Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

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Catalog # Availability Size / Price Qty
9638-BT-050
Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein Bioactivity
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Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.15-0.9 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived mouse BTNL6 protein
Lys29-Trp249, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Lys29
Predicted Molecular Mass
26 kDa
SDS-PAGE
28-38 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9638-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein Bioactivity View Larger

Recombinant Mouse BTNL6/Butyrophilin-like 6 (Catalog # 9638-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.15-0.9 μg/mL.

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Background: BTNL6

Butyrophilin-like 6 (BTNL6) is a member of the BTN/MOG Ig-superfamily and functions with BTNL1 as a regulator of immune cell proliferation (1). Mouse BTNL6 is a 539 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a cytoplasmic domain. The ECD features a V-type and a C-type Ig-like domain and one glycosylation site. The Btnl6 gene is found only in mice (2). The ECD of mouse BTNL6 (aa 29-249) shares 47% and 83% sequence identity with the ECD of human BTNL2A2 and rat BTNL9, respectively. BTNL6 expression is coordinated as a heteromeric protein with BTNL1, and the presence of this complex is correlated with expansion of gamma δ T cells, especially those containing V gamma 7Vδ4 TCR (2). Btnl6 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL6 co-inhibited anti-CD3 induced IL-2 secretion on CD3+ cells.

References
  1. Lebrero-Fernández C. et al. (2016) Front Immunol 7:1.
  2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:559.
  3. Lebrero-Fernández C and Bas-Forsberg A. (2016). Sci Rep. 6:31524.
  4. Bas, A. et al. (2011) Proc. Natl Acad. Sci. USA 108:4376.
Long Name
Butyrophilin-like 6
Entrez Gene IDs
624681 (Mouse)
Alternate Names
BTNL6; Gm6519; NG13

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