Recombinant Mouse R-Spondin 3 Protein

Carrier Free

Catalog # Availability Size / Price Qty
4120-RS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
4120-RS-025
Product Details
Citations (10)
FAQs
Reviews

Recombinant Mouse R-Spondin 3 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 3 protein
Met1-His277
Accession #
N-terminal Sequence
Analysis
Met33
Predicted Molecular Mass
28.9 kDa
SDS-PAGE
37-43 kDa, reducing conditions

Product Datasheets

4120-RS (with carrier)

4120-RS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4120-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4120-RS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 3

R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% aa identity with the other three R‑Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1-4). Like other R‑spondins, R‑Spondin 3 contains two adjacent cysteine-rich furin‑like domains (amino acids (aa) 35-135) with one potential N‑glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21-209, mouse R‑Spondin 3 shares 93%, 97%, 96%, 95% and 91% aa identity with human, rat, equine, bovine and canine R‑Spondin 3, respectively. Potential isoforms of 217, 224 and 252 aa are divergent or truncated at the C terminus; the 252 aa form also lacks aa 4‑33 at the N‑terminus (5). Mouse R‑Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta-specific transcription factor, Gcm1. In the mouse embryo, R‑Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (8, 9). Reports differ on whether R‑Spondins bind LRP-6 directly (8-10). R‑Spondin 3 has also been identified as an oncogene (11).

References
  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  5. Entrez Accession # BAB28811, BAC36296 and EDL04842.
  6. Kazanskaya, O. et al. (2008) Development 135:3655.
  7. Aoki, M. et al. (2007) Dev. Biol. 301:218.
  8. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  9. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  10. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  11. Theodorou, V. et al. (2007) Nat. Genet. 6:759.
Long Name
Roof Plate-specific Spondin 3
Entrez Gene IDs
84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)
Alternate Names
Cristin 1; CRISTIN1; FLJ14440; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; Roof plate-specific spondin-3; RSPO3; R-spondin 3 homolog (Xenopus laevis); RSpondin 3; R-Spondin 3; R-spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2; THSD2

Citations for Recombinant Mouse R-Spondin 3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
    Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
    PLoS ONE, 2020;15(3):e0229445.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Molecular determinants of WNT9b responsiveness in nephron progenitor cells
    Authors: KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer
    PLoS ONE, 2019;14(4):e0215139.
  3. Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
    Authors: R Ogasawara, D Hashimoto, S Kimura, E Hayase, T Ara, S Takahashi, H Ohigashi, K Yoshioka, T Tateno, E Yokoyama, K Ebata, T Kondo, J Sugita, M Onozawa, T Iwanaga, T Teshima
    Sci Rep, 2018;8(1):10719.
    Applications: Western Blot
  4. PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo
    Authors: G Greicius, Z Kabiri, K Sigmundsso, C Liang, R Bunte, MK Singh, DM Virshup
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. R-spondin3-LGR4 signaling protects hepatocytes against DMOG-induced hypoxia/reoxygenation injury through activating ?-catenin
    Authors: S Liu, Y Yin, R Yu, Y Li, W Zhang
    Biochem. Biophys. Res. Commun., 2018;499(1):59-65.
    Species: Mouse
    Sample Types: AML12 cells
    Applications: Bioassay
  6. The Stem Cell Marker Lgr5 Defines a Subset of Post-Mitotic Neurons in the Olfactory Bulb
    Authors: Y Yu, AH Moberly, JP Bhattarai, C Duan, Q Zheng, F Li, H Huang, W Olson, W Luo, T Wen, H Yu, M Ma
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016;7(39):63767-63778.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3.
    Authors: Cambier L, Plate M, Sucov H, Pashmforoush M
    Development, 2014;141(15):2959-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
    Authors: Li, Ji-Yao, Chai, Biaoxin, Zhang, Weizhen, Fritze, Danielle, Zhang, Chao, Mulholland, Michael
    Endocrinology, 2014;155(2):429-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Lgr4 is a key regulator of prostate development and prostate stem cell differentiation.
    Authors: Luo W, Rodriguez M, Valdez J, Zhu X, Tan K, Li D, Siwko S, Xin L, Liu M
    Stem Cells, 2013;31(11):2492-505.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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