Recombinant Mouse IL-6/IL-6 R alpha Protein Chimera, CF

Formulations:
Catalog # Availability Size / Price Qty
9038-SR-025/CF
Recombinant Mouse IL-6/IL-6  R alpha Protein Chimera, CF  Bioactivity
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Recombinant Mouse IL-6/IL-6 R alpha Protein Chimera, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 2-12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-6/IL-6 R alpha Complex protein
Mouse IL-6 R alpha
(Leu20-Glu357)
Accession # P22272
GGGSGGGSGGGS Mouse IL-6
(Phe25-Thr211)
Accession # P08505
N-terminusC-terminus
N-terminal Sequence
Analysis
Leu20 (major), Leu28 (minor)
Predicted Molecular Mass
60 kDa
SDS-PAGE
70-87 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9038-SR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9038-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse IL-6/IL-6  R alpha Protein Chimera, CF  Bioactivity View Larger

Recombinant Mouse IL-6/
IL-6 R alpha complex (Catalog #
9038-SR/CF) inhibits M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 2‑12 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6/IL-6 R alpha Complex

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1-3). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 6). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (7). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (8). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (3, 9). Mature mouse IL-6 shares 39% and 85% amino acid (aa) sequence identity with human and rat IL-6, respectively (10-12). Within the extracellular domain, mouse IL-6 R alpha shares 51% and 89% aa sequence identity with human and rat IL-6 R alpha, respectively (13).

References
  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Hunter, C.A. and S.A. Jones (2015) Nat. Immunol. 16:448.
  4. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  5. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  6. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
  7. Murakami, M. et al. (1993) Science 260:1808.
  8. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  9. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  11. Simpson, R.J. et al. (1988) Eur. J. Biochem. 176:187.
  12. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
  13. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
Long Name
Interleukin 6/Interleukin 6 Receptor alpha Complex
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat)
Alternate Names
IL-6/IL-6 R alpha Complex

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