Recombinant Human UBE2K/E2-25K His (N-Term) Protein

Name: Recombinant Human UBE2K/E2-25K His (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-35096
Price: 2252 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-35096

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession Number

P61086

Tag

His (N-Term)

Applications

SDS-PAGE

View all UBE2K/E2-25K Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 200 amino acids corresponding to UBE2K/E2-25K and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

Uniprot ID: P61086

Amino Acid Sequence: MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of UBE2K/E2-25K as determined by LAL method.

Predicted Molecular Mass

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

SDS-PAGE: Recombinant Human UBE2K/E2-25K His (N-Term) Protein [NBP2-35096]

SDS-Page: Human UBE2K/E2-25K His Protein [NBP2-35096]

Formulation, Preparation, and Storage

NBP2-35096

Formulation	A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative	5% Trehalose
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution	Please contact technical support for detailed reconstitution instructions.
Format	Carrier-Free
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: UBE2K/E2-25K

Ubiquitin-conjugating enzyme E2 K (UBE2K) is a protein that in humans is encoded by the UBE2K gene. The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.

Long Name

Ubiquitin-conjugating Enzyme E2

Alternate Names

E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K

Gene Symbol

UBE2K

UniProt

P61086 (Human)

Additional UBE2K/E2-25K Products

Product Documents for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Customer Reviews for Recombinant Human UBE2K/E2-25K His (N-Term) Protein

There are currently no reviews for this product. Be the first to review Recombinant Human UBE2K/E2-25K His (N-Term) Protein and earn rewards!

Have you used Recombinant Human UBE2K/E2-25K His (N-Term) Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image