Recombinant Human SPARC Protein

Novus Biologicals | Catalog # NBP2-61362

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity
View all SPARC Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 286 amino acids corresponding to SPARC Source: E. coli

Uniprot ID: P09486

Amino Acid Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of SPARC as determined by LAL method.

Predicted Molecular Mass

32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

SPARC protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SPARC Protein

Recombinant Human SPARC Protein

SDS-Page: Recombinant Human SPARC Protein [NBP2-61362]

SDS-Page: Recombinant Human SPARC Protein [NBP2-61362]

Formulation, Preparation, and Storage

NBP2-61362
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

Additional SPARC Products

Product Documents for Recombinant Human SPARC Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human SPARC Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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