Recombinant Mouse BTNL4 His-tag Protein, CF
Recombinant Mouse BTNL4 His-tag Protein, CF Summary
Gln29-Trp250, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse BTNL4 (Catalog # 9594-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.8-4 μg/mL.
Butyrophilin-like 4, also known as BTLN4, BTN3A3 (human) and BTN3A2 (rat) is an approximately 65 kDa member of the butyrophilin family that may play a role in immune regulation (1-3). The mouse BTNL4 is a 586 aa type 1 transmembrane protein, consisting of a 28 aa signal peptide, followed by a 109 aa immunoglobulin (Ig) V domain, a 71 aa IgC domain, a 20 aa transmembrane domain, and a 170 aa cytoplasmic B30.2/SPRY domain (4). Within the extracellular domain including aa 29‑250, mouse BTNL4 shares 45% and 82% sequence identity with human BTN3A3 and rat BTN3A2, respectively. BTNL4 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL4 co-inhibited anti-CD3 induced IL‑2 secretion on CD3+ cells.
- Yamashiro, H. et al. (2010) J. Leukoc Biol. 88:757.
- Compte, E. et al. (2004) Eur. J. Immunol. 34:2089.
- Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
- Bas, A. et al. (2011) Proc Natl Acad Sci U S A. 108:4376.
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