Recombinant Mouse Semaphorin 3A Fc Chimera Protein

Carrier Free

Catalog # Availability Size / Price Qty
5926-S3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5926-S3-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (16)
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Reviews (1)

Recombinant Mouse Semaphorin 3A Fc Chimera Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Mouse Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta ‑NGF (Catalog # 256-GF).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Semaphorin 3A protein
Mouse Semaphorin 3A
(Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala)
Accession # O08665
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asn21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
110.1 kDa (monomer)
SDS-PAGE
115-120 kDa, reducing conditions

Product Datasheets

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5926-S3 (with carrier)

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5926-S3/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5926-S3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5926-S3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 3A

Semaphorin 3A (Sema3A; previously sem D, sema III or collapsin) is one of six Class 3 secreted semaphorins which share ~40-50% amino acid (aa) identity
(1-3). Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development (2, 3). The 772 aa mouse Sema3C contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a PSI domain, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (3, 4). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (5, 6). The 95 kDa mature mouse Sema3A shares at least 95% aa identity with human, rat, equine and canine Sema3A, and 90% and 86% aa identity with chick and zebrafish Sema3A, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (3). Sema3A signaling is transduced by plexin A1-4, indirectly via neuropilin-1 (3). Sema3A activity is mediated by small GTPases that influence actin rearrangement and integrin activity (7-9). It is important in developmental organization of central and peripheral nerves, including those in heart, lung, kidneys, bones, teeth, and visual and olfactory systems (1, 2, 10, 11). Gradients of Sema3A repel axons, but attract dendrites (11, 12). Sema3A affect vasculogenesis by inhibiting integrin function and, with Sema3F, promoting apoptosis of endothelial cells (3, 9, 12). It is thought to suppress cancer-related angiogenesis (3). In the immune system, Sema3A influences T cell proliferation, migration, response to activation, and interactions with dendritic cells (7, 13). It negatively regulates platelet activation (14). Expression of Sema3A in relevant parts of the nervous system may be increased in Alzheimer’s disease, multiple sclerosis, ischemia and schizophrenia (2).

References
  1. Puschel, A.W. et al. (1995) Neuron 14:941.
  2. Roth, L. et al. (2009) Cell. Mol. Life Sci. 66:649.
  3. Neufeld, G and O. Kessler (2008) Nat. Rev. Cancer 8:632.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  6. Klosterman, A. et al. (1998) J. Biol. Sci. 273:7326.
  7. Lepelletier, Y. et al. (2006) Eur. J. Immunol. 36:1782.
  8. Schlomann, U. et al. (2009) J. Cell Sci. 122:2034.
  9. Serini, G. et al. (2003) Nature 424:391.
  10. Ieda, M. et al. (2007) Nat. Med. 13:604.
  11. Chen, G. et al. (2008) Nat. Neurosci. 11:36.
  12. Guttmann-Raviv, N. et al. (2007) J. Biol Chem. 282:26294.
  13. Lepelletier, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5545.
  14. Kashiwagi, H. et al. (2005) Blood 106:913.
Entrez Gene IDs
10371 (Human); 20346 (Mouse); 29751 (Rat); 100620398 (Porcine); 102126862 (Cynomolgus Monkey)
Alternate Names
(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD

Citations for Recombinant Mouse Semaphorin 3A Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Semaphorin 3A regulates alveolar bone remodeling on orthodontic tooth movement
    Authors: H Kamei, T Ishii, Y Nishii
    Oncogene, 2022;12(1):9243.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Semaphorin3A-Inhibitor Ameliorates Doxorubicin-Induced Podocyte Injury
    Authors: Y Sang, K Tsuji, A Inoue-Tori, K Fukushima, S Kitamura, J Wada
    Int J Mol Sci, 2020;21(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. SARS-CoV-2 Spike protein co-opts VEGF-A/Neuropilin-1 receptor signaling to induce analgesia
    Authors: A Moutal, LF Martin, L Boinon, K Gomez, D Ran, Y Zhou, HJ Stratton, S Cai, S Luo, KB Gonzalez, S Perez-Mill, A Patwardhan, MM Ibrahim, R Khanna
    bioRxiv : the preprint server for biology, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. In vivo topology converts competition for cell-matrix adhesion into directional migration
    Authors: F Bajanca, N Gouignard, C Colle, M Parsons, R Mayor, E Theveneau
    Nat Commun, 2019;10(1):1518.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Adenosine Areceptor (A2AR) stimulation modulates expression of semaphorins 4D and 3A, regulators of bone homeostasis
    Authors: A Mediero, T Wilder, L Shah, BN Cronstein
    FASEB J., 2018;0(0):fj201700217R.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Differentiation
  7. Sema3A promotes the resolution of cardiac inflammation after myocardial infarction
    Authors: M Rienks, P Carai, N Bitsch, M Schellings, M Vanhaverbe, J Verjans, I Cuijpers, S Heymans, A Papageorgi
    Basic Res. Cardiol., 2017;112(4):42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Efferocytosis Assay
  8. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage.
    Authors: Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli
    Sci Rep, 2015;5(0):7890.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: In vivo
  9. Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5.
    Authors: Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, Asada A, Kamada S, Ohshima T, Hisanaga S
    J Biol Chem, 2014;289(28):19627-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Nogo receptor homolog NgR2 expressed in sensory DRG neurons controls epidermal innervation by interaction with Versican.
    Authors: Baumer B, Kurz A, Borrie S, Sickinger S, Dours-Zimmermann M, Zimmermann D, Bandtlow C
    J Neurosci, 2014;34(5):1633-46.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The serine/threonine kinase Ndr2 controls integrin trafficking and integrin-dependent neurite growth.
    Authors: Rehberg K, Kliche S, Madencioglu D, Thiere M, Muller B, Meineke B, Freund C, Budinger E, Stork O
    J Neurosci, 2014;34(15):5342-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Activity-dependent regulation of dendritic complexity by semaphorin 3A through Farp1.
    Authors: Cheadle L, Biederer T
    J Neurosci, 2014;34(23):7999-8009.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
    Authors: Delgoffe, Greg M, Woo, Seng-Ryo, Turnis, Meghan E, Gravano, David M, Guy, Cliff, Overacre, Abigail, Bettini, Matthew, Vogel, Peter, Finkelstein, David, Bonnevier, Jody, Workman, Creg J, Vignali, Dario A
    Nature, 2013;501(7466):252-6.
    Species: N/A
    Sample Types: N/A
    Applications: Binding Assay
  14. Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
    Authors: Chakraborty G, Kumar S, Mishra R
    PLoS ONE, 2012;7(3):e33633.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Osteoprotection by semaphorin 3A.
    Authors: Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H
    Nature, 2012;485(7396):69-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse.
    Authors: Naska S, Lin DC, Miller FD
    Mol. Cell. Neurosci., 2010;45(2):108-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Semaphorin 3A Fc Chimera Protein
By Nan Gao on 10/27/2017
Application: In vivo study