Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1). GPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UPF2 Antibody - BSA Free
Western Blot: UPF2 Antibody - BSA Free [NBP2-94519] -
Western Blot: UPF2 Antibody - BSA Free [NBP2-94519] - Western blot analysis of various lysates, using UPF2 Rabbit pAb (A7091) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry: UPF2 Antibody - BSA Free [NBP2-94519] -
Immunohistochemistry: UPF2 Antibody - BSA Free [NBP2-94519] - Immunohistochemistry analysis of UPF2 in paraffin-embedded Human kidney tissue using UPF2 Rabbit pAb (A7091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation: UPF2 Antibody - BSA Free [NBP2-94519] -
Immunoprecipitation: UPF2 Antibody - BSA Free [NBP2-94519] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug UPF2 Rabbit pAb (A7091 1:200). Western blot was performed from the immunoprecipitate using UPF2 Rabbit pAb (A7091) at a dilition of 1:1000.Applications for UPF2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UPF2
Alternate Names
DKFZp434D222, hUpf2, KIAA1408MGC138835, Nonsense mRNA reducing factor 2, regulator of nonsense transcripts 2, RENT2MGC138834, smg-3, UPF2 regulator of nonsense transcripts homolog (yeast), Up-frameshift suppressor 2 homolog, yeast Upf2p homolog
Gene Symbol
UPF2
Additional UPF2 Products
Product Documents for UPF2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UPF2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for UPF2 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review UPF2 Antibody - BSA Free and earn rewards!
Have you used UPF2 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...