Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RFXANK Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human RFXANK fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RFXANK Antibody - BSA Free

Western Blot: RFXANK Antibody [NBP2-97640]

Western Blot: RFXANK Antibody [NBP2-97640]

Western Blot: RFXANK Antibody [NBP2-97640] - Anti-RFXANK rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 33 kDa
Immunoprecipitation: RFXANK Antibody [NBP2-97640]

Immunoprecipitation: RFXANK Antibody [NBP2-97640]

Immunoprecipitation: RFXANK Antibody [NBP2-97640] - RFXANK was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate 4 ul anti-RFXANK rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFXANK rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 34 kDa

Applications for RFXANK Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFXANK

RFX-B is the gene responsible for the bare lymphocyte syndrome (BLS) which is characterized by a lack of MHC class II transcription. Subsequently, there is a lack of B and T cell responses to foreign antigens. RFX-B is an MHC class II transcription factor that is defective in BLS.

Alternate Names

ANKRA1Regulatory factor X subunit B, ankyrin repeat-containing regulatory factor X-associated protein, BLS, DNA-binding protein RFXANK, F14150_1, MGC138628, regulatory factor X-associated ankyrin-containing proteinAnkyrin repeat family A protein 1, RFXB, RFX-BRFX-Bdelta4

Gene Symbol

RFXANK

Additional RFXANK Products

Product Documents for RFXANK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RFXANK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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