RFP Antibody

Novus Biologicals | Catalog # NBP3-50134

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RFP Primary Antibodies »

Product Specifications

Immunogen

Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Specificity

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.

Applications for RFP Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been tested by and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27kDa in Western Blot in the appropriate cell lysate or extract.

Suggested applications: EM; FC; IF; IHC; Multiplex

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFP

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Alternate Names

DSRED, GFP-like chromoprotein, Red Fluorescent Protein, RFP

Additional RFP Products

Product Documents for RFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RFP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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