Vitamin D3 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB6566AFP680

R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 685503
View all Vitamin D3 Primary Antibodies »

Product Specifications

Specificity

Detects Vitamin D3 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Vitamin D3 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Vitamin D3

25-hydroxy-vitamin D3 (25(OH)D3) is synthesized in the liver and is the primary circulating form of vitamin D.  Its blood concentration, which reflects 25(OH)D3 produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status.  25(OH)D3 is metabolized to 1 alpha,25(OH)2D3 in the  proximal tubular cells of the kidney by the enzyme 1 alpha -hydroxylase.   The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure.  It has been shown that the active steroid hormone 1 alpha,25(OH)2D3 is essential for life in higher animals.  Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D3, 1 alpha,25(OH)2D3, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha -hydroxylase is present.

Alternate Names

Calcifediol, Calciol, Calcitriol, Cholecalciferol, VD3

Additional Vitamin D3 Products

Product Documents for Vitamin D3 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Vitamin D3 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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