VAV2 Antibody (Polyclonal) - BSA Free

Novus Biologicals | Catalog # NBP3-30791

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG Clone # Polyclonal

Format

BSA Free
View all VAV2 Primary Antibodies »

Product Specifications

Immunogen

Sheep were immunized with a his tagged fusion protein VAV2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for VAV2 Antibody (Polyclonal) - BSA Free

VAV2 Antibody (Polyclonal)

Western Blot: VAV2 Antibody (Polyclonal) [NBP3-30791] -

Western Blot: VAV2 Antibody (Polyclonal) [NBP3-30791] - Legend: HEK293 cells were transfected with either emply expression vector (pSR␣ ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wrester blot Ab - SHD anti Vav2 antibody 1:10000.

Applications for VAV2 Antibody (Polyclonal) - BSA Free

Application
Recommended Usage

Western Blot

: 1:2500 -1:5000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

Phosphate buffered saline

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAV2

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav and Vav2 are members of the dbl family of guanine nucleotide exchange factors (GEF) for the rho/rac family of GTP binding proteins. Vav is expressed primarily in hematopoietic cells, while vav2 has a wider tissue distribution (2). Vav2 serves as a guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Unlike Vav1, whose expression is restricted to cells of hematopoietic origin, Vav2 is broadly expressed. Recently, Vav2 has been identified as a substrate for the epidermal growth factor (EGF) receptor. EGF receptor phosphorylates Vav2 on all three possible phosphorylation sites, Tyr-142, Tyr-159, and Tyr-172. results suggest that EGF regulates Vav2 activity basically through phosphatidylinositol 3-kinase activation, whereas tyrosine phosphorylation of Vav2 may rather be necessary for mediating protein-protein interactions (3)

Alternate Names

guanine nucleotide exchange factor VAV2, oncogene VAV2, Protein vav-2, vav 2 guanine nucleotide exchange factor, vav 2 oncogene, VAV-2

Gene Symbol

VAV2

Additional VAV2 Products

Product Documents for VAV2 Antibody (Polyclonal) - BSA Free

Certificate of Analysis

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Product Specific Notices for VAV2 Antibody (Polyclonal) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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