Recombinant Mouse RNF43 Protein Fc Chimera, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Mouse RNF43 Protein Fc Chimera, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated at 0.25 μg/mL, Recombinant Mouse RNF43 Fc Chimera binds with an ED50 = 0.8-4.8 ng/mL.
Mouse myeloma cell line, NS0-derived mouse RNF43 protein
Mouse RNF43
Accession # Q5NCP0
Accession #
N-terminal Sequence
Ala34 & Val36
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa
53-63 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: RNF43

RING finger protein 43 (RNF43) is a 90 kDa member of the ZNRF3 family of ubiquitin ligase proteins (1, 2). Mouse RNF43 is synthesized as a 784 amino acid (aa) protein that contains a putative 23 aa signal sequence, a 174 aa extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain with an atypical RING-type zinc finger (1). RNF43 is expressed in stem cells at the bottom of colon crypts, where it limits the ability of Wnts to induce proliferation (3). RNF43 and ZNRF3, another transmembrane E3 ubiquitin ligase, ubiquitinate and promote the turnover of Frizzled Wnt receptors to antagonize Wnt signaling (3, 4). RNF43 has been shown to suppress both canonical and non-canonical Wnt signaling pathways by distinct mechanisms (5). RNF43/ZNRF3-mediated turnover of Frizzled receptors is inhibited by R-Spondin (4). Dishevelled, a positive regulator of Wnt signaling, interacts with RNF43/ZNRF3 to mediate turnover of Frizzled receptors (6). RNF43 may promote cell survival by binding to NEDL1 and by suppressing the transcriptional activity of p53 (7, 8). RNF43 has been shown both to inhibit and promote cancer. Deletion of RNF43, as well as mutations found in colorectal and other cancers, allows hypersensitivity to Wnts and promotes adenoma formation (3, 9). Furthermore, RNF43 down-regulation in gliomas is associated with poor prognosis (10). However, RNF43 is frequently over-expressed in cancers, correlating with growth-promoting activity and colorectal and hepatocellular cancer pathogenesis (1, 7, 11).

  1. Yagyu, R. et al. (2004) Int. J. Oncol. 25:1343.
  2. de Lau, W. et al. (2014) Genes Dev. 28:305.
  3. Koo, B.K. et al. (2012) Nature 488:665.
  4. Hao, H.X. et al. (2012) Nature 485:195.
  5. Tsukiyama, T. et al. (2015) Mol. Cell. Biol. 35:2007.
  6. Jiang, X. et al. (2015) Mol. Cell 58:522.
  7. Xing, C. et al. (2013) Mol. Cancer Ther. 12:94.
  8. Shinada, K. et al. (2011) Biochem. Biophys. Res. Commun. 404:143.
  9. Giannakis, M. et al. (2014) Nat. Genet. 46:1264.
  10. Xi, S. et al. (2015) Int. J. Clin. Exp. Pathol. 8:490.
  11. Uchida, N. et al. (2004) Clin. Cancer Res. 10:8577.
Long Name
Ring Finger Protein 43
Entrez Gene IDs
54894 (Human); 207742 (Mouse); 303412 (Rat)
Alternate Names
DKFZp781H02126; DKFZp781H0392; FLJ20315; FLJ77466; FLJ99338; MGC125630; ring finger protein 43; RNF124; RNF43; URCC


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse RNF43 Protein Fc Chimera, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse RNF43 Protein Fc Chimera, CF and earn rewards!

Have you used Recombinant Mouse RNF43 Protein Fc Chimera, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review