Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein, CF

R&D Systems | Catalog # 9450-ZK

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein (9450-ZK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AHZ13508
View all Zika Virus (H/PF/2013) NS1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral Zika Virus (H/PF/2013) NS1 protein
Val796 & Ser799-Ser1148, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val796 & Ser799

Predicted Molecular Mass

41 kDa

SDS-PAGE

42-61 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9450-ZK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Zika Virus (H/PF/2013) NS1

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host.  ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

References

  1. Song, H. et al. (2016) Nat. Struct. and Mol. Biol. 23:456.
  2. Brown, WC. et al. (2016) Nat. Struct. and Mol. Biol. 23: 865.
  3. Baronti, C. et al. (2014) Genome Announc. 2: e00500

Long Name

Zika Virus Polyprotein

UniProt

AHZ13508

Additional Zika Virus (H/PF/2013) NS1 Products

Product Documents for Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein, CF

For research use only

Related Research Areas

Infectious Diseases

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