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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
between 75 and 125
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SF3A3 Antibody
Western Blot:[NBP3-30293]-
Western Blot:[NBP3-30293]-Detection of human and mouse SF3A3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 3 µg/mg lysate. SF3A3 was more efficiently immunoprecipitated by rabbit anti-SF3A3 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.Applications for SF3A3 Antibody
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-buffered Saline 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SF3A3
Alternate Names
PRP9, PRPF9, SAP 61, SAP61splicing factor 3a, subunit 3, 60kD, SF3a60pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, Spliceosome-associated protein 61, splicing factor 3A subunit 3, splicing factor 3a, subunit 3, 60kDa
Gene Symbol
SF3A3
Additional SF3A3 Products
Product Documents for SF3A3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SF3A3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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