells Application: Bioassay Imatinib mesylate and AMN107 inhibit PDGF-signaling in orbital fibroblasts: a potential treatment for Graves' ophthalmopathy. Authors: van Steensel L, Paridaens D, Schrijver B, Dingjan GM, van Daele PL, van Hagen PM, van den Bosch WA, Drexhage HA, Hooijkaas H, Dik WA Invest. Ophthalmol. Vis. Sci., 2009;50(7):3091-8. Species: Human Sample Type: Whole Cells Application: Bioassay TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M Eur. J. Immunol., 2009;39(5):1221-30. Species: Human Sample Type: Whole Cells Application: Bioassay Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P Int. J. Cancer, 2009;125(2):367-73. Species: Human Sample Type: Whole Cells Application: Bioassay Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment. Authors: Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, Fortugno P, Gonzalvo-Feo S, Franssen JD, Parmentier M, De Pita O, Girolomoni G, Sozzani S J. Exp. Med., 2009;206(1):249-58. Species: Human Sample Type: Whole Cells Application: Bioassay Role of Tim-3/galectin-9 inhibitory interaction in viral-induced immunopathology: shifting the balance toward regulators. Authors: Sehrawat S, Suryawanshi A, Hirashima M, Rouse BT J. Immunol., 2009;182(5):3191-201. Species: Mouse Sample Type: Whole Cells Application: Bioassay Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling. Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R J. Exp. Med., 2009;206(3):535-48. Species: Mouse Sample Type: Whole Cells Application: Bioassay Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. Authors: Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Kawasaki J, Vu AT, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H J. Allergy Clin. Immunol., 2009;123(1):179-86. Species: Human Sample Type: Whole Cells Application: Bioassay GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation. Authors: Nolan-Stevaux O, Lau J, Truitt ML, Chu GC, Hebrok M, Fernandez-Zapico ME, Hanahan D Genes Dev., 2009;23(1):24-36. Species: Mouse Sample Type: Whole Cells Application: Bioassay Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Authors: Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C Bone, 2009;44(6):1143-50. Species: Human Sample Type: Whole Cells Application: Bioassay Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling. Authors: Pedroja BS, Kang LE, Imas AO, Carmeliet P, Bernstein AM J. Biol. Chem., 2009;284(0):20708. Species: Mouse Sample Type: Whole Cells Application: Bioassay Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues. Authors: Vitsky A, Waire J, Pawliuk R, Bond A, Matthews D, Lacasse E, Hawes ML, Nelson C, Richards S, Piepenhagen PA, Garman RD, Andrews L, Thurberg BL, Lonning S, Ledbetter S, Ruzek MC Am. J. Pathol., 2009;174(6):2137-49. Species: N/A Sample Type: N/A Application: WB Ctrl Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection. Authors: Bhowmick S, Mazumdar T, Ali N Infect. Immun., 2009;77(4):1514-23. Species: Mouse Sample Type: In Vivo Application: In Vivo Transcriptional regulation of SM22alpha by Wnt3a: convergence with TGFbeta(1)/Smad signaling at a novel regulatory element. Authors: Shafer SL, Towler DA J. Mol. Cell. Cardiol., 2009;46(5):621-35. Species: Mouse Sample Type: Whole Cells Application: Bioassay Both Th1 and Th17 are immunopathogenic but differ in other key biological activities. Authors: Cox CA, Shi G, Yin H, Vistica BP, Wawrousek EF, Chan CC, Gery I J. Immunol., 2008;180(11):7414-22. Species: Mouse Sample Type: Whole Cells Application: Bioassay Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Authors: Shinohara ML, Kim JH, Garcia VA, Cantor H Immunity, 2008;29(1):68-78. Species: Mouse Sample Type: Whole Cells Application: Bioassay Transforming growth factor-beta stimulates intestinal epithelial focal adhesion kinase synthesis via smad- and p38-dependent mechanisms. Authors: Walsh MF, Ampasala DR, Hatfield J, Vander Heide R, Suer S, Rishi AK, Basson MD Am. J. Pathol., 2008;173(2):385-99. Species: Rat Sample Type: Whole Cells Application: Bioassay Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation. Authors: Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW J. Immunol., 2008;181(2):1103-8. Species: Human Sample Type: Whole Cells Application: Bioassay Induction of osteoclast-associated receptor, a key osteoclast costimulation molecule, in rheumatoid arthritis. Authors: Herman S, Muller RB, Kronke G, Zwerina J, Redlich K, Hueber AJ, Gelse H, Neumann E, Muller-Ladner U, Schett G Arthritis Rheum., 2008;58(10):3041-50. Species: Human Sample Type: Whole Cells Application: Bioassay Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha. Authors: Tsoporis JN, Izhar S, Parker TG J. Biol. Chem., 2008;283(44):30174-83. Species: Rat Sample Type: Whole Cells Application: Bioassay Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. Authors: Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP J. Allergy Clin. Immunol., 2008;121(6):1385-92, 1392. Species: Human Sample Type: Whole Cells Application: Bioassay Myogenic growth factors can decrease extraocular muscle force generation: a potential biological approach to the treatment of strabismus. Authors: Anderson BC, Christiansen SP, McLoon LK Invest. Ophthalmol. Vis. Sci., 2008;49(1):221-9. Species: Rabbit Sample Type: In Vivo Application: In Vivo Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: Whole Cells Application: Bioassay Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway. Authors: Aoudjehane L, Pissaia A, Scatton O, Podevin P, Massault PP, Chouzenoux S, Soubrane O, Calmus Y, Conti F Lab. Invest., 2008;88(9):973-85. Species: Human Sample Type: Whole Cells Application: Bioassay Specific signals involved in the long-term maintenance of radiation-induced fibrogenic differentiation: a role for CCN2 and low concentration of TGF-beta1. Authors: Haydont V, Riser BL, Aigueperse J, Vozenin-Brotons MC Am. J. Physiol., Cell Physiol., 2008;294(6):C1332-41. Species: Human Sample Type: Whole Cells Application: Bioassay alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation. Authors: Marsh D, Dickinson S, Neill GW, Marshall JF, Hart IR, Thomas GJ Cancer Res., 2008;68(9):3295-303. Species: Human Sample Type: Whole Cells Application: Bioassay A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs. Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A Arthritis Rheum., 2008;58(11):3510-9. Species: Mouse Sample Type: Whole Cells Application: Bioassay The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. Authors: Guo B, Chang EY, Cheng G J. Clin. Invest., 2008;118(5):1680-90. Species: Mouse Sample Type: Whole Cells Application: Bioassay Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis. Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H Invest. Ophthalmol. Vis. Sci., 2008;49(9):3999-4007. Species: Rat Sample Type: Aqueous humor Application: Bioassay The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH Stem Cells, 2008;26(4):1047-55. Species: Rat Sample Type: Tissue Homogenates Application: ELISA development Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types. Authors: Reed SA, Johnson SE J. Cell. Physiol., 2008;215(2):329-36. Species: Equine Sample Type: Whole Cells Application: Bioassay Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling. Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB Oncogene, 2008;27(54):6834-44. Species: Human Sample Type: Whole Cells Application: Bioassay Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells. Authors: Carlson ME, Hsu M, Conboy IM Nature, 2008;454(7203):528-32. Species: Mouse Sample Type: Whole Cells Application: Bioassay Fibrogenic fibroblasts increase intercellular adhesion strength by reinforcing individual OB-cadherin bonds. Authors: Pittet P, Lee K, Kulik AJ, Meister JJ, Hinz B J. Cell. Sci., 2008;121(0):877-86. Species: Rat Sample Type: Whole Cells Application: Bioassay Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity. Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH FEBS Lett., 2008;582(10):1451-8. Species: Mouse Sample Type: Whole Cells Application: Bioassay Deficient TACI expression on B lymphocytes of newborn mice leads to defective Ig secretion in response to BAFF or APRIL. Authors: Kanswal S, Katsenelson N, Selvapandiyan A, Bram RJ, Akkoyunlu M J. Immunol., 2008;181(2):976-90. Species: Mouse Sample Type: Whole Cells Application: Bioassay Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism. Authors: Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM Cancer Res., 2008;68(2):561-70. Species: Human Sample Type: Whole Cells Application: Bioassay In vivo investigations on anti-fibrotic potential of proteasome inhibition in lung and skin fibrosis. Authors: Fineschi S, Bongiovanni M, Donati Y, Djaafar S, Naso F, Goffin L, Argiroffo CB, Pache JC, Dayer JM, Ferrari-Lacraz S, Chizzolini C Am. J. Respir. Cell Mol. Biol., 2008;39(4):458-65. Species: Mouse Sample Type: Whole Cells Application: Bioassay Angiopoietin-like 7 secretion is induced by glaucoma stimuli and its concentration is elevated in glaucomatous aqueous humor. Authors: Kuchtey J, Kallberg ME, Gelatt KN, Rinkoski T, Komaromy AM, Kuchtey RW Invest. Ophthalmol. Vis. Sci., 2008;49(8):3438-48. Species: Human Sample Type: Whole Cells Application: Bioassay TLR3- and Th2 cytokine-dependent production of thymic stromal lymphopoietin in human airway epithelial cells. Authors: Kato A, Favoreto S, Avila PC, Schleimer RP J. Immunol., 2007;179(2):1080-7. Species: Human Sample Type: Whole Cells Application: Bioassay Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R J. Biol. Chem., 2007;282(32):23337-47. Species: Mouse Sample Type: Whole Cells Application: Bioassay Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis. Authors: Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM Arthritis Rheum., 2007;56(7):2432-42. Species: Human Sample Type: Whole Cells Application: Bioassay Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death. Authors: Toscano MA, Bianco GA, Ilarregui JM, Croci DO, Correale J, Hernandez JD, Zwirner NW, Poirier F, Riley EM, Baum LG, Rabinovich GA Nat. Immunol., 2007;8(8):825-34. Species: Mouse Sample Type: Whole Cells Application: Bioassay Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells. Authors: Tacke F, Gabele E, Bataille F, Schwabe RF, Hellerbrand C, Klebl F, Straub RH, Luedde T, Manns MP, Trautwein C, Brenner DA, Scholmerich J, Schnabl B Dig. Dis. Sci., 2007;52(12):3404-15. Species: Human Sample Type: Whole Cells Application: Bioassay Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes. Authors: Lamar JM, Iyer V, DiPersio CM J. Invest. Dermatol., 2007;128(3):575-86. Species: Mouse Sample Type: Whole Cells Application: Bioassay Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Whole Cells Application: Bioassay Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression. Authors: Brechter AB, Lerner UH Arthritis Rheum., 2007;56(3):910-23. Species: Human Sample Type: Whole Cells Application: Bioassay Essential role of Smad3 in infarct healing and in the pathogenesis of cardiac remodeling. Authors: Bujak M, Ren G, Kweon HJ, Dobaczewski M, Reddy A, Taffet G, Wang XF, Frangogiannis NG Circulation, 2007;116(19):2127-38. Species: Mouse Sample Type: Whole Cells Application: Bioassay The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension. Authors: Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE Eur. Respir. J., 2007;29(6):1094-104. Species: Rat Sample Type: Whole Cells Application: Bioassay A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J Blood, 2007;109(5):2234-42. Species: Mouse Sample Type: Cell Culture Supernates Application: Bioassay Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy? Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE Kidney Int., 2007;72(4):481-8. Species: Human Sample Type: Whole Cells Application: Bioassay Microsatellite unstable colorectal cancer cell lines with truncating TGFbetaRII mutations remain sensitive to endogenous TGFbeta. Authors: Baker K, Raut P, Jass JR J. Pathol., 2007;213(3):257-65. Species: Human Sample Type: Whole Cells Application: Bioassay Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH J. Immunol., 2007;179(6):3724-33. Species: Human Sample Type: Whole Cells Application: Bioassay IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. Authors: Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA J. Immunol., 2007;178(4):2018-27. Species: Mouse Sample Type: Whole Cells Application: Bioassay Crucial role of the protein C pathway in governing microvascular inflammation in inflammatory bowel disease. Authors: Scaldaferri F, Sans M, Vetrano S, Graziani C, De Cristofaro R, Gerlitz B, Repici A, Arena V, Malesci A, Panes J, Grinnell BW, Danese S J. Clin. Invest., 2007;117(7):1951-60. Species: Human Sample Type: Whole Cells Application: Bioassay Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling. Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ Am. J. Respir. Cell Mol. Biol., 2007;38(1):95-104. Species: Human Sample Type: Whole Cells Application: Bioassay Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium. Authors: Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW Cancer Res., 2007;67(9):4244-53. Species: Human Sample Type: Whole Cells Application: Bioassay Cyclic stretch controls the expression of CD40 in endothelial cells by changing their transforming growth factor-beta1 response. Authors: Korff T, Aufgebauer K, Hecker M Circulation, 2007;116(20):2288-97. Species: Human Sample Type: Whole Cells Application: Bioassay Antitumor activity of ALK1 in pancreatic carcinoma cells. Authors: Ungefroren H, Schniewind B, Groth S, Chen WB, Muerkoster SS, Kalthoff H, Fandrich F Int. J. Cancer, 2007;120(8):1641-51. Species: Human Sample Type: Whole Cells Application: Bioassay Induction of a distinct CD8 Tnc17 subset by transforming growth factor-beta and interleukin-6. Authors: Liu SJ, Tsai JP, Shen CR, Sher YP, Hsieh CL, Yeh YC, Chou AH, Chang SR, Hsiao KN, Yu FW, Chen HW J. Leukoc. Biol., 2007;82(2):354-60. Species: Mouse Sample Type: Whole Cells Application: Bioassay Bone response and mechanical strength of rabbit femoral defects filled with injectable CaP cements containing TGF-beta 1 loaded gelatin microparticles. Authors: Link DP, van den Dolder J, van den Beucken JJ, Wolke JG, Mikos AG, Jansen JA Biomaterials, 2007;29(6):675-82. Species: Rabbit Sample Type: In Vivo Application: In Vivo High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin. Authors: Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, Eling TE, Childs R, Ganz T, Leitman SF, Fucharoen S, Miller JL Nat. Med., 2007;13(9):1096-101. Species: N/A Sample Type: N/A Application: WB Ctrl TGF-beta and IL-6 drive the production of IL-17 and IL-10 by T cells and restrain T(H)-17 cell-mediated pathology. Authors: McGeachy MJ, Bak-Jensen KS, Chen Y, Tato CM, Blumenschein W, McClanahan T, Cua DJ Nat. Immunol., 2007;8(12):1390-7. Species: Mouse Sample Type: Whole Cells Application: Bioassay Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma. Authors: Hong KM, Belperio JA, Keane MP, Burdick MD, Strieter RM J. Biol. Chem., 2007;282(31):22910-20. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: N/A Sample Type: N/A Application: ELISA Standard Regulation of SDF-1 (CXCL12) production by osteoblasts; a possible mechanism for stem cell homing. Authors: Jung Y, Wang J, Schneider A, Sun YX, Koh-Paige AJ, Osman NI, McCauley LK, Taichman RS Bone, 2006;38(4):497-508. Species: Human Sample Type: Whole Cells Application: Bioassay Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS Exp. Cell Res., 2006;312(10):1909-23. Species: Human Sample Type: Whole Cells Application: Bioassay Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Authors: Bettelli E, Carrier Y, Gao W, Korn T, Strom TB, Oukka M, Weiner HL, Kuchroo VK Nature, 2006;441(7090):235-8. Species: Mouse Sample Type: Whole Cells Application: Bioassay IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R Eur. J. Immunol., 2006;36(5):1309-23. Species: Human Sample Type: Whole Cells Application: Bioassay The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Authors: Rhodes KE, Raivich G, Fawcett JW Neuroscience, 2006;140(1):87-100. Species: Rat Sample Type: In Vivo Application: In Vivo Coordinated functions of E-cadherin and transforming growth factor beta receptor II in vitro and in vivo. Authors: Andl CD, Fargnoli BB, Okawa T, Bowser M, Takaoka M, Nakagawa H, Klein-Szanto A, Hua X, Herlyn M, Rustgi AK Cancer Res., 2006;66(20):9878-85. Species: Human Sample Type: Whole Cells Application: Bioassay Transglutaminase 2 limits murine peritoneal acute gout-like inflammation by regulating macrophage clearance of apoptotic neutrophils. Authors: Rose DM, Sydlaske AD, Agha-Babakhani A, Johnson K, Terkeltaub R Arthritis Rheum., 2006;54(10):3363-71. Species: Mouse Sample Type: Whole Cells Application: Bioassay IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts. Authors: LÃƒÂ©tuvÃƒÂ© S, Lajoie-Kadoch S, Audusseau S, Rothenberg ME, Fiset PO, Ludwig MS, Hamid Q J. Allergy Clin. Immunol., 2006;117(3):590-6. Species: Human Sample Type: Whole Cells Application: Bioassay Wnt3a gradient converts radial to bilateral feather symmetry via topological arrangement of epithelia. Authors: Yue Z, Jiang TX, Widelitz RB, Chuong CM Proc. Natl. Acad. Sci. U.S.A., 2006;103(0):951-5. Species: Chicken Sample Type: In Vivo Application: In Vivo IGF-I and IGFBP-3 augment transforming growth factor-beta actions in human renal carcinoma cells. Authors: Rosendahl AH, Forsberg G Kidney Int., 2006;70(9):1584-90. Species: Human Sample Type: Whole Cells Application: Bioassay Transforming growth factor-beta1 sensitivity is altered in Abl-Myc- and Raf-Myc-induced mouse pre-B-cell tumors. Authors: Letterio J, Rudikoff E, Voong N, Bauer SR Stem Cells, 2006;24(12):2611-7. Species: Mouse Sample Type: Whole Cells Application: Bioassay Multipotent adult progenitor cells from swine bone marrow. Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C Stem Cells, 2006;24(11):2355-66. Species: Porcine Sample Type: Whole Cells Application: Bioassay The role of the activin system in keloid pathogenesis. Authors: Mukhopadhyay A, Chan SY, Lim IJ, Phillips DJ, Phan TT Am. J. Physiol., Cell Physiol., 2006;292(4):C1331-8. Species: Human Sample Type: Whole Cells Application: Bioassay Isolation of a novel population of multipotent adult stem cells from human hair follicles. Authors: Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M, Xu X Am. J. Pathol., 2006;168(6):1879-88. Species: Human Sample Type: Whole Cells Application: Bioassay Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure. Authors: Neurohr C, Nishimura SL, Sheppard D Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9. Species: Human Sample Type: Whole Cells Application: Bioassay Activin A functions as a Th2 cytokine in the promotion of the alternative activation of macrophages. Authors: Ogawa K, Funaba M, Chen Y, Tsujimoto M J. Immunol., 2006;177(10):6787-94. Species: Mouse Sample Type: Whole Cells Application: Bioassay Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. Authors: Li Y, Fan J, Chen M, Li W, Woodley DT J. Invest. Dermatol., 2006;126(9):2096-105. Species: Human Sample Type: Whole Cells Application: Bioassay Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ J. Cell. Physiol., 2005;203(1):35-43. Species: Human Sample Type: Whole Cells Application: Bioassay Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice. Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P Neurobiol. Dis., 2005;18(1):193-205. Species: Mouse Sample Type: In Vivo Application: In Vivo Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma. Authors: Pound JD, Batth BK, Johannessen I, Wood K J. Immunol., 2005;174(5):3015-23. Species: Human Sample Type: Whole Cells Application: Bioassay The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts. Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N J. Allergy Clin. Immunol., 2005;117(1):103-10. Species: Human Sample Type: Whole Cells Application: Bioassay CYP7B expression and activity in fibroblast-like synoviocytes from patients with rheumatoid arthritis: regulation by proinflammatory cytokines. Authors: Dulos J, van der Vleuten MA, Kavelaars A, Heijnen CJ, Boots AM Arthritis Rheum., 2005;52(3):770-8. Species: Human Sample Type: Whole Cells Application: Bioassay Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing. Authors: Brenner N, Huning M, Stange R Calcif. Tissue Int., 2004;74(4):388-97. Species: Rat Sample Type: In Vivo Application: In Vivo Transforming growth factor beta1 expression and activation is increased in the alcoholic rat lung. Authors: Bechara RI, Brown LA, Roman J, Joshi PC, Guidot DM Am. J. Respir. Crit. Care Med., 2004;170(2):188-94. Species: Mink Sample Type: Cell Lysates Application: Bioassay Local delivery of growth factors from coated titanium plates increases osteotomy healing in rats. Authors: Wildemann B, Bamdad P, Holmer Ch, Haas NP, Raschke M, Schmidmaier G Bone, 2004;34(5):862-8. Species: Rat Sample Type: In Vivo Application: In Vivo Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Authors: Phillips TM Electrophoresis, 2004;25(10):1652-9. Species: N/A Sample Type: N/A Application: Standard IL-22 increases the innate immunity of tissues. Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R Immunity, 2004;21(2):241-54. Species: Human Sample Type: Whole Cells Application: Bioassay Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10. Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA J. Immunol., 2004;172(9):5213-21. Species: Human Sample Type: Whole Cells Application: Bioassay Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Dev. Biol., 2004;266(2):223-37. Species: Human Sample Type: Whole Cells Application: Bioassay Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition. Authors: Studer RK, Bergman R, Stubbs T, Decker K Arthritis Res. Ther., 2004;6(1):R56-R64. Species: Rabbit Sample Type: Whole Cells Application: Bioassay Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates. Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ J. Immunol., 2004;172(9):5753-64. Species: N/A Sample Type: N/A Application: WB Ctrl Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts. Authors: Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J Arthritis Rheum., 2004;50(8):2521-30. Species: Human Sample Type: Whole Cells Application: Bioassay Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. Authors: Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T J. Clin. Invest., 2003;111(9):1309-18. Species: Mouse Sample Type: In Vivo Application: In Vivo Dual roles of IL-4 in lung injury and fibrosis. Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH J. Immunol., 2003;170(4):2083-92. Species: Mouse Sample Type: Whole Cells Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3. Authors: Chen, WanJun, Jin, Wenwen, Hardegen, Neil, Lei, Ke-Jian, Li, Li, Marinos, Nancy, McGrady, George, Wahl, Sharon M J Exp Med, 2003;198(12):1875-86. Species: Mouse Sample Type: Whole Cells Application: Bioassay An essential role for Scurfin in CD4+CD25+ T regulatory cells. Authors: Khattri, Roli, Cox, Tom, Yasayko, Sue-Ann, Ramsdell, Fred Nat Immunol, 2003;4(4):337-42. Species: Mouse Sample Type: Whole Cells Application: Bioassay Induction of a hypertrophic growth status of coronary smooth muscle cells is associated with an overexpression of TGF-beta. Authors: Schmidt A, Gopfert C, Vlodavsky I, Volker W, Buddecke E Eur. J. Cell Biol., 2002;81(3):138-44. Species: Bovine Sample Type: Whole Cells Application: Bioassay Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56. Species: N/A Sample Type: N/A Application: ELISA Standard Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-beta receptor type II. Authors: Zhao W, Kobayashi M, Ding W, Yuan L, Seth P, Cornain S, Wang J, Okada F, Hosokawa M Cancer Immunol. Immunother., 2002;51(7):381-8. Species: Rat Sample Type: Whole Cells Application: Bioassay Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways. Authors: Rosado E, Schwartz Z, Sylvia VL, Dean DD, Boyan BD Biochim. Biophys. Acta, 2002;1590(1):1-15. Species: Rat Sample Type: Whole Cells Application: Bioassay Loss of transforming growth factor counteraction on interleukin 1 mediated effects in cartilage of old mice. Authors: Scharstuhl A, van Beuningen HM, Vitters EL, van der Kraan PM, van den Berg WB Ann. Rheum. Dis., 2002;61(12):1095-8. Species: Mouse Sample Type: In Vivo Application: In Vivo A profibrotic function of IL-12p40 in experimental pulmonary fibrosis. Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D J. Immunol., 2002;169(5):2653-61. Species: Mouse Sample Type: Whole Cells Application: Bioassay Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C Blood, 2002;99(3):792-9. Species: Human Sample Type: Whole Cells Application: Bioassay Growth factors stimulate neointimal cells in vitro and increase the thickness of the neointima formed at the neck of porcine aneurysms treated by embolization. Authors: Desfaits AC, Raymond J, Muizelaar JP Stroke, 2000;31(0):498-507. Species: Canine Sample Type: In Vivo Application: In Vivo Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4. Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL J. Immunol., 2000;164(10):5337-43. Species: N/A Sample Type: N/A Application: ELISA Standard Isoform specificity of commercially-available anti-TGF-beta antibodies. Authors: Mozes MM, Hodics T, Kopp JB J. Immunol. Methods, 1999;225(1):87-93. Species: N/A Sample Type: N/A Application: WB Ctrl Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I. Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM Cancer Res., 1998;58(17):4008-14. Species: Human Sample Type: Whole Cells Application: Bioassay Identification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1. Authors: Villegas V, Rahman M, Fernandez-Barrena M, Diao Y, Liapi E, Sonkoly E, Stahle M, Pivarcsi A, Annaratone L, Sapino A, Ramirez Clavijo S, Burglin T, Shimokawa T, Ramachandran S, Kapranov P, Fernandez-Zapico M, Zaphiropoulos P Mol Oncol, 214;8(5):912-26. Species: Human Sample Type: Whole Cells Application: Bioassay Human macrophages induce CD4(+)Foxp3(+) regulatory T cells via binding and re-release of TGF-beta. Authors: Schmidt A, Zhang X, Joshi R, Iqbal S, Wahlund C, Gabrielsson S, Harris R, Tegner J Immunol Cell Biol, 0;94(8):747-62. Species: Human Sample Type: Whole Cells Application: Bioassay Comparative Analysis of Protocols to Induce Human CD4+Foxp3+ Regulatory T Cells by Combinations of IL-2, TGF-beta, Retinoic Acid, Rapamycin and Butyrate. Authors: Schmidt A, Eriksson M, Shang M, Weyd H, Tegner J PLoS ONE, 0;11(2):e0148474. Species: Human Sample Type: Whole Cells Application: Bioassay Expand to show all 361 Citations

Animal Component Free Recombinant Proteins

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Recombinant Human TGF-beta 1, ACFP Protein

BA ACFP240

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Average Rating: 4.6 (Based on 13 reviews)

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Read what people are saying who have used Recombinant Human TGF-beta 1 Protein.

We have 13 reviews tested in 8 applications: Media additive for protein or antibody production, Stem/Immune cell maintenance or differentiation , T cell differentiation, fibroblast-to-myofibroblast differentiation, In vitro bioactivity in cell culture, Cell proliferation, Cell migration/motility, we use it to prepare Metsr medium..

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Media additive for protein or antibody production Recombinant Human TGF-beta 1 Protein 240-B

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Excellent

Media additive for protein or antibody production

Anonymous

05/17/2017

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Media additive for protein or antibody production: Recombinant Human TGF-beta 1 Protein [240-B]

Summary

Application	Media additive for protein or antibody production
Reason for Rating	TGFbeta was used to understand EMT process in PANC1 cells and upon treatment we detected classic EMT as anticipated

Other Experimental Details

EMT was induced in PANC1 using TGF beta. EMT markers at both protein and mRNA level were examined using RT PCR and WB. Typical markers of EMT was seen to be altered after TGFbeta treatment in accordance to the desired effect which is induction of EMT

Stem/Immune cell maintenance or differentiation Recombinant Human TGF-beta 1 Protein 240-B

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Excellent

Stem/Immune cell maintenance or differentiation

Anonymous

05/02/2017

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Stem/Immune cell maintenance or differentiation : Recombinant Human TGF-beta 1 Protein [240-B]

Summary

Application	Stem/Immune cell maintenance or differentiation
Reason for Rating	Very good and reproducible performance across experiments, lots, and primary T cell donors.Note: Storage conditions are important and product needs to be used within a specified time.

Other Experimental Details

Product used for differentiation of human FOXP3+ cells (regulatory T cells, Tregs) from naive CD4+ T cells, within different experimental setups. You may add these papers to the references for this product. We name the product in these papers: Éliás S, Schmidt A, Kannan V, Andersson J, Tegnér J (2016): TGF-beta Affects the Differentiation of Human GM-CSF+ CD4+ T Cells in an Activation- and Sodium-Dependent Manner. Frontiers in Immunology 2016 Dec 23;7:603. doi: 10.3389/fimmu.2016.00603. PMID: 28066414. Schmidt A, Éliás S, Joshi RN, Tegnér J (2016): In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-beta -containing Protocol. Journal of Visualized Experiments 2016 Dec 30;(118). doi: 10.3791/55015. PMID: 28060341. Schmidt A, Zhang XM, Joshi RN, Iqbal S, Wahlund C, Gabrielsson S, Harris R, Tegnér J (2016): Human macrophages induce CD4+Foxp3+ regulatory T cells via binding and re-release of TGF-beta. Immunology & Cell Biology Sep;94(8):747-62. doi: 10.1038/icb.2016.34. PMID: 27075967.Schmidt A, Eriksson M, Shang MM, Weyd H, Tegnér J (2016): Comparative Analysis of Protocols to Induce Human CD4+Foxp3+ Regulatory T Cells by Combinations of IL‐2, TGF‐beta, Retinoic Acid, Rapamycin and Butyrate. PLoS ONE 11(2), e0148474. doi:10.1371/journal.pone.0148474. PMID: 26886923.

T cell differentiation Recombinant Human TGF-beta 1 Protein 240-B

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Excellent

T cell differentiation

Anonymous

04/14/2017

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T cell differentiation: Recombinant Human TGF-beta 1 Protein [240-B]

Summary

Application	T cell differentiation
Reason for Rating	Works well in inducing Foxp3 expression in naive mouse CD4 T cells.

Other Experimental Details

Naive mouse T cells were stimulated with anti-CD3e, anti-CD28, IL-2, and human TGF-beta. Four days later, Foxp3 induction was assessed by intracellular flow cytometry.

Very Good

fibroblast-to-myofibroblast differentiation

Anonymous

02/20/2017

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Summary

Application

fibroblast-to-myofibroblast differentiation

Other Experimental Details

TGF-beta stimulation of primary murine lung fibroblasts

Excellent

In vitro bioactivity in cell culture

Anonymous

08/08/2016

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Summary

Application	In vitro bioactivity in cell culture
Reason for Rating	consistent high quality protein that retains activity. Reliable.

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Excellent

Stem/Immune cell maintenance or differentiation

Anonymous

07/23/2016

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Stem/Immune cell maintenance or differentiation : Recombinant Human TGF-beta 1 Protein [240-B]

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Application

Stem/Immune cell maintenance or differentiation

In vitro bioactivity in cell culture Recombinant Human TGF-beta 1 Protein 240-B

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Very Good

In vitro bioactivity in cell culture

Anonymous

07/21/2016

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In vitro bioactivity in cell culture: Recombinant Human TGF-beta 1 Protein [240-B]

Summary

Application	In vitro bioactivity in cell culture
Reason for Rating	Saw morphology changes

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Excellent

In vitro bioactivity in cell culture

Anonymous

07/21/2016

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In vitro bioactivity in cell culture: Recombinant Human TGF-beta 1 Protein [240-B]

Summary

Application	In vitro bioactivity in cell culture
Reason for Rating	worked as published results

Excellent

Cell proliferation

Anonymous

05/12/2016

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Summary

Application

Cell proliferation

Very Good

Cell migration/motility

Anonymous

05/12/2016

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Summary

Application	Cell migration/motility
Reason for Rating	Used the product at 5ng/mL to induce EMT in primary human epithelial cells. Worked well at 48 hours post-induction with confirmation of EMT by microscopy as well as western for protein markers.

Very Good

Cell proliferation

Anonymous

05/12/2016

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Summary

Application

Cell proliferation

Excellent

we use it to prepare Metsr medium.

Anonymous

01/13/2016

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Summary

Application

we use it to prepare Metsr medium.

Very Good

In vitro bioactivity in cell culture

Anonymous

01/13/2016

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Summary

Application

In vitro bioactivity in cell culture

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Formulations

Size	Price	Qty	Stk
240-B-002/CF 2 ug	$259
240-B-002 2 ug	$259
240-B-010 10 ug	$809
240-B-010/CF 10 ug	$809
240-B-001MG/CF 1 mg	$13,500

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Size	Price	Qty	Stk
240-B-002/CF 2 ug	$259
240-B-002 2 ug	$259
240-B-010 10 ug	$809
240-B-010/CF 10 ug	$809
240-B-001MG/CF 1 mg	$13,500

Recombinant Human TGF-beta 1 Protein

Recombinant Human TGF-beta 1, ACFP Protein

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Recombinant Human TGF-beta 1 Protein

Recombinant Human TGF-beta 1, ACFP Protein

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