Recombinant Human XCL2/SCM-1 beta Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
8418-X2-025/CF
Recombinant Human XCL2/SCM-1 beta Protein, CF Bioactivity
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Recombinant Human XCL2/SCM-1 beta Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human XCR1. The ED50 for this effecy is 10-60 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human XCL2/SCM-1 beta protein
Val22-Gly114
Accession #
N-terminal Sequence
Analysis
Val22
Predicted Molecular Mass
10 kDa
SDS-PAGE
19-22 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8418-X2/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8418-X2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

 

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Human XCL2/SCM-1 beta (Catalog # 8418-X2/CF) chemoattracts BaF3 mouse pro B cells transfected with human XCR1. The ED50for this effect is 10-60 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XCL2/SCM-1 beta

Human XCL2 (also known as Lymphotactin-2, SCYC2, and SCM-1 beta ) is a 10 kDa glycosylated protein in the C chemokine family (1-3). XCL1/Lymphotactin is a closely related chemokine that differs from XCL2 by only two amino acids (aa). Human XCL2 encodes a 114 amino acid aa precursor protein with a 21 aa predicted signal peptide. Members of the C chemokine family lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines. They also have an extended carboxy terminus (4). XCL1/Lymphotactin which is expressed in activated T cells and NK cells, while certain stimulatory conditions have been shown to induce XCL2 expression in macrophages (5-7). XCL2 and XCL1/Lymphotactin bind and activate the G protein-coupled receptor, XCR1. XCR1 is expressed on dendritic cells, T lymphocytes, and B lymphocytes (8-11). Binding of XCL2 to XCR1 initiates chemotaxic cell migration in dendritic cells as well as in T and B lymphocytes. XCR1 is also expressed on ovarian carcinoma cells and may contribute to cancer cell migration and proliferation in response to XCL2 or XCL1/Lymphotactin (12).

References
  1. Yoshida, T. et al. (1996) FEBS Lett. 395:82.
  2. Kelner, G.S. et al. (1994) Science 266:1395.
  3. Muller, S. et al. (1995) Eur. J. Immunol. 25:1744.
  4. Yoshida, T. et al. (1995) FEBS Lett. 360:155.
  5. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
  6. Luzi, G. et al. (2007) Clin. Immunol. 125:127.
  7. Cao, W. et al. (2008) Curr. Microbiol. 57:189.
  8. Kroczek, R.A. and V. Henn (2012) Front. Immunol. 3:14.
  9. Huang, H. et al. (2001) Biochem. Biophys. Res. Commun. 281:378.
  10. Pigard, N. et al. (2009) J. Neuroimmunol. 209:114.
  11. Yamazaki, C. et al. (2010) Biochem. Biophys. Res. Commun. 397:756.
  12. Kim, M. et al. (2012) Mol. Cancer. Res. 10:1419.
Long Name
XCL 2/SCM-1 beta
Entrez Gene IDs
6846 (Human)
Alternate Names
C motif chemokine 2; chemokine (C motif) ligand 2; cytokine SCM-1 beta; SCM1 beta; SCM-1 beta; SCM1B; SCM-1b; SCYC2; SCYC2XC chemokine ligand 2; small inducible cytokine subfamily C, member 2; XCL2

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