Recombinant Viral CCI Fc Chimera Protein, CF

R&D Systems | Catalog # 696-CC

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Viral CCI Fc Chimera Protein (696-CC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P19063

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived viral CCI protein
Viral CCI
(Met17-Val258)&
(Pro18-Val258)
Accession # P19063		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met17 & Pro18

Predicted Molecular Mass

57 kDa (monomer)

Activity

Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR2A.
The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL of recombinant mouse JE.

Scientific Data Images for Recombinant Viral CCI Fc Chimera Protein, CF

Recombinant Viral CCI Fc Chimera Protein Bioactivity

Recombinant Viral CCI Fc Chimera Protein Bioactivity

Recombinant Viral CCI Fc Chimera (Catalog # 696-CC) inhibits Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR2A. The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL2/JE/MCP-1.

Formulation, Preparation, and Storage

696-CC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCI

The family of T1/35 kDa proteins are encoded by the '35K' virulence gene of many poxviruses and are secreted from virus-infected cells. These proteins bind
CC-chemokines with high affinity and are termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of sequence identity and eight conserved cysteine residues (1). The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) protein with a putative 17 aa signal peptide (2). Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. vCCI binds with high affinity to many human, mouse, and rat CC-chemokines (1, 3-5). It binds to the same surfaces of these chemokines that are involved in chemokine receptor interactions (4-7). Some CC-chemokines utilize common binding surfaces on vCCI, while others interact with distinct sites on vCCI (8). vCCI inhibits CC-chemokine induced monocyte chemotaxis in vitro but does not block cellular effects induced by CXCL5/ENA-78, CXCL8/IL-8, or CXCL10/IP-10 (3-5). In vivo, vCCI restricts the infiltration of  immune cells into sites of inflammation (3, 9, 10) and also the CC-chemokine induced migration of arterial vascular smooth muscle cells (11).

References

  1. Graham, K.A. et al. (1997) Virology 229:12.
  2. Patel, A.H. et al. (1990) J. Gen. Virol. 71:2013.
  3. Buatois, V. et al. (2010) J. Immunol. 185:2544.
  4. Smith, C.A. et al. (1997) Virology 236:316.
  5. Lalani, A.S. et al. (1998) Virology 250:173.
  6. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
  7. Seet, B.T. et al. (2001) Proc. Natl. Acad. Sci. 98:9008.
  8. Burns, J.M. et al. (2002) J. Biol. Chem. 277:2785.
  9. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
  10. Reading, P.C. et al. (2003) J. Immunol. 170:1435.
  11. Spinetti, G. et al. (2004) Arterioscler. Throm. Vasc. Biol. 24:1397.

Long Name

CC Chemokine Inhibitor

UniProt

P19063 (Viral)

Additional CCI Products

Product Documents for Recombinant Viral CCI Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Viral CCI Fc Chimera Protein, CF

For research use only

Related Research Areas

Infectious Diseases

Citations for Recombinant Viral CCI Fc Chimera Protein, CF

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