Human VEGF 165 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-79681

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, and other biological fluids. (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human VEGF165 in samples. No Significant cross-reactivity or interference between Human VEGF165 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 4.65

Inter-Assay Precision (Precision between assays) %CV 4.79

Recovery for Human VEGF 165 ELISA Kit (Colorimetric)

Recovery

83 - 107%

Linearity

Human VEGF 165 ELISA Kit (Colorimetric)

Scientific Data Images for Human VEGF 165 ELISA Kit (Colorimetric)

Human VEGF 165 ELISA Kit (Colorimetric)

ELISA: Human VEGF 165 ELISA Kit (Colorimetric) [NBP2-79681] -

ELISA: Human VEGF 165 ELISA Kit (Colorimetric) [NBP2-79681] - Standard Curve Reference

Kit Contents for Human VEGF 165 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGF 165

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

Alternate Names

MVCD1, vascular endothelial growth factor A, Vascular permeability factor, VEGF-A, VEGFMGC70609, VPFvascular endothelial growth factor, biosimilar antibody, biosimilars

Gene Symbol

VEGFA

Additional VEGF 165 Products

Product Documents for Human VEGF 165 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human VEGF 165 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human VEGF 165 ELISA Kit (Colorimetric)

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  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

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