Thioredoxins (Trxs) are a group of small ubiquitous proteins that are key regulators of cellular redox balance. The mammalian Trx family includes the secreted and cellular protein (Trx-1), the mitochondria-specific Trx-2, the Trx-like cytosolic protein p32TrxL, a truncated version of Trx-1 known as Trx80, and the thioredoxin-related protein, TRP-14. Trx-1 is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Trx-1 acts as a growth factor an, antioxidant, a cofactor that provides reducing equivalents, and a transcriptional regulator. Expression of Trx-1 is increased under various stress conditions such as hypoxia and viral and bacterial infections. It also serves as a biomarker for rheumatoid arthritis.
Human Thioredoxin-1 ELISA Kit (Chemiluminescence)
Novus Biologicals | Catalog # NBP2-68158
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
37.50 pg/mL
Assay Range
62.50-4000 pg/mL
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Product Specifications
Assay Type
Sandwich-CLIA
Kit Type
ELISA Kit (Chemiluminescence)
Species
Human
Specificity
This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 7.82%
Inter-Assay Precision (Precision between assays) CV% 9.32%
Recovery for Human Thioredoxin-1 ELISA Kit (Chemiluminescence)
Recovery
94-116%
Linearity
Scientific Data Images for Human Thioredoxin-1 ELISA Kit (Chemiluminescence)
ELISA: Human Thioredoxin-1 ELISA Kit (Chemiluminescence) [NBP2-68158] -
ELISA: Human Thioredoxin-1 ELISA Kit (Chemiluminescence) [NBP2-68158] - Standard Curve ReferenceKit Contents for Human Thioredoxin-1 ELISA Kit (Chemiluminescence)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Thioredoxin-1
Alternate Names
Thioredoxin1, Trx1, TXN, TXN1
Gene Symbol
TXN
Additional Thioredoxin-1 Products
Product Documents for Human Thioredoxin-1 ELISA Kit (Chemiluminescence)
Product Specific Notices for Human Thioredoxin-1 ELISA Kit (Chemiluminescence)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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