TFIIE beta Antibody

Novus Biologicals | Catalog # NBP2-20610

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TFIIE beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TFIIE beta. The exact sequence is proprietary.

Reactivity Notes

Bovine (87%), Mouse (86%), Rat (86%), Pig (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TFIIE beta Antibody

Western Blot: TFIIE beta Antibody [NBP2-20610]

Western Blot: TFIIE beta Antibody [NBP2-20610]

Western Blot: TFIIE beta Antibody [NBP2-20610] - Sample (30 ug of whole cell lysate) A: Jurkat nucleus 10% SDS PAGE gel, diluted at 1:5000.
Chromatin Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
TFIIE beta Antibody

Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] -

TFIIE beta antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 ug whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 ug of TFIIE beta antibody (NBP2-20610) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody (NBP2-20610) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Applications for TFIIE beta Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFIIE beta

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Alternate Names

FE, General transcription factor IIE subunit 2, general transcription factor IIE, polypeptide 2 (beta subunit, 34kD), general transcription factor IIE, polypeptide 2, beta 34kDa, TF2E2TFIIE-beta, TFIIE-B, transcription initiation factor IIE subunit beta

Gene Symbol

GTF2E2

UniProt

P29084 (Human)

Additional TFIIE beta Products

Product Documents for TFIIE beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TFIIE beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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