Recombinant Human CD94 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse NKG2A/CD159a Fc Chimera (Catalog # 9198-NK) is coated at 0.5 µg/mL, 100 μL/well, recombinant human CD94 binds with a typical ED50 of 0.1-0.6 μg/mL
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Lys32-Ile179, with an N-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Lys32
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    21-38 kDa, reducing conditions
9270-CD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 μg/mL, Recombinant Human CD94 (Catalog #
9270-CD) binds with an ED50 of
0.1-0.6 μg/mL.
Background: CD94
CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into
disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).
  • References:
    1. Chester, C. et al. (2015) Front. Immunol. 6:601.
    2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
    3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
    4. Yu, J. et al. (2010) Blood 115:274.
    5. Phillips, J.H. et al. (1996) Immunity 5:163.
    6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
    7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
    8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
    9. Braud, V.M. et al. (1998) Nature 391:795.
    10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
    11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
  • Entrez Gene IDs:
    3824 (Human)
  • Alternate Names:
    CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor
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