Multiplex Measurement of Human Kidney Biomarkers in Serum Using the Magnetic Luminex® Performance Assay

ABSTRACT

The kidneys play an important role in organismal homeostasis by regulating osmolality and blood pressure, aiding in the reabsorption of water and nutrients, excreting wastes, and secreting hormones. Renal function is also important in the metabolism and excretion of drugs; therefore, analyzing nephrotoxicity using renal biomarkers is an important experimental step during drug development. Historically, renal function has been evaluated by measuring serum creatinine and blood urea nitrogen levels. Recently, more sensitive kidney biomarkers have been identified including TIM-1/KIM-1/HAVCR, Lipocalin-2/NGAL, Osteopontin, Cystatin C, Clusterin, CXCL10/IP-10, RBP-4, Fetuin A/AHSG, and TFF3. Serum samples from 10 healthy individuals, 10 acute kidney injury (AKI) individuals, and 10 individuals diagnosed with renal failure were evaluated using the Human Kidney Biomarker Magnetic Luminex Performance Assay. We observed statistically significant differences in the AKI group compared to the healthy group for Lipocalin-2, Osteopontin, Fetuin A, and Cystatin C. We observed statistically significant differences in the renal failure group compared to the healthy group for TFF3, Lipocalin-2, Clusterin, Fetuin A, and Cystatin C. We also observed statistically significant differences in the AKI group compared to the renal failure group for the following biomarkers: TFF3, Fetuin A, and Cystatin C.

The Human Kidney Biomarker Magnetic Luminex® Performance Assay (R&D Systems, Catalog # LHK000) is designed for simultaneous quantitative determination of biomarkers in serum, plasma, or urine.

 Luminex® is a trademark of Luminex Corporation.