Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7126-B7-050
7126-B7-01M
Recombinant Human VISTA/B7-H5/PD-1H  Fc Chimera Protein Bioactivity
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Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human VISTA/B7-H5/PD-1H protein
Human VISTA/B7-H5/PD-1H
(Phe33-Ala194)
Accession # AAH20568
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Phe33
Structure / Form
Disufide-linked homodimer
Predicted Molecular Mass
44.8 kDa (monomer)
SDS-PAGE
64-75 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7126-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human VISTA/B7-H5/PD-1H  Fc Chimera Protein Bioactivity View Larger

Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.

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Background: VISTA/B7-H5/PD-1H

VISTA (V-domain immunoglobulin suppressor of T cell activation) is an immune checkpoint receptor expressed primarily on the surface of hematopoietic cells. Specifically, VISTA is expressed on naïve CD4+ T cells and regulatory T cells (1). And it is up regulated in vivo on activated monocytes and dendritic cells (2). VISTA inhibits CD4+ and CD8+ T cell proliferation, and their production of the proinflammatory cytokines IL 2 and IFN gamma (1). VISTA protein is also observed on the surface of cancer cells and attenuates the anti tumor immune response enabling tumor progression (1). In the autoimmune disease model EAE, VISTA limits disease progression by downregulating the overactive immune system (1). VISTA, also known as B7 H5, PD-1H, Platelet receptor Gi24, Dies1, and SISP1, is a 55 65 kDa transmembrane glycoprotein with homology to B7 like immune co stimulatory molecules (3, 4). The mature human VISTA protein contains a 162 amino acid extracellular domain (ECD) with one V type Ig like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human VISTA shares 70% and 67% amino acid sequence identity with mouse and rat VISTA, respectively (5). The 30 kDa ECD can be shed by MT1 MMP, with a 25 30 kDa fragment remaining in the membrane (5). VISTA promotes both MT1 MMP expression and the MT1 MMP mediated activation of MMP 2 (5). VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP 4 induced signaling in ESC but is also down regulated following BMP 4 exposure (2, 6). It binds to BMP 4 directly and also associates with the type I BMP receptor Activin RIB/ALK 4 (2, 6). The identification of a binding partner for VISTA proved elusive for many years until two 2019 publications identified two distinct ligands for VISTA, VSIG-3 and PSGL-1 (7, 8). Both ligands are expressed on T cells and blocking antibodies to VISTA have been shown to reverse immune suppression making VISTA a potential therapeutic target for cancer (7, 8).

References
  1. Wang, L. et al. (2011) J. Exp. Med. 208:577. 
  2. Parisi, S. et al. (2012) FASEB J. 26:3957. 
  3. Flajnik, M.F. et al. (2012) Immunogenetics 64:571. 
  4. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465. 
  5. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368. 
  6. Aloia, L. et al. (2010) J. Biol. Chem. 285:7776. 
  7. Wang, J. et al. (2019) Immunology. 156:74. 
  8. Johnston, R.J. et al. (2019) Nature. 574:565.
Entrez Gene IDs
64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
Alternate Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR

Citation for Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein, CF
By Anonymous on 01/21/2016
Application: In vitro bioactivity in cell culture