RPA70 Antibody (5R7S3)

Novus Biologicals | Catalog # NBP3-16174

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5R7S3 expressed in HEK293
View all RPA70 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70 (P27694). MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPA70 Antibody (5R7S3)

Western Blot: RPA70 Antibody (5R7S3) [NBP3-16174]

Western Blot: RPA70 Antibody (5R7S3) [NBP3-16174]

Western Blot: RPA70 Antibody (5R7S3) [NBP3-16174] - Western blot analysis of extracts of various cell lines, using RPA70 Rabbit mAb (NBP3-16174) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174]

Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174]

Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RPA70 antibody (NBP3-16174). Western blot was performed from the immunoprecipitate using RPA70 antibody (NBP3-16174) at a dilition of 1:1000.
RPA70 Antibody (5R7S3)

Chromatin Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174] -

Chromatin Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RPA70 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
RPA70 Antibody (5R7S3)

Chromatin Immunoprecipitation: RPA70 Antibody (5R7S3) [RPA70] -

Chromatin Immunoprecipitation: RPA70 Antibody (5R7S3) [RPA70] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RPA70 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for RPA70 Antibody (5R7S3)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500-1:1000

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPA70

RPA (Replication protein A) is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is a single strand binding protein that is involved in replication, homologous recombination and nucleotide excision and repair. It colocalises with RAD51 on synapsed axes in meiosis, facilitating RAD51 function. Replication protein A acts as an auxiliary protein for DNA polymerases alpha and delta.

Alternate Names

HSSB, MST075, MSTP075, REPA1RF-A protein 1, Replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1 (70kD), replication protein A1, 70kDa, RF-A, RP-A, RPA70RP-A p70, Single-stranded DNA-binding protein

Gene Symbol

RPA1

Additional RPA70 Products

Product Documents for RPA70 Antibody (5R7S3)

Certificate of Analysis

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Product Specific Notices for RPA70 Antibody (5R7S3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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