Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF

R&D Systems | Catalog # 9330-NK

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein (9330-NK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_034782

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all NKG2A/NKG2B Isoform 2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse NKG2A/NKG2B Isoform 2 protein
MDP Mouse IgG2A
(Glu98-Lys330)		 IEGR Mouse NKG2A
(Ala94-IIe227)
Accession # NP_034782
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

42 kDa

SDS-PAGE

57-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Chimera is coated at 0.5 µg/mL, 100 μL/well, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED50 of 0.25-1.5 μg/mL.

Scientific Data Images for Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF

Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein Bioactivity

Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein Bioactivity

When Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Chimera (Catalog # 9330-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED50 of 0.25-1.5 µg/mL.

Formulation, Preparation, and Storage

9330-NK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2A/NKG2B Isoform 2

NKG2B is a member of the C-type lectin superfamily of proteins.  It is a splice variant of NKG2A which has an additional 17 aa in the extracellular juxtamembrane region (1).  NKG2B is an approximately 40 kDa transmembrane protein, with 28 aa in the transmembrane region, and 105 aa in the extracellular domain (ECD).  Within the ECD, mouse NKG2B shares 40% and 67% aa identity with human and rat NKG2B, respectively. NKG2B is expressed on a subset of NK cells and CD8+ T cells (2-6) where it forms a covalent heterodimer with CD94 (5, 7, 8). NKG2A-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (2, 7). Triggering the NKG2A-CD94 complex inhibits the cytolytic activity of NK and CD8+ T cells (2, 3, 5, 6, 9). This enables the innate immune system to detect cells that express host MHC-I molecules and to protect them from NK cell mediated lysis. This mechanism is subverted by human cytomegalovirus which encodes a peptide that is homologous to the HLA-E binding peptide (10). HCMV infected cells up-regulate both HLA-E and NKG2A expression and utilize this peptide to escape from immune clearance (3, 10). In contrast, vaccinia virus induces HLA-E down-regulation, thus permitting NK cell lysis of the virally infected cell (11).

References

  1. Lohwasser S, et al. (1999) Eur J Immunol. 29:755.
  2. Vance, R.E. et al. (1998) J. Exp. Med. 188:1841.
  3. Saez-Borderias, A. et al. (2009) J. Immunol.182:829.
  4. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
  5. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
  6. Zhou, J. et al. (2008) J. Immunol. 180:25.
  7. Braud, V.M. et al. (1998) Nature 391:795.
  8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
  9. Lee, N. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5199.
  10. Tomasec, P. et al. (2000) Science 287:1031.
  11. Brooks, C.R. et al. (2006) J. Immunol. 176:1141.

Long Name

NKG2-A/NKG2-B Type II Integral Membrane Protein

Alternate Names

NKG2-A/B Type II Integral Membrane Protein

Entrez Gene IDs

3821 (Human); 16641 (Mouse)

Gene Symbol

KLRC1

UniProt

NP_034782

Additional NKG2A/NKG2B Isoform 2 Products

Product Documents for Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF

For research use only

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