Recombinant Human beta-NGF Protein

(22 citations)
(2 Reviews)
  
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2‑2 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ser122-Ala241
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser122
  • Predicted Molecular Mass
    13.5 kDa (monomer)
  • SDS-PAGE
    13 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
256-GF
 
256-GF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human beta -NGF (Catalog # 256‑GF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2‑2 ng/mL.
1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and  gamma. Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

  • Long Name:
    beta Nerve Growth Factor
  • Entrez Gene IDs:
    4803 (Human); 18049 (Mouse); 310738 (Rat)
  • Alternate Names:
    Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury
    Authors: S Delbary-Go, S Lee, M Baroni, I Lamarche, M Arnone, B Canolle, A Lin, J Sacramento, EA Salegio, MN Castel, N Delesque-T, A Alam, P Laboudie, B Ferzaz, P Savi, JM Herbert, GT Manley, AR Ferguson, JC Bresnahan, F Bono, MS Beattie
    Brain, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models
    Sci Rep, 2016;6(0):33427.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo
    Sci Rep, 2016;6(0):38644.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  4. Nerve Growth Factor Attenuates Apoptosis and Inflammation in the Diabetic Cornea
    Invest. Ophthalmol. Vis. Sci, 2016;57(15):6767-6775.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Hierarchical CRMP2 posttranslational modifications control NaV1.7 function
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Effects of Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression on Dendritic Outgrowth and Demyelination after Spinal Cord Injury.
    Authors: Park K, Lin C, Li K, Lee Y
    PLoS ONE, 2015;10(9):e0138301.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy.
    Authors: Schwab, Andrew J, Ebert, Allison
    PLoS ONE, 2014;9(7):e103112.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
    Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
    Stem Cells Transl Med, 2014;3(3):334-45.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  9. 2, 3, 7, 8-Tetrachlorodibenzo-P-dioxin (TCDD) induces premature senescence in human and rodent neuronal cells via ROS-dependent mechanisms.
    Authors: Wan C, Liu J, Nie X, Zhao J, Zhou S, Duan Z, Tang C, Liang L, Xu G
    PLoS ONE, 2014;9(2):e89811.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  10. Neuropeptides released from trigeminal neurons promote the stratification of human corneal epithelial cells.
    Authors: Ko J, Mizuno Y, Ohki C, Chikama T, Sonoda K, Kiuchi Y
    Invest Ophthalmol Vis Sci, 2014;55(1):125-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation.
    Authors: Bowers P, Neben T, Tomlinson G, Dalton J, Altobell L, Zhang X, Macomber J, Wu B, Toobian R, McConnell A, Verdino P, Chau B, Horlick R, King D
    J Biol Chem, 2013;288(11):7688-96.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  12. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  13. EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells.
    Authors: Delcroix GJ, Curtis KM, Schiller PC, Montero-Menei CN
    Differentiation, 2010;80(4):213-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
    Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
    Mol. Ther., 2010;18(12):2164-72.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  15. Down-regulation of the met receptor tyrosine kinase by presenilin-dependent regulated intramembrane proteolysis.
    Authors: Foveau B, Ancot F, Leroy C, Petrelli A, Reiss K, Vingtdeux V, Giordano S, Fafeur V, Tulasne D
    Mol. Biol. Cell, 2009;20(9):2495-507.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  16. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008;151(3):476-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat.
    Authors: Paredes D, Granholm AC, Bickford PC
    Brain Res., 2007;1141(0):56-64.
  18. RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction.
    Authors: Gallo G
    J. Cell. Sci., 2006;119(0):3413-23.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  19. Elicitation of immunity to HIV type 1 Gag is determined by Gag structure.
    Authors: Young KR, Ross TM
    AIDS Res. Hum. Retroviruses, 2006;22(1):99-108.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  20. Nerve growth factor expression by PLG-mediated lipofection.
    Authors: Whittlesey KJ, Shea LD
    Biomaterials, 2006;27(11):2477-86.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  21. Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide.
    Authors: Adams DN, Kao EY, Hypolite CL, Distefano MD, Hu WS, Letourneau PC
    J. Neurobiol., 2005;62(1):134-47.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  22. Nerve growth factor overexpression and autocrine loop in breast cancer cells.
    Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
    Oncogene, 2003;22(36):5592-601.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 22 Citations
Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 2 applications: Stem/Immune cell maintenance or differentiation , In my cell culture media to grow microglia cells.
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  Excellent
 Stem/Immune cell maintenance or differentiation Anonymous 05/20/2017
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ApplicationStem/Immune cell maintenance or differentiation
In my cell culture media to grow microglia cells Recombinant Human beta-NGF Protein 256-GF
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Excellent
 In my cell culture media to grow microglia cells Anonymous 04/21/2017
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In my cell culture media to grow microglia cells Recombinant Human beta-NGF Protein 256-GF
In my cell culture media to grow microglia cells: Recombinant Human beta-NGF Protein [256-GF]

Summary

ApplicationIn my cell culture media to grow microglia cells
Reason for RatingI found R&D growth factors quality much more superior than other sources.

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