Recombinant Human VSIG3 His-tag Protein, CF

R&D Systems | Catalog # 10806-VS

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human VSIG3 His-tag Protein (10806-VS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q5DX21.3

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human VSIG3 protein
Leu23-Gly245, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

24 kDa

SDS-PAGE

32-41 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

Recombinant Human VSIG3 His-tag (Catalog # 10806-VS) binds to Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) with an ED50 of 0.0750 - 0.750 μg/mL.

Scientific Data Images for Recombinant Human VSIG3 His-tag Protein, CF

Recombinant Human VSIG3 His-tag Protein Binding Activity.

Recombinant Human VSIG3 His-tag Protein (Catalog # 10806-VS) binds to Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) with an ED50 of 0.0750 - 0.750 μg/mL.

Recombinant Human VSIG3 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human VSIG3 His-tag (Catalog # 10806-VS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-41 kDa.

Formulation, Preparation, and Storage

10806-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG3

VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is an approximately 50 kDa transmembrane adhesion protein (1). Mature human VSIG3 consists of a 219 amino acid (aa) extracellular domain (ECD) that contains two tandem Ig-like domains, a 21 aa transmembrane segment, and a 169 aa cytoplasmic domain (2). Within the ECD, human VSIG3 shares 95% aa sequence identity with mouse and rat VSIG3. Alternative splicing generates additional isoforms with a substituted signal peptide that may also have a deletion in the second Ig-like domain (3). VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (2-4). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (3-5). VSIG3 also localizes to neuronal postsynaptic densisties where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (6). The short isoform can be up-regulated in gastric cancer (7). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (8).

References

  1. Schreiber, J. et al. (2014) Adv. Neurobiol. 8:21.
  2. Suzu, S. et al. (2002) Biochem. Biophys. Res. Commun. 296:1215.
  3. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
  4. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
  5. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
  6. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
  7. Watanabe, T. et al. (2005) Cancer Sci. 96:498.
  8. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Alternate Names

BTIGSF, CXADRL1, IGSF11

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Gene Symbol

IGSF11

UniProt

Q5DX21.3

Additional VSIG3 Products

Product Documents for Recombinant Human VSIG3 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human VSIG3 His-tag Protein, CF

For research use only

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