Single Cell Sorter and Dispenser
Gentle, Fluorescent Single Cell Isolation made Simple
What is the Pala Single Cell Sorter and Dispenser?
Pala is a gentle, microfluidic-based single cell dispenser that combines two-laser fluorescence sorting in a fast and easy to use interface. Isolating single cells with fluorescence markers has never been easier with a start-up time of less than 5 minutes and delivery of a 96-well plate of single cells in as little as 2 minutes. The two-laser configuration enables a broad range of fluorescence channels providing flexibility in experimental designs. Pala is extremely gentle on sensitive cell types, such as stem cells, exposing the cells to less than 2 PSI / 13.79 kPa of pressure.
Fluorescence Sorting
Two laser configurations:
488 nm (Blue) + 561 nm (Green): 9 channels
405 nm (Violet) + 488 nm (Blue): 11 channels
Gentle on Cells
Pala operates at <2 PSI / 13.79 kPa of pressure to preserve cell health and viability.
Simple to Use
Pala has a simple software interface that prompts users though critical start up, shutdown, or sample switch procedures. No specialized training is required.
How Does Pala Work?
Leverage the powerful combination of microfluidics, flow cytometry, and liquid dispensing in Pala:
- Liquid Dispensing: Isolate single cells into 96- or 384-well plates according to your fluorescence selection criteria.
- Microfluidic Cartridge: Enable sterile sorting and easy sample switching with no chance for carryover.
- Flow Cytometry: Detect fluorescent labels or expressed proteins and gate cells of interest for sorting.
Low Sample Input
High Recovery Near zero dead volume enables handling small sample inputs down to 100 cells.
High Throughput
Process more sample types, streamlining single-cell dispensing workflow with no cleaning or re-calibration in between.
No Sample Carryover
Disposable cell cartridge prevents cross-contamination between samples.
Low Dispense Volume
Single cells are contained in a 1 μL droplet.
Pala Single Cell Sorter and Dispenser
Configured with two lasers for up to 11 detection channels.
Microfluidic Cell Cartridges
Disposable and sterile cell cartridge preventing cross contamination.
Dr. Oscar Perez-Leal from Temple University describes how his team leveraged the Pala Single Cell Dispenser to improve clonal outgrowth of CRISPR-edited cells.
Dr. Lina Chakrabarti from AstraZeneca discusses how the single cell dispenser technology on the Pala boosted cell line development workflows for biopharmaceutical manufacturing.
Dr. Paula Lam from BioCell Innovations expounds on the successful use of Pala to sort 293T suspension clones, leading to the production of high lentiviral titers.