Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-40093

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.29 ng/mL (example only; lot dependent)

Assay Range

0.781 - 50 ng/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40093]

ELISA: Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40093] - Standard Curve Reference

Kit Contents for Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SAT1

SAT1--also known as Spermidine/spermine-N1-acetyltransferase (SSAT1)--is a short-lived polyamine catabolic enzyme which catalyzes the acetylation of spermidine and spermine. SAT1 is a highly regulated enzyme which allows a fine attenuation of the intracellular concentration of polyamines. Induction of SAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase.

Alternate Names

DC21, diamine acetyltransferase 1, diamine N-acetyltransferase 1, EC 2.3.1.57, KFSD, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase, SAT, Spermidine/spermine N(1)-acetyltransferase 1, spermidine/spermine N1-acetyltransferase, spermidine/spermine N1-acetyltransferase 1, spermidine/spermine N1-acetyltransferase alpha, SSAT-1, SSATKFSDX

Gene Symbol

SAT1

Additional SAT1 Products

Product Documents for Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human SAT1 - Ready-To-Use ELISA Kit (Colorimetric)

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  • Q: I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?

    A:

    I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in The Journal of Biological Chemistry 2009;284(12):8174-8184 (https://www.jbc.org/article/S0021-9258(20)32529-1/fulltext).

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