Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RFC4 Primary Antibodies »

Product Specifications

Immunogen

A 23 amino acid synthetic peptide, which represented a portion of the human Replication Factor C 4, 37 kDa subunit encoded within exon 2 (locusLink ID 5984).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFC4 Antibody - BSA Free

Western Blot: RFC4 Antibody [NB100-403]

Western Blot: RFC4 Antibody [NB100-403]

Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.

Applications for RFC4 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
NB 100-403 may be used for Western Blot and immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFC4

Human replication factor C (RFC) is a hetero-pentameric protein. RFC p37 (RFC4) encodes subunit 4 of the RFC complex, which functions in DNA checkpoint control. It plays a role in mitotic cohesion and condensation.

Alternate Names

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, MGC27291, replication factor C (activator 1) 4, 37kDa, Replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37replication factor C (activator 1) 4 (37kD)

Entrez Gene IDs

5984 (Human)

Gene Symbol

RFC4

Additional RFC4 Products

Product Documents for RFC4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RFC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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