Recombinant Mouse Galectin-3 Protein, CF

Newer Version Available: 9039-GAB
Formulations:
Catalog # Availability Size / Price Qty
1197-GA-050/CF
1197-GA-01M/CF
Recombinant Mouse Galectin-3 Protein, CF Data
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Product Details
Citations (5)
FAQs
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Recombinant Mouse Galectin-3 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 5‑20 µg/mL.
Source
E. coli-derived mouse Galectin-3 protein
Ala2-Ile264
Accession #
N-terminal Sequence
Analysis
Ala2
Predicted Molecular Mass
27.3 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1197-GA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

1197-GA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Mouse Galectin-3 (Catalog # 1197-GA/CF) agglutinates human red blood cells. The ED50for this effect is 5‑20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature mouse Galectin-3 shares 80% and 86% amino acid (aa) sequence identity with human and rat Galectin-3, respectively. Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the cross-linking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

References
  1. Robertson, M.W. et al. (1990) Biochemistry 29:8093.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
  4. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
  5. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
  6. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
  7. Sano, H. et al. (2003) J. Clin. Invest. 112:389.
  8. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
  9. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
  10. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
  11. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
  12. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
  13. Kohatsu, L. et al. (2006) J. Immunol. 177:4718.
  14. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
  15. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
  16. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
  17. Sherwi, N. et al. (2012) Future Cardiol. 8:885.
  18. Shin, T. (2013) Acta Histochem. 115:407.
Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

Citations for Recombinant Mouse Galectin-3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Inhibition of galectin-3 reduces atherosclerosis in apolipoprotein E-deficient mice.
    Authors: Mackinnon, Alison C, Liu, Xiaojun, Hadoke, Patrick, Miller, Mark R, Newby, David E, Sethi, Tariq
    Glycobiology, 2013;23(6):654-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Galectin-3 accelerates M2 macrophage infiltration and angiogenesis in tumors.
    Authors: Jia W, Kidoya H, Yamakawa D, Naito H, Takakura N
    Am J Pathol, 2013;182(5):1821-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Galectin-3 is a negative regulator of lipopolysaccharide-mediated inflammation.
    Authors: Li Y, Komai-Koma M, Gilchrist DS, Hsu DK, Liu FT, Springall T, Xu D
    J. Immunol., 2008;181(4):2781-9.
    Species: N/A
    Sample Types: N/A
    Applications: Bioassay
  4. Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
    Authors: Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
    Am. J. Pathol., 2007;170(2):546-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Presentation of galectin-1 by extracellular matrix triggers T cell death.
    Authors: He J, Baum LG
    J. Biol. Chem., 2003;279(6):4705-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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