WNK3 Antibody

Novus Biologicals | Catalog # NBP1-03340

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all WNK3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human WNK lysine deficient protein kinase 3 using the numbering given in entry NP_001002838.1 (GeneID 65267).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

198 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WNK3 Antibody

Western Blot: WNK3 Antibody [NBP1-03340]

Western Blot: WNK3 Antibody [NBP1-03340]

Western Blot: WNK3 Antibody [NBP1-03340] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WNK3 antibody used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Applications for WNK3 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WNK3

Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases thatlack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site.Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function (Holden et al.,2004 (PubMed 15194194)).(supplied by OMIM)

Alternate Names

EC 2.7.11, FLJ30437, KIAA1566FLJ42662, PRKWNK3lysine deficient 3, WNK lysine deficient protein kinase 3

Entrez Gene IDs

65267 (Human)

Gene Symbol

WNK3

UniProt

Q9BYP7 (Human)

Additional WNK3 Products

Product Documents for WNK3 Antibody

Certificate of Analysis

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Product Specific Notices for WNK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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