Recombinant Human CD94 Protein, CF

R&D Systems | Catalog # 9270-CD

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD94 Protein (9270-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q13241

Applications

Bioactivity
View all CD94 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD94 protein
Lys32-Ile179, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys32

Predicted Molecular Mass

18 kDa

SDS-PAGE

21-38 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse NKG2A/CD159a Fc Chimera (Catalog # 9198-NK) is coated at 0.5 µg/mL, 100 μL/well, recombinant human CD94 binds with a typical ED50 of 0.1-0.6 μg/mL

Scientific Data Images for Recombinant Human CD94 Protein, CF

Recombinant Human CD94 Protein Bioactivity

Recombinant Human CD94 Protein Bioactivity

When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED50 of 0.1-0.6 µg/mL.

Formulation, Preparation, and Storage

9270-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into
disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References

  1. Chester, C. et al. (2015) Front. Immunol. 6:601.
  2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
  3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
  4. Yu, J. et al. (2010) Blood 115:274.
  5. Phillips, J.H. et al. (1996) Immunity 5:163.
  6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
  7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
  8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
  9. Braud, V.M. et al. (1998) Nature 391:795.
  10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
  11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.

Alternate Names

CD94, KLRD1

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Gene Symbol

KLRD1

UniProt

Q13241

Additional CD94 Products

Product Documents for Recombinant Human CD94 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD94 Protein, CF

For research use only

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