RNF2 Antibody (9Q2W2)
Novus Biologicals | Catalog # NBP3-16222
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9Q2W2 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RNF2 (Q99496). LRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RNF2 Antibody (9Q2W2) (NBP3-16222) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RNF2 Antibody (9Q2W2)
Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222]
Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222] - Western blot analysis of extracts of various cell lines, using RNF2 Rabbit mAb (NBP3-16222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunoprecipitation: RNF2 Antibody (9Q2W2) [NBP3-16222]
Immunoprecipitation: RNF2 Antibody (9Q2W2) [NBP3-16222] - Immunoprecipitation analysis of 300ug extracts of U-937 cells using 3ug RNF2 antibody (NBP3-16222). Western blot was performed from the immunoprecipitate using RNF2 antibody (NBP3-16222) at a dilition of 1:500.Applications for RNF2 Antibody (9Q2W2)
Application
Recommended Usage
Immunoprecipitation
1:100 - 1:500
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RNF2
Long Name
Ring Finger Protein 2
Alternate Names
DING, HIPI3, RING1B, RING2
Gene Symbol
RNF2
Additional RNF2 Products
Product Documents for RNF2 Antibody (9Q2W2)
Product Specific Notices for RNF2 Antibody (9Q2W2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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