tdTomato Antibody (OTI2H2)

Western Blot: tdTomato Antibody (OTI2H2) [NBP2-78136] -

Construction of tdTomato-fused synaptotagmin-1 (Syt1-tdTomato) expression vector and transgenic fluorescent mice.(A) Schematic diagram of expression vector for generating Syt1-tdTomato transgenic mice. (B) The expression vector was digested by restriction enzymes for confirmation purposes. The size of enzyme digestion band (kb): (left) AvaI: 4.7/1.7/0.7/0.5; (middle) AflII/NcoI: 4.0/1.3/1.1/0.7/0.4/0.2; (right) NotI: 7.6. (C) Immunoblot analysis of protein in transgenic and wild-type mice brain. Equivalent amounts of protein were probed with antibodies to tdTomato and vasolin-containing protein (VCP, used as a loading control). (D) Immunoblot analysis of the expression of the transgenic protein in different tissues of Syt1-tdTomato transgenic mice. Equivalent amounts of protein were examined by immunoblotting with antibodies to tdTomato, SYT1, and VCP. Asterisk shows the band of SYT1-tdTomato and the hash shows the band of native SYT1.Figure 1—source data 1.The original gel of panels B, C and D.The original gel of panels B, C and D. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/81884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: tdTomato Antibody (OTI2H2) [NBP2-78136] -

Construction of tdTomato-fused synaptotagmin-1 (Syt1-tdTomato) expression vector and transgenic fluorescent mice.(A) Schematic diagram of expression vector for generating Syt1-tdTomato transgenic mice. (B) The expression vector was digested by restriction enzymes for confirmation purposes. The size of enzyme digestion band (kb): (left) AvaI: 4.7/1.7/0.7/0.5; (middle) AflII/NcoI: 4.0/1.3/1.1/0.7/0.4/0.2; (right) NotI: 7.6. (C) Immunoblot analysis of protein in transgenic and wild-type mice brain. Equivalent amounts of protein were probed with antibodies to tdTomato and vasolin-containing protein (VCP, used as a loading control). (D) Immunoblot analysis of the expression of the transgenic protein in different tissues of Syt1-tdTomato transgenic mice. Equivalent amounts of protein were examined by immunoblotting with antibodies to tdTomato, SYT1, and VCP. Asterisk shows the band of SYT1-tdTomato and the hash shows the band of native SYT1.Figure 1—source data 1.The original gel of panels B, C and D.The original gel of panels B, C and D. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/81884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.