Vitamin D3 Antibody

Catalog # Availability Size / Price Qty
Detection of Vitamin D3 in Human monocyte-derived dendritic cells by Flow Cytometry.
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Product Details
Supplemental Products

Vitamin D3 Antibody Summary

Detects Vitamin D3 in flow cytometry.
Monoclonal Mouse IgG1 Clone # 685503
Protein A or G purified from hybridoma culture supernatant
25-Hydroxyvitamin D3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Vitamin D3 antibody in Human monocyte-derived dendritic cells antibody by Flow Cytometry. View Larger

Detection of Vitamin D3 in Human monocyte-derived dendritic cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Vitamin D3 Monoclonal Antibody (Catalog # MAB6566, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D3

25-hydroxy-vitamin D3 (25(OH)D3) is synthesized in the liver and is the primary circulating form of vitamin D.  Its blood concentration, which reflects 25(OH)D3 produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status.  25(OH)D3 is metabolized to 1 alpha,25(OH)2D3 in the  proximal tubular cells of the kidney by the enzyme 1 alpha -hydroxylase.   The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure.  It has been shown that the active steroid hormone 1 alpha,25(OH)2D3 is essential for life in higher animals.  Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D3, 1 alpha,25(OH)2D3, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha -hydroxylase is present.

Alternate Names
Calcifediol; Calciol; Calcitriol; Cholecalciferol; VD3; Vitamin D3

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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