1M7

Key Product Details

Product Description

1M7 is a reagent for RNA SHAPE-MaP experiments. Enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.

Product Specifications for 1M7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for 1M7

The following data is based on the product molecular weight 222.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.50 mL	22.51 mL	45.01 mL
5 mM	0.90 mL	4.50 mL	9.00 mL
10 mM	0.45 mL	2.25 mL	4.50 mL
50 mM	0.09 mL	0.45 mL	0.90 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Smola Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 2015 PMID: 26544910
• Turner Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 2013 PMID: 24141619
• Siegfried RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 2014 PMID: 25028896
• Mortimer A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 2007 PMID: 17367143

Product Documents for 1M7